The reference electrode in an electrolyte solution affects the surface potential of the nanoFET (field-effect transistor) channel which can result in a false signal to be measured in the FET channel during bio-and chemical sensing experiments. In this reported work, it was demonstrated that different sensing results that were obtained were affected by the choice of the reference electrode through a pH and ion concentration sensing experiment. Three types of reference electrodes were compared: the SCE (saturated calomel reference electrode), the Ag/AgCl tube and the integrated Ag/AgCl electrode. The pH sensitivity equivalent to the surface potential was evaluated: 32.3, 34.6 and 16.3 mV/ pH in the case of the SCE, the refillable tube type Ag/AgCl and the integrated Ag/AgCl, respectively.
Introduction:The bio field-effect transistor (BioFET) is one of the most attractive sensors because of the advantages of system integration and direct electrical readout for charged biomolecule detection. Limitations exist in the development of practical applications of nanoFETs as biosensors, despite the simple detection principle to directly measure the field effects of the charges of biomolecules, because of the measuring conditions in which the sensors operate which is an electrolyte solution environment where many different ions exist, such as physiological saline [1]. Furthermore, the Debye screening effect, that the distance affected by the electrostatic force by the charge of biomolecules rapidly attenuates depending on the ion concentration in the buffer, is another topic of consideration for the application in biosensors. Also, the pH of the solution is an important factor because of its effect on the charge of the biomolecule, the isoelectric point (pI) [2]. Moreover, the electric potential exerted by the solution affects the channel of the nanoFET when the voltage is applied to the solution [3].In this Letter, we confirm the effect of reference electrodes by measuring the pH and the ion concentration sensitivity by applying a bias voltage to the electrolyte solution with different types of electrodes: the SCE (saturated calomel reference electrode), the Ag/ AgCl electrode (tube-type) and the microAg/AgCl electrode (pastetype) were used to compare characteristics. The solution gate voltage was applied to the FET channel by the reference electrodes to bias the nanoFET channel and to evaluate the pH sensitivity through the surface potential of the FET channel as well.