A simple PCR method for distinguishing Saccharomyces cerevisiae from its sibling species by amplification of the RPL2 region

Ryu, Seung-Lim; Mikata, Kozaburo; Murooka, Yoshikatsu; Kaneko, Yoshinobu

doi:10.1016/s0922-338x(98)80001-9

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…To date, several molecular approaches have been developed to distinguish among S. cerevisiae, S. bayanus, S. paradoxus and S. pasto- rianus : electrophoretic karyotyping, PCR fingerprinting, RFLP analysis of mitochondrial DNA and rRNA sequence analysis (de Barros Lopes et al, 1998 ;Guillamo! n et al, 1994 ;James et al, 1997 ;McCullough et al, 1998 ;Masneuf et al, 1996 ;Molnar et al, 1995 ;Montrocher et al, 1998 ;Naumov et al, 1992 b ;Nguyen & Gaillardin, 1997 ;Oda et al, 1997 ;Ryu et al, 1998 ;Valente et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Наумов¹,

James²,

Наумова³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

283

245

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Section: Introductionmentioning

confidence: 99%

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Наумов¹,

James²,

Наумова³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

283

245

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“…During the last 10 years, a number of PCR‐based procedures having different complexities and capacities for taxonomical resolution have been applied. These include species‐specific PCR (Ryu et al. 1998a; Josepa et al.…”

Section: Introductionmentioning

confidence: 99%

Rapid identification and differentiation of Saccharomyces cerevisiae, Saccharomyces bayanus and their hybrids by multiplex PCR

Torriani

Zapparoli

Malacrinò

et al. 2004

Lett Appl Microbiol

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Aims: To develop a multiplex PCR assay for the specific identification and differentiation of Saccharomyces cerevisiae, S. bayanus and their hybrids. Methods and Results: Two sets of primers with sequences complementary to the region YBR033w were used. A single amplicon of 1710 bp or 329 bp was obtained with species S. cerevisiae and S. bayanus, respectively, while the presence of both bands was observed in S. pastorianus because of its hybrid nature. Both amplification products were also obtained after amplification from DNA of several laboratory S. cerevisiae · S. bayanus hybrid strains. Conclusions: Multiplex PCR was optimized for the rapid and reliable identification of S. cerevisiae, S. bayanus and their hybrids. Significance and Impact of the Study: The procedure may be used for routine detection of the most common Saccharomyces sensu stricto yeasts involved in industrial fermentation processes, overcoming the problems of conventional techniques.

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“…25) On the other hand, Ryu et al reported that the 1,100-kb chromosome of S. bayanus strains, on which the HO gene located, was rearranged from chromosome II and chromosome IV of S. cerevisiae. 21,24) Our result, that the 1,100-kb chromosomes of S. uvarum carried the Uv-HO gene, suggests that the same chromosome rearrangement occurred in S. uvarum as in S. bayanus var. bayanus, and that the strains might share a similar chromosome constitution, but nucleotide sequence of the Uv-HO gene lost homogeneity from that of the Sb-HO gene by accumulation of sequence alterations.…”

Section: Discussionmentioning

confidence: 66%

“…This method has advantages over the PCR-RFLP 22) of the rDNA spacer region, and the previous PCR 21) detecting the chromosome rearrangement occurring in S. bayanus species. Our PCR analysis showed the HO gene polymorphism in S. bayanus species; the S. globosus type strain had only the Sb-HO gene, type strains of S. heterogenicus and S. uvarum had only the Uv-HO gene, and type strains of S. bayanus and S. inusitatus had both the Sb-HO gene and the Uv-HO gene.…”

Section: Discussionmentioning

confidence: 99%

“…The 0.52-kb DNA fragment of the Uv-HO gene was amplified from NBRC10549, NBRC10550, and NBRC10551 (lanes 13-15 respectively), which were detected to have the S. bayanus-specific chromosome rearrangement. 21) The 0.85-kb DNA fragments of Sb-HO were amplified from NBRC0676, NBRC1948, and NBRC10558 (lanes 9, 10, and 16 respectively). This result indicates that the Uv-HO gene exists widely in strains belonging to S. bayanus species.…”

Section: Uv-ho Gene Distribution Among Strains Classified As S Bayanmentioning

confidence: 99%

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A Structural and Phylogenetic Study of theHOGene fromSaccharomyces bayanusvar.uvarum

Tamai

Kanai

Kaneko

2007

Bioscience, Biotechnology, and Biochemistry

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A novel HO gene (Uv-HO) was cloned from the Saccharomyces bayanus var. uvarum (abbreviated as S. uvarum in this study) type strain. The coding region of Uv-HO showed relatively high homology (95%) to that of the Sb-HO gene (S. bayanus var. bayanus HO), but not to the HO genes of other Saccharomyces sensu stricto species. However, the 5' and 3' non-coding region of Uv-HO showed less similarity (79% and 76% respectively) even to those of the most homologous gene Sb-HO. Motifs of the mating-type control and the cell-cycle control were conserved in the 5' non-coding region of Uv-HO, but numbers and positions of motifs were different from those of Sb-HO. CHEF-Southern analysis showed that all tested strains of S. bayanus species, including S. uvarum, carried the HO gene on the 1,100-kb chromosome. By HO-typing PCR using mixed primers, which provided a rapid and convenient tool for yeast identification, either the Uv-HO gene or the Sb-HO gene was detected in strains of S. bayanus species, but two strains were found to have both types of HO gene in each genome. These results suggest that S. uvarum has a unique sequence, but might share the same chromosome constitution within S. bayanus species, and that S. bayanus is a heterogeneous species, of which some strains might be natural hybrid.

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A simple PCR method for distinguishing Saccharomyces cerevisiae from its sibling species by amplification of the RPL2 region

Cited by 8 publications

References 27 publications

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Rapid identification and differentiation of Saccharomyces cerevisiae, Saccharomyces bayanus and their hybrids by multiplex PCR

A Structural and Phylogenetic Study of theHOGene fromSaccharomyces bayanusvar.uvarum

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