A simple reverse-phase high pressure liquid chromatographic determination of conjugated bile acids in serum and bile using a novel radial compression separation system

Ruben, A. Th.; Berge‐Henegouwen, G. P. Van

doi:10.1016/0009-8981(82)90403-x

Cited by 89 publications

(15 citation statements)

References 14 publications

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“…Methanol/phosphate buffer was used as solvent (pH 5.2, flow rate of 1 mL/min) with detection at 200 nm. 35 Cumulative hydrophobicity index for bile salt species was calculated according to Heuman. 36 Protein Analysis.…”

Section: Patientsmentioning

confidence: 99%

Cholesterol crystallization in human gallbladder bile: Relation to gallstone number, bile composition, and apolipoprotein E4 isoform

Erpecum¹,

Berge‐Henegouwen²,

Eckhardt³

et al. 1998

Hepatology

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Patients with multiple cholesterol gallstones are at increased risk of recurrence after nonsurgical therapy, possibly because of fast biliary cholesterol crystallization. Serum apolipoprotein E4 (apo E4) is a risk factor for primary cholesterol gallstone formation as well as recurrence. We examined potential effects of stone number and apolipoprotein E genotype on crystallization and on various crystallization-influencing factors in gallbladder biles of 36 cholesterol stone patients (25 multiple stones: 10 carrying the ⑀4 allele). Biliary cholesterol saturation, bile salt composition or concentrations of total protein, immunoglobulin (Ig)A, IgG, ␣ 1 -acid glycoprotein, haptoglobin, or mucin-all crystallization promoters-did not differ between multiple and solitary stone patients, apparently not explaining different speed of crystallization (crystal observation time 3.5 ؎ 0.6 days vs. 12.7 ؎ 2.4 days, respectively; P ‫؍‬ .0003). In contrast, biliary aminopeptidase-N activities (2,607 ؎ 592 mU/mL vs. 947 ؎ 185 mU/mL; P ‫؍‬ .04) were higher and IgM levels (179 ؎ 39 vs. 65 ؎ 8 mg/L; P ‫؍‬ .09) tended to be higher in the case of multiple stones. Although patients carrying the ⑀4 allele had similar stone numbers and crystallization as patients without the ⑀4 allele, their cholesterol saturation index (CSI) was lower (1.08 ؎ 0.09 vs. 1.54 ؎ 0.13; P ‫؍‬ .01), whereas total protein and bile salt concentrations tended to be higher with preferential taurineconjugation. In conclusion, fast cholesterol crystallization is associated with multiple stones but not with apolipoprotein E4. Whereas fast crystallization may contribute to high recurrence rates after nonsurgical therapy in case of multiple gallstones, the mechanism for increased risk of gallstone formation in patients carrying the ⑀4 allele remains unknown. (HEPATOLOGY 1998;27:1508-1516.)Patients with multiple cholesterol gallstones have a higher risk of gallstone recurrence after successful nonsurgical treatment than patients with solitary stones. 1 Biliary cholesterol crystallization is much faster in the case of multiple than solitary cholesterol stones, 2,3 thus offering a potential explanation for high recurrence rates. In line with this finding, in a recent prospective study in gallstone patients treated by cholecystotomy with concomitant stone removal, fast crystallization in gallbladder bile obtained during the procedure was the only major risk factor for recurrence during follow-up. 4 Several factors may contribute to fast crystallization, such as biliary cholesterol supersaturation, increased concentration of bile in the gallbladder, 2,5 increased amounts of the hydrophobic bile salt deoxycholate, 6 a shortage of crystallizationinhibiting proteins, 7 or excessive crystallization promoting proteins. 8 The crystallization-promoting proteins have been partly isolated by means of lectin chromatography with concanavalin A-Sepharose. 8 This lectin binds glucose-and mannose-containing glycoproteins, which comprise about 10% of total biliary proteins. Increased tota...

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Section: Patientsmentioning

confidence: 99%

Cholesterol crystallization in human gallbladder bile: Relation to gallstone number, bile composition, and apolipoprotein E4 isoform

Erpecum¹,

Berge‐Henegouwen²,

Eckhardt³

et al. 1998

Hepatology

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“…Analysis of bile salt species was performed by HPLC using a modification of the method described by Ruben and BergeHenegouwen [16]. Bile samples were diluted 1 : 200 (v\v) in eluent (60 % methanol\40 % 3 mM K # HPO % , pH 3.75), and 20 µl portions of this sample were injected on a C18 Chromsphere column (5 µm pore size) (Chrompack International, Middelburg, The Netherlands) at a flow rate of 800 µl\min.…”

Section: Bile Analysismentioning

confidence: 99%

Regulation of MDR2 P-glycoprotein expression by bile salts

et al. 1995

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“…In healthy humans, biliary bile acids are sufficiently able to be determined in such procedures, but in other specimens, such as serum, HPLC requires a relatively large amount of specimens to accurately assay for the bile acid profiles (Ruben and Van Berge-Henegouwen 1982). An HPLC combined with an enzyme immobilized column using 3a-hydroxysteroid dehydrogenase has been reported to be a specific and much sensitive method for 3a-hydroxy bile acids (Okuyama 1976;Baba et al 1978; Mashige et al 1978 ; Onishi et al 1982).…”

Section: Discussionmentioning

confidence: 99%

Direct measurement of urinary bile acids of infants by high-performance liquid chromatography connected with an enzyme immobilized column.

Tazawa

Yamada

Nakagawa

et al. 1984

Tohoku J. Exp. Med.

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A simple reverse-phase high pressure liquid chromatographic determination of conjugated bile acids in serum and bile using a novel radial compression separation system

Cited by 89 publications

References 14 publications

Cholesterol crystallization in human gallbladder bile: Relation to gallstone number, bile composition, and apolipoprotein E4 isoform

Cholesterol crystallization in human gallbladder bile: Relation to gallstone number, bile composition, and apolipoprotein E4 isoform

Regulation of MDR2 P-glycoprotein expression by bile salts

Direct measurement of urinary bile acids of infants by high-performance liquid chromatography connected with an enzyme immobilized column.

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