A simple rat model ofin situreversible obstructive jaundicein situreversible obstructive jaundice model

Abstract

PurposeTo develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates.MethodsRats were divided into 4 groups with 8 rats each: the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed.ResultsOne rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group.ConclusionThe present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.

A Simple and Easily Reproducible Model of Reversible Obstructive Jaundice in Rats

“…With some modifications, including 4-0 polypropylene suture attached to the clip for pulling off use and the use of Seprafilm, we succeeded to develop a reliable reversible OJ model in the present study. Mortality as well as morbidity rate with 6.7% was considered acceptable compared to those of previously reported methods (16,17,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Furthermore, it was confirmed by either serum liver function tests or histopathology of the liver that OJ was properly induced and thereafter mitigated in our model.…”

“…In the current era, when NAC or conversion surgery after effective chemotherapy has been gaining wider acceptance, the basic research for investigating various effects of resolved OS on subsequent chemotherapy and/or surgery is considered very important. Hence, we attempted to acquire previously reported techniques for reversible OJ animal models (16,17,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Our group has reported the results of several studies using various animal hepatectomy and/or liver transplantation models (31)(32)(33)(34)(35)(36)(37)(38)(39)(40); thus, we recognized that our group has sufficient skill to reproduce these models.…”

“…Several reports on rat models of reversible OJ exist (16,17,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). The reports emphasized the simplicity of their methods.…”

“…The reports emphasized the simplicity of their methods. However, cutting and diverting or ligation and cannulation techniques reported by Costa et al and Huang et al were considered to require markedly refined surgical techniques (28,29). The "common bile duct ligation with splint" technique reported by Oruc et al was simple and excellent but used self-made elaborate material (20).…”

A novel murine model of reversible bile duct obstruction demonstrates rapid improvement of cholestatic liver injury

“…Additionally, current murine models of BDL do not replicate the ongoing reparative response that occurs alongside hepatic injury in human diseases in which bile flow is not completely obstructed as can occur in PSC, PBC, and biliary atresia (BA) after Kasai Portoenterostomy. While rat models of partial BDL (Jiang, Li, Wei, Jiang, & Miao, 2016) and reversal after BDL (BDLR) (Huang et al., 2017; Popov et al., 2010) have been established to evaluate the reparative processes in obstructive jaundice, limited models of BDL reversal have been established in mice. To overcome this limitation, murine models of BDL and BDLR have been established, however, prior models have used a cholecystojejunostomy to restore bile flow (Yang et al., 2014).…”