A Simple Permanent Aceto-Orcein Stain for Free Cells, Cultures and Homogenates

Ritter, Hilda B.

doi:10.3109/10520295809111842

Cited by 8 publications

(2 citation statements)

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“…Observations of cells in mitosis were performed in smears prepared according to the method of Ritter (12). Freshly obtained ascitic fluid or concentrated balloon preparation was added to serological tubes containing lYo orcein in 45Yo acetic acid in the proportion of 1 volume of fluid to 5 volumes of the stain, and mixed well.…”

Section: Methodsmentioning

confidence: 99%

Cytological Studies of "Balloon Cells" Occurring in the Ehrlich Ascites Carcinoma

Tolnai¹

1964

Can. J. Biochem.

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Anomalous giant cells, termed "balloon cells", were found in a subliile of the Ehrlich ascites carcinoma. T h e balloon cells remained in the supernatant when ascitic fluid was centrifuged a t 17,000 r.p.nl.T h e balloon cells were considerably larger than the regular t~~i l l o r cells, a n d in their fully developed form the nucleus occupied a completely lateral position, resembling a signet-ring cell. T h e cytoplasnl contained only a small number of lipid granules surrounding the nucleus; no vacuolization of the cytoplasm could be demonstrated. Mucoid substances in the cytoplasnl were not accun~ulated. Mitochondria1 activity was localized mainly around the nucleus. 'The uptake of tritiated thymidine in the nucleus indicated colltillued D N A synthesis b y the balloons. They underwent mitotic division and contributed t o the expanding tumor cell population.

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Section: Methodsmentioning

confidence: 99%

Cytological Studies of "Balloon Cells" Occurring in the Ehrlich Ascites Carcinoma

Tolnai¹

1964

Can. J. Biochem.

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“…Differential counts of tumor cells, leukocytes, erythrocytes, and macrophages were made on wet prepara-Standard Deviation. tions of the ascitic fluid fixed and stained in 4 parts of a solution of 1 per cent aceto-orcein prepared as described by Ritter (15).…”

Section: Methodsmentioning

confidence: 99%

Some Characteristics of DNA Synthesis and the Mitotic Cycle in Ehrlich Ascites Tumor Cells

Edwards

Koch

Youcis

et al. 1960

The Journal of Cell Biology

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In ~vo studies of Ehrlich ascites tumor cells during the first ,5 days of growth in peritoneal cavities of mice consisted of the following:1. Determination of growth curves by direct enumeration of cells. 2. Estimation of the duration of each phase of the mitotic cycle based on incidence of cells in different phases.3. Radioautographic studies to determine the proportion of cells in different phases of the mitotic cycle that incorporate tritiated thymidine during a single brief exposure to this precursor of DNA.4. Estimation of the rate of incorporation of tritiated thymidine at different times during the period of DNA synthesis by comparison of mean grain counts over nuclei in radioautographs at different times following exposure to tritiated thymidine.The assumptions underlying these experiments and our observations concerning the duration of the period of DNA synthesis and its relation to the mitotic cycle are discussed. It is concluded that DNA synthesis is continuous, occupying a period of 8.5 hours during the interphase and that the average rate of synthesis is approximately constant.

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Chromosome morphology and species evolution in the five north American species of pacific salmon (Oncorhynchus)

Simon

1963

Journal of Morphology

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A Simple Permanent Aceto-Orcein Stain for Free Cells, Cultures and Homogenates

Cited by 8 publications

References 1 publication

Cytological Studies of "Balloon Cells" Occurring in the Ehrlich Ascites Carcinoma

Cytological Studies of "Balloon Cells" Occurring in the Ehrlich Ascites Carcinoma

Some Characteristics of DNA Synthesis and the Mitotic Cycle in Ehrlich Ascites Tumor Cells

Chromosome morphology and species evolution in the five north American species of pacific salmon (Oncorhynchus)

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