A simple, rapid polarographic method for blood oxygen content determination

Simple Methods for the Defermination of the Concentrations of Carbon Dioxide and Oxygen in Blood

“…amount of blood added to the saponin-ferricyanide solution it can be assumed that the solubility coefficient does not change significantly. (Neville (1960) showed this to be so for a similar mixture. )…”

“…The principle involved in the determination of the oxygen concentration (content) of blood has been described before (Baumberger, 1940) and was documented for use with a dropping mercury cathode oxygen electrode by Neville (1960). The method discussed here involved the use of a membrane-covered electrode thus eliminating the effect of the ferricyanide ions on the current flowing through the electrode.…”

Automated system for the absolute measurement of oxygen in whole blood

“…The advantages of this method over the Van Slyke method are mainly those provided by a design which lends itself to microprocessor control. These advantages include (1) operator independent accuracy and precision, (2) reduced measurement time, and (3) smaller sample size (which can be scaled down much further). However, the Van Slyke method has the inherent capability to measure contents of any blood gas for which chemical means exist to selectively remove it from all other gas components.…”

“…The volume at standard conditions corresponding to this pressure difference is calculated by use of the standard gas laws. The method is quite reproducible; however, it has been criticized for a 1 Present address: Department of Anesthesiology, 3C 205, University of Utah Medical Center, Salt Lake City, UT 84112. number of shortcomings (2)(3)(4) which include: (1) time requirement (15 min) for each determination, (2) tediousness of the procedure, and (3) extensive skill and experience requirement of technicians.…”

ELECTRODES FOR BLOOD AND GAS PCO2, Po; AND BLOOD pH

“…Beckman (a) and Chemtronics (d) both offer gas-phase oxygen electrodes with thermistor temperature compensation. The latter instrument, developed by Neville (22), utilizes a cadmium reference electrode to provide the operating bias voltage which eliminates the need for an external battery. The platinum is replaced by gold in his electrode.…”