A simple polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the differentiation of cephalopod mollusc families Loliginidae from Ommastrephidae, to avoid substitutions in fishery field

Colombo, Fabio; Cerioli, M.; Colombo, Marco Maria; Marchisio, E.; Malandra, Renato; Renon, P

doi:10.1016/s0956-7135(01)00101-3

Cited by 19 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent advances in molecular techniques have greatly enhanced the identification of mollusk species, in particular the cephalopods (Brierly & Thorpe, 1994;Colombo et al, 2002;Santaclara, Espiñeira & Vieites, 2007;Leite, Haimovici, Molina & Warnke, 2008;Warnke, Soller, Blohm & Saint-Paul, 2000). The majority of studies have involved the identification of processed fishery products, based on comparative analyses of fragment length of mitochondrial DNA or nuclear genes, in particular 5S rDNA Céspedes et al, 1999;Cocolin, D'Agaro, Manzano, Lanari & Comi, 2000;Pinhal et al, 2008;Quinteiro et al, 1998;Russel et al, 2000;Wolf, Rentsch & Hübner, 1999).…”

Section: Introductionmentioning

confidence: 99%

Molecular differentiation of the species of two squid families (Loliginidae and Ommastrephidae) based on a PCR study of the 5S rDNA gene

et al. 2011

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Molecular differentiation of the species of two squid families (Loliginidae and Ommastrephidae) based on a PCR study of the 5S rDNA gene

et al. 2011

View full text Add to dashboard Cite

“…Different types of DNA makers have been widely used in the authentication of seafood products such as RFLP [13,103,104], RAPD [83,85], SSR [105] and SNP [84].…”

Section: Fishmentioning

confidence: 99%

DNA Markers for Food Products Authentication

Scarano

Rao

2014

Diversity

View full text Add to dashboard Cite

Media constantly refer of unscrupulous producers that adulterate, alter or replace premium products in food chains with the goal to maximize illegally profits. Food traceability is a central issue for the identification of improper labeling of processed food and feed and there are rules aimed to protect consumers and producers against fraudulent substitution of quality products in food chain, but the tools available are not always appropriate. DNA-based markers proved very effective for fresh and processed food molecular authentication. In this review, we illustrate potential and limits of different DNA markers focusing on low, medium and high-throughput markers, in order to monitor the genetic identity of food components in meat, fish and plants net-chains.

show abstract

“…In order to control these substitutions, a variety of genetic methods have been published for the identification of several cephalopod species. The majority of these are labor-intensive and time-consuming, such as forensically informative nucleotide sequencing (FINS), barcoding [10][11][12][13][14] and RFLP [8,15]. Some rapid DNA-based methods have also been published for the authentication of some cephalopod species [7,[16][17][18], but to date, there is not any rapid technique available for the genetic identification of S. officinalis.…”

Section: Introductionmentioning

confidence: 99%

A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products

Velasco

Ramilo-Fernández

Sotelo

2020

Foods

View full text Add to dashboard Cite

Cephalopods are very relevant food resources. The common cuttlefish (Sepia officinalis) is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authentication of S. officinalis in food products to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of S. officinalis showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify, or showed a significantly different Ct (p < 0.001). The calculated efficiency of the system was 101%, and the minimum DNA quantity detected was 10−4 ng. No cross-reactivity was detected with any other species, thus, the designed method differentiates S. officinalis from other species of the genus Sepia and other cephalopod species and works for fresh, frozen, grilled, cooked and canned samples of Sepia spp. The method has proved to be reliable and rapid, and it may prove to be a useful tool for the control of fraud in cuttlefish products.

show abstract

A simple polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the differentiation of cephalopod mollusc families Loliginidae from Ommastrephidae, to avoid substitutions in fishery field

Cited by 19 publications

References 6 publications

Molecular differentiation of the species of two squid families (Loliginidae and Ommastrephidae) based on a PCR study of the 5S rDNA gene

Molecular differentiation of the species of two squid families (Loliginidae and Ommastrephidae) based on a PCR study of the 5S rDNA gene

DNA Markers for Food Products Authentication

A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products

Contact Info

Product

Resources

About