A Simple Pore Water Hydrogen Diffusion Syringe Sampler

Vroblesky, Don A.; Chapelle, Francis H.; Bradley, Paul M.

doi:10.1111/j.1745-6584.2007.00362.x

Cited by 3 publications

(6 citation statements)

References 20 publications

(31 reference statements)

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“…Hydrogen is generally considered to be the ultimate electron donor for respiratory microbial chloroethene reductive dechlorination (Bradley 2003; Häggblom and Bossert 2003; Löffler et al 2003). The dissolved hydrogen concentration within each packed interval of 68BR was assessed using simple hydrogen diffusion syringe samplers as described elsewhere (Vroblesky et al 2007). Duplicate high‐density polyethylene syringe samplers were attached to the stem of the packer string within each packed interval and deployed at depth for 1 year.…”

Section: Methodsmentioning

confidence: 99%

“…Duplicate high‐density polyethylene syringe samplers were attached to the stem of the packer string within each packed interval and deployed at depth for 1 year. Upon string recovery, the syringes were detached from the packer assembly and their contents were analyzed by direct injection reduction gas detection gas chromatography (Chapelle et al 1997; Vroblesky et al 2007) within 30 min of recovery.…”

Section: Methodsmentioning

confidence: 99%

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Flowpath Independent Monitoring of Reductive Dechlorination Potential in a Fractured Rock Aquifer

Bradley¹,

Lacombe²,

Imbrigiotta³

et al. 2009

Groundwater Monitoring Rem

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The flowpath dependent approaches that are typically employed to assess biodegradation of chloroethene contaminants in unconsolidated aquifers are problematic in fractured rock settings, due to difficulties defining discrete groundwater flowpaths in such systems. In this study, the variation in the potential for chloroethene biodegradation with depth was evaluated in a fractured rock aquifer using two flowpath independent lines of field evidence: (1) the presence of the three biochemical prerequisites [electron donor(s), chloroethene electron acceptor(s), and chlororespiring microorganism(s)] for efficient chloroethene chlororespiration and (2) the in situ accumulation of chloroethene reductive dechlorination daughter products. The validity of this approach was assessed by comparing field results with the results of [1, 2‐14C] cis‐DCE microcosm experiments. Microcosms were prepared with depth‐specific core material, which was crushed and emplaced in discrete packer intervals for 1 year to allow colonization by the indigenous microbial community. Packer intervals characterized by significant electron donor concentrations, elevated numbers of chlororespiring microorganisms, and high reductive dechlorination product to parent contaminant ratios correlated well with the production of 14C‐labeled reductive dechlorination products in the microcosm experiments. These results indicate that, in the absence of information on discrete groundwater flowpaths, a modified approach emphasizing flowpath independent lines of evidence can provide insight into the temporal and spatial variability of contaminant biodegradation in fractured rock systems.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Flowpath Independent Monitoring of Reductive Dechlorination Potential in a Fractured Rock Aquifer

Bradley¹,

Lacombe²,

Imbrigiotta³

et al. 2009

Groundwater Monitoring Rem

Self Cite

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“…11 In order to test the passive diffusion sampling of hydrogen different kinds of hollow plastic bodies have been used. These bodies may be syringes 12,13 or tubings [14][15][16] from polypropylene or other semi-permeable materials. They are filled with an inert gas like nitrogen or helium and then deposited for a longer time in the aquifer.…”

Section: Environmental Impactmentioning

confidence: 99%

“…The gas chromatographic determination of hydrogen and methane was achieved using a pulsed discharge detector (PDD). 12,13 Methane was quantified in water samples by gas chromatographic headspace analysis using flame ionisation detection (FID). 17,18 A survey of the investigated sampling techniques and analytical methods is comprised in Table 1.…”

Section: Environmental Impactmentioning

confidence: 99%

The quantification of hydrogen and methane in contaminated groundwater: validation of robust procedures for sampling and quantification

