A simple-potentiometric method for determination of acid and alkaline phosphatase enzymes in biological fluids and dairy products using a nitrophenylphosphate plastic membrane sensor

Hassan, Saad S. M.; Sayour, Hossam E. M.; Kamel, Ayman H.

doi:10.1016/j.aca.2009.03.019

Cited by 53 publications

(25 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The volume of the required serum was 0.05 mL and the system can detect ALP levels in human serum samples at pathological and physiological levels, and also in detecting the iso-enzymatic forms of ALP. Hassan et al (2009) developed a new poly (vinyl chloride) matrix membrane detector responsive to 4-nitrophenylphosphate in assaying ALP and potentiometric assay [114]. The sensor was based on using an ion-association complex of 4-nitrophenylphosphate with a nickel (II)-bathophenanthroline cation as electrode active material, while the solvent mediator was nitrophenyloctyl ether.…”

Section: Electrochemistry Detection Techniquesmentioning

confidence: 99%

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Balbaied

Moore

2019

Biosensors

View full text Add to dashboard Cite

Alkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins, nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver, bone, kidney, and placenta) of almost all living organisms. This enzyme has been extensively used as a biomarker in enzyme immunoassays and molecular biology. ALP is also one of the most commonly assayed enzymes in routine clinical practice. Due to its close relation to a variety of pathological processes, ALP’s abnormal level is an important diagnostic biomarker of many human diseases, such as liver dysfunction, bone diseases, kidney acute injury, and cancer. Therefore, the development of convenient and reliable assay methods for monitoring ALP activity/level is extremely important and valuable, not only for clinical diagnoses but also in the area of biomedical research. This paper comprehensively reviews the strategies of optical and electrochemical detection of ALP and discusses the electrochemical techniques that have been addressed to make them suitable for ALP analysis in cell culture.

show abstract

Section: Electrochemistry Detection Techniquesmentioning

confidence: 99%

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Balbaied

Moore

2019

Biosensors

View full text Add to dashboard Cite

show abstract

“…At thetyrosinase composite electrode surface, quinine is reduced to cate cholth at is then reoxidized by tyrosinasetoquinone producing are doxcycle that produces the amplification of electro chemical signal obtained at the bio sensor and the sensitive detection of ALP Serra [85]; Fox and Kelly [32]. The use of 4-nitrophenyl phosphate as the substrate for a potential metric assessment of ALP in milk and dairy products has been described by Hassan [86] The sensor measures the decrease of a fixed concentration of 4-nitrophenylphosphate substrate as a function of the enzyme activity. This assay is reported to exhibit goods electivity and stability with a detection limit of 6.3×10−6M over the pH range of 4.5to10.The results obtained were reported to agree fairly well with data obtained from spectrophoto metric methods.…”

Section: Nonbovine Milk Productsmentioning

confidence: 99%

Validation of Milk Product Pasteurization by Alkaline Phosphatase Activity

Punoo

2018

CDVS

View full text Add to dashboard Cite

The standard practices for indirectly assessing the pasteurization status of milk products are primarily based on the thermal in activation kinetics of the endogenous milk enzyme, alkaline Phosphatase (ALP).This review provides an invaluable, tool for both regulatory and in house process control and validation. Endogenous milk ALP manifests a slightly higher heat resistance than the pathogenic micro flora upon which pasteurization time and temperature requirements are based. Therefore, ALP activity is recognized and accepted as the method of choice for the rapid validation of milk product pasteurization. However, ALP assays have notable limitations that must be understood if they are to be administered and interpreted correctly and the results are to be applied judiciously. Issues such as the reactivation of heat denatured ALP and the presence of both heat stable and labile microbial ALP are addressed. A discussion of ALP in the milk of non-bovine speciesis presented based on the limited literature available. Also brief discussion of research involving alternative pasteurization indicators is presented. This review article is intended to summarize the pertinent details of the ALP assay for dairy products (considering the basis and limitations of various methods) and the processing, handling, and known compositional factors that influence the assay results.

show abstract

“…Potential-based sensors or the so-called “ion selective electrodes” (ISEs) have been extensively introduced in different analytical applications, such as in clinical analysis [15,16,17,18,19], environmental monitoring [20,21,22,23,24,25], pharmaceutical analysis [26,27,28,29,30,31], and quality control criteria [32]. This class of analytical devices is characterized by their low cost, high reliability and validity, and ease of operation.…”

Section: Introductionmentioning

confidence: 99%

Single-Walled Carbon Nanotubes (SWCNTs) as Solid-Contact in All-Solid-State Perchlorate ISEs: Applications to Fireworks and Propellants Analysis

Hassan

Eldin

Amr

et al. 2019

Sensors

Self Cite

View full text Add to dashboard Cite

Herein, we present reliable, robust, stable, and cost-effective solid-contact ion-selective electrodes (ISEs) for perchlorate determination. Single-walled carbon nanotubes (SWCNTs) were used as solid-contact material and indium (III) 5, 10, 15, 20-(tetraphenyl) porphyrin chloride (InIII-porph) as an ion carrier. The sensor exhibited an improved sensitivity towards ClO4− ions with anionic slope of −56.0 ± 1.1 (R2 = 0.9998) mV/decade over a linear range 1.07 × 10−6 – 1.0 × 10−2 M and detection limit of 1.8 × 10−7 M. The short-term potential stability and the double-layer capacitance were measured by chronopotentiometric and electrochemical impedance spectroscopy (EIS) measurements, respectively. The sensor is used for ClO4− determination in fireworks and propellant powders. The results fairly agree with data obtained by ion chromatography.

show abstract

A simple-potentiometric method for determination of acid and alkaline phosphatase enzymes in biological fluids and dairy products using a nitrophenylphosphate plastic membrane sensor

Cited by 53 publications

References 40 publications

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Validation of Milk Product Pasteurization by Alkaline Phosphatase Activity

Single-Walled Carbon Nanotubes (SWCNTs) as Solid-Contact in All-Solid-State Perchlorate ISEs: Applications to Fireworks and Propellants Analysis

Contact Info

Product

Resources

About