A Simple Procedure for the Radioimmunoassay of 17_α-Hydroxyprogesterone in Serum: Comparison with an Immunological Purification Technique

Abstract

SUMMARY A simple radioimmunoassay for the measurement of l7cx-hydroxyprogesterone in 0'1-0·25 ml serum is described. An antibody prepared against 17cx-hydroxyprogesterone 3(0-carboxymethyl)-oxime-BSA as immunogen was used. A correlation of the 17ct:-hydroxyprogesterone concentration found in the serum of normal subjects and of patients with that obtained by a more complex immunological purification method was good (r=0'924, P < 0,001). Accuracy, precision, and specificity were acceptable with the simple method. Normal values for infants, children, and adults have been established, and the individual concentrations of 17cx-hydroxyprogesterone in the serum of patients with congenital adrenal hyperplasia were well separated from normal values.

Longitudinal Study of Plasma Cortisol and 17-Hydroxyprogesterone in Very-Low-Birth-Weight Infants during the First 16 Weeks of Life

“…Plasma 17-OHP was determined by a modification of a previously described radioim munoassay procedure [ 13]. The main changes were the use of ethyl acetate/n-hexane (1:1) as extraction sol vent and gelatin phosphosalinc for the radioimmu noassay buffer [14], The 17-OHP antiserum (Endo crine Sciences, Calabasas, Calif., USA) was raised against the conjugate 17-OHP-3-oxime-BSA and had cross-reactivities of 5% with 17-hydroxypregnenolone, 1% with progesterone and 1% with 11-deoxycortisol.…”

Prevalence of adrenal 21‐hydroxylase deficiency in neonates born in the West Midlands: a retrospective study

“…In 1980 a simple procedure for the measurement of serum 17a-hydroxy progesterone (17-OHP) was introduced that was suitable for monitoring 17-OHP concentrations in infants and children (Davila et al, 1980). Experience with this method to 1984 has demonstrated that it is useful for identifying infants at risk due to congenital adrenal hyperplasia (CAH), providing due care is taken in interpreting the results.…”