A simple,sensitive and rapid determination of moexipril in human plasma by a novel LC-MS/MS method using solid phase extraction technique

Abstract

The aim of this work was to develop a simple,sensitive and selective liquid chromatography tandem mass spectrometry assay for quantification ofmoexipril in human plasma. Analytes and the internal standard(stable labelled isotopes) from human plasma by using solid-phase extraction technique with the help of Waters Oasis ® HLB 1 cc (30 mg) extraction cartridge. The reconstituted samples were chromatographed on Zorbax XDB C18,4.6*50mm column by using a mixture of acetonitrile -5 mM ammonium acetate buffer (80:20, v/v)as the mobile phase at a flow rate of 0.6 mL/min.The calibration curve obtained was linear (r  0.99) over the concentration range of 0.102-101.389 ng/mL for moexipril. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.2 min for each sample made it possible to analyze more than 350 human plasma samples per day.