A simple, novel and high efficiency sap inoculation method to screen for tobacco streak virus

Siddappa, Sundaresha; Sreevathsa, Rohini; Balol, Gurupada B.; Keshavareddy, G.; Rangaswamy, K. T.; Udayakumar, M.

doi:10.1007/s12298-012-0125-7

Cited by 11 publications

(7 citation statements)

References 10 publications

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“…In general, Ilarvirus has a wide host range as they are efficiently sap transmissible to many of the host plants belonging to Amaranthaceae, Chenopodiaceae, and Fabaceae. A rapid and efficient sap inoculation method for tobacco streak virus (TSV-SF) has been developed for screening a large number of sunflower geno types for resistance to the disease (Sundaresha et al 2012).…”

Section: Mechanical/sap Transmissionmentioning

confidence: 99%

Diseases of Edible Oilseed Crops

Chattopadhyay¹,

Kolte²,

Waliyar³

2015

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AgriculturalSciences. He has more than 22 years of research experience in oilseed crop disease management and has significantly contributed in the areas of understanding the host-pathogen interactions, epi demiology, and integrated disease management in sunflower, safflower, sesame, peanut, and rape seed-mustard crops. He has published more than 70 peer-reviewed research papers and 4 books. Dr. Chattopadhyay has to his credit several academic awards and one patent. His significant research achievements include the development of Fusarium wilt-resistant genotypes in safflower, the iden tification of genotypes of Brassica juncea resistant to a mixture of isolates of Albugo candida, the development of a biocontrol agent formulation to reduce the use of fungicides in the management of major diseases of oilseeds (Brassica), and seed treatment (and foliar application) of aqueous bulb extract of Allium sativum for the management of major foliar diseases (Alternaria blight, white rust, powdery mildew, and Sclerotinia stem rot) of mustard. S.J. Kolte, professor of plant pathology (Retd.

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Section: Mechanical/sap Transmissionmentioning

confidence: 99%

Diseases of Edible Oilseed Crops

Chattopadhyay¹,

Kolte²,

Waliyar³

2015

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“…Singh and Awasthi 33 reported different artificial mechanical transmission of virus like TMV, CMV, TSV, Okra mosaic virus, Papaya ringspot virus, Cowpea mosaic virus and alfalfa mosaic virus in tobacco, tomato, sunflower, okra, papaya, cowpea and potato (Sacrista´n et al 34 ; Jalender et al 35 ; Sundaresha et al 36 ; Givord and Hirth 37 ; Gonsalves 38 ; Surekha et al 39 ; El-Abhar et al 40 ). and wheat.…”

Section: Mechanical Transmission With Mymv Maintained Agrobacterium Clonesmentioning

confidence: 99%

Molecular Cloning of MYMV Genome and Infectivity of Yellow Mosaic Virus in Green Gram Using Different Viral Transmission Tools

Talakayala

Prathyusha

Divya

et al. 2021

Biosci., Biotech. Res. Asia

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Mungbean yellow mosaic virus (MYMV) causes massive crop losses in green gram. MYMV is a member of begomovirus with bipartite genome comprising DNA-A and DNA-B components, which is transmitted by whiteflies. Cloning and preparation of infectious clone is very much essential for screening germplasm or transgenic material of pulse crops since viruliferous whiteflies may not be available throughout the year. In the current work, we have amplified rolling circle mediated viral genome of MYMV using Φ29 DNA polymerase. The amplified products was digested and cloned into the plant expression vector pCAMBIA2301.The cloned constructs was then transformed into Agrobacterium LBA4404 through freeze thaw method. Further, three viral transmission techniques including mechanical rubbing, Agroinfiltration and Agroinoculation, were employed for assessing the mosaic symptoms in green gram. The molecular confirmation through polymerase chain reaction (PCR) indicated that the yellow mosaic symptoms were formed due to infectivity of MYMV in the green gram.

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“…Studies have shown that the selection of suitable plant tissues and inoculation sites is the key to successful infection. Commonly used tissues include the meristems of seedlings [ 23 ], three-week-old micro-shoots [ 24 ], and the stems or petioles of 4–6 leaf stage plants [ 25 ]. However, the use of such sites is complicated and time-consuming, increasing the operational complexity of inoculation [ 23 – 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…Commonly used tissues include the meristems of seedlings [ 23 ], three-week-old micro-shoots [ 24 ], and the stems or petioles of 4–6 leaf stage plants [ 25 ]. However, the use of such sites is complicated and time-consuming, increasing the operational complexity of inoculation [ 23 – 25 ]. Therefore, a rapid, simple, economical, and highly efficient VIGS and virus inoculation method is in need.…”

Section: Introductionmentioning

confidence: 99%

A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

Liu

et al. 2021

BMC Plant Biol

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Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants. Agrobacterium-based infiltration has been widely adopted as a tool for VIGS and in vitro-inoculation of plant virus. Most agrobacterium-based infiltration methods applied to VIGS and virus inoculation have the characteristics of low transformation efficiencies, long plant growth time, large amounts of plant tissue, large test spaces, and complex preparation procedures. Therefore, a rapid, simple, economical, and highly efficient VIGS and virus inoculation method is in need. Previous studies have shown that the selection of suitable plant tissues and inoculation sites is the key to successful infection. Results In this study, Tobacco rattle virus (TRV) mediated VIGS and Tomato yellow leaf curl virus (TYLCV) for virus inoculation were developed in tomato plants based on the agrobacterium tumefaciens-based infiltration by injection of the no-apical-bud stem section (INABS). The no-apical-bud stem section had a “Y- type” asymmetric structure and contained an axillary bud that was about 1–3 cm in length. This protocol provides high transformation (56.7%) and inoculation efficiency (68.3%), which generates VIGS transformants or diseased plants in a very short period (8 dpi). Moreover, it greatly reduces the required experimental space. This method will facilitate functional genomic studies and large-scale disease resistance screening. Conclusions Overall, a rapid, simple, and highly efficient method for VIGS and virus inoculation by INABS was developed in tomato. It was reasonable to believe that it can be used as a reference for the other virus inoculation methods and for the application of VIGS to other crops (such as sweet potato, potato, cassava and tobacco) that develop axillary buds and can survive from cuttings.

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A simple, novel and high efficiency sap inoculation method to screen for tobacco