A simple, practical ‘swiss roll’ method of preparing tissues for paraffin or methacrylate embedding

Park, Carol M.; Reid, P. E.; Walker, David C.; MacPherson, Brian

doi:10.1111/j.1365-2818.1987.tb01321.x

Cited by 59 publications

(40 citation statements)

References 6 publications

(6 reference statements)

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“…The Swiss roll technique is the designated pathological procedure to enable localization of lesions precisely. 25 Thus far, no recurrent disease or distant metastases were observed in our patient group.…”

Section: Implications For Clinical Practice and Genetic Counselingmentioning

confidence: 98%

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CDH1‐related hereditary diffuse gastric cancer syndrome: Clinical variations and implications for counseling

Kluijt¹,

Siemerink

Ausems

et al. 2011

Intl Journal of Cancer

117

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CDH1 mutation carriers have a strongly increased risk of developing gastric cancer (GC) and lobular breast cancer (LBC). Clinical data of GC cases and surgical and histological data of prophylactic gastrectomies and mastectomies of all 10 Dutch CDH1 mutation families were collected. In vitro functional assays were performed to analyze the nature of the newly found missense mutation c.1748T>G (p.Leu583Arg). Ten different CDH1 mutations were found. Functional assays gave strong arguments for the pathogenic nature of the p.Leu583Arg mutation. The pedigrees comprised 36 GC cases (mean age 40 years, range 20–72 years) and one LBC case. Twenty‐nine/37 carriers alive, aged 18–61 years, underwent prophylactic gastrectomy. Invasive GC‐foci and premalignant abnormalities were detected in 2 and 25 patients, respectively. In four patients GC/signetring cell (SRC) foci were diagnosed at preoperative gastroscopy. Long‐standing presence of SRCs without progression to invasive carcinoma was shown in two others. Multifocal LBC/LCIS was found in the two prophylactic mastectomy specimens. Clefts of lip and/or palate (CL/P) were reported in seven individuals from three families. The age at onset and aggressiveness of GC is highly variable, which has to be included in counseling on planning prophylactic gastrectomies. The incidence of LBC is expected to increase and prophylactic mastectomy needs to be considered. The relationship between CL/P and CDH1 needs further study to inform future parents from hereditary diffuse gastric cancer (HDGC) families adequately.

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Section: Implications For Clinical Practice and Genetic Counselingmentioning

confidence: 98%

“…A Swiss roll technique 25 was used to study the complete mucosa of the gastrectomy specimens and all abnormalities were mapped ( Figs. 1 and 2).…”

Section: Follow-up In Cdh1 Positive Families Histological Evaluationmentioning

confidence: 99%

CDH1‐related hereditary diffuse gastric cancer syndrome: Clinical variations and implications for counseling

Kluijt¹,

Siemerink

Ausems

et al. 2011

Intl Journal of Cancer

117

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“…Upon sacrifice, the colon was isolated, and the length from cecum to anus measured. The colon was then opened longitudinally, the distal 1/3 isolated and snap-frozen for later analysis, and the remainder prepared in a "swiss roll" (Park, et al, 1987), placed into 10% neutral buffered formalin for 24h, then into 70% ethanol for an additional 24h.…”

Section: Murine Colitis Model and Assessmentmentioning

confidence: 99%

Alternative Medicine Products as a Novel Treatment Strategy for Inflammatory Bowel Disease

et al. 2008

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Inflammatory bowel disease (IBD) affects the mucosal lining of the gastrointestinal tract; the etiology is unknown and treatment is directed at systemic immunosuppression. Natural products, including medicinal herbs, have provided approximately half of the drugs developed for clinical use over the past 20 years. The purpose of our current study was to determine the effects of a novel combination of herbal extracts on intestinal inflammation using a murine model of IBD. Female Swiss-Webster mice were randomized to receive normal water or 5% dextran sulfate sodium (DSS) drinking water to induce colitis. Mice were treated with either a novel combination of herbal aqueous extracts or vehicle control per os (po) or per rectum (pr) every 24h for 7-8d. Disease activity index score (DAI) was determined daily; mice were sacrificed and colons analyzed by H&E staining, MPO assay, and cytokine (TNF-α, IL-6) ELISAs. Mice treated with the combination of herbal extracts, either po or pr, had significantly less rectal bleeding and lower DAI scores when compared to the vehicle-treated group. Moreover, colonic ulceration, leukocytic infiltration, and cytokine levels (TNF-α and IL-6) were decreased in the colons of herbal-treated mice, reflected by H&E staining, MPO assay, and cytokine ELISA. Treatment with the combination of medicinal herbs decreases leukocyte infiltration and mucosal ulceration, ameliorating the course of acute colonic inflammation. This herbal remedy may prove to be a novel and safe therapeutic alternative in the treatment of IBD.

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“…The 'swiss roll' technique may be used to visualize histological features along the length of the colon, but provides only a thin section through the tissue. 15 With transition from inflammation to neoplasia, dysplasia or cancer can affect any part of the colon with various magnitudes at different locations, complicating localization and characterization of disease by histopathology in pre-clinical studies. 5 Rapid imaging of bulk tissue along the length of freshly excised colon could provide sufficient information on crypt structure throughout the colon to characterize tissue in preclinical studies.…”

Section: Bowel Inflammationmentioning

confidence: 99%

Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope

et al. 2012

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Abstract. Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at 8; 333 lines∕sec by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of 7 mm∕sec. A single 1 × 60 mm 2 field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.

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A simple, practical ‘swiss roll’ method of preparing tissues for paraffin or methacrylate embedding

Abstract: SUMMARY A simple method of preparing ‘swiss rolls' from strips of tissue up to 500 mm in length is described. This procedure has been applied to a variety of tissues and is suitable for the preparation of both paraffin‐ and methacrylate‐embedded specimens.

Cited by 59 publications

References 6 publications

CDH1‐related hereditary diffuse gastric cancer syndrome: Clinical variations and implications for counseling

CDH1‐related hereditary diffuse gastric cancer syndrome: Clinical variations and implications for counseling

Alternative Medicine Products as a Novel Treatment Strategy for Inflammatory Bowel Disease

Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope

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