Dorgerloh

Becker

Theissen³

et al. 2010

J. Environ. Monit.

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A number of currently recommended sampling techniques for the determination of hydrogen in contaminated groundwater were compared regarding the practical proficiency in field campaigns. Key characteristics of appropriate sampling procedures are reproducibility of results, robustness against varying field conditions such as hydrostatic pressure, aquifer flow, and biological activity. Laboratory set-ups were used to investigate the most promising techniques. Bubble stripping with gas sampling bulbs yielded reproducible recovery of hydrogen and methane which could be verified for groundwater sampled in two field campaigns. The methane content of the groundwater was confirmed by analysis of directly pumped samples thus supporting the trueness of the stripping results. Laboratory set-ups and field campaigns revealed that bubble stripping of hydrogen may be restricted to the type of used pump. Concentrations of dissolved hydrogen after bubble stripping with an electrically driven submersible pump were about one order of magnitude higher than those obtained from diffusion sampling. The gas chromatographic determination for hydrogen and methane requires manual injection of gas samples and detection by a pulsed discharge detector (PDD) and allows limits of quantification of 3 nM dissolved hydrogen and 1 µg L⁻¹ dissolved methane in groundwater. The combined standard uncertainty of the bubble stripping and GC/PDD quantification of hydrogen in field samples was 7% at 7.8 nM and 18% for 78 nM.

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“…Although dissolved fixed gases are known to be indicators, products, and substrates for important biogeochemical processes in both natural and contaminant-impacted groundwater – , their volatility and pressure-dependent solubility makes their sampling, handling, transport, and complete analyses challenging compared to that of nonvolatile dissolved constituents and contaminants. Recently, three techniques, using in situ syringe-based passive gas samplers – , have demonstrated their utility to collect, store, transport, and analyze dissolved gas samples. The recent approach of McLeish et al is particularly useful as the first published integrated sampling and analytical approach for many common dissolved gases in groundwater.…”

Section: Introductionmentioning

confidence: 99%

Passive Sampling and Analyses of Common Dissolved Fixed Gases in Groundwater

Spalding¹,

Watson²

2008

Environ. Sci. Technol.

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An in situ passive sampling and gas chromatographic protocol was developed for analysis of the major and several minor fixed gases (He, Ne, H2, N2, O2, CO, CH4, CO2, and N2O) in groundwater. Using argon carrier gas, a HayeSep DB porous polymer phase, and sequential thermal conductivity and reductive gas detectors, the protocol achieved sufficient separation and sensitivity to measure the mixing ratio of all these gases in a single 0.5 mL gas sample collected in situ, stored, transported, and injected using a gastight syringe. Within 4 days of immersion in groundwater, the simple passive in situ sampler, whether initially filled with He or air, attained an equivalent and constant mixing ratio for five of the seven detected gases. The abundant mixing ratio of N2O, averaging 2.6%, indicated that significant denitrification is likely ongoing within groundwater contaminated with uranium, acidity, nitrate, and organic carbon from a group of four closed radioactive wastewater seepage ponds at the Oak Ridge Field Research Center. Over 1000 passive gas samples from 12 monitoring wells averaged 56% CO2, 32.4% N2, 2.6% O2, 2.6% N2O, 0.21% CH4, 0.093% H2, and 0.025% CO with an average recovery of 95 +/- 14% of the injected gas volume.

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A Simple Pore Water Hydrogen Diffusion Syringe Sampler

Cited by 3 publications

References 20 publications

Flowpath Independent Monitoring of Reductive Dechlorination Potential in a Fractured Rock Aquifer

Flowpath Independent Monitoring of Reductive Dechlorination Potential in a Fractured Rock Aquifer

The quantification of hydrogen and methane in contaminated groundwater: validation of robust procedures for sampling and quantification

Passive Sampling and Analyses of Common Dissolved Fixed Gases in Groundwater

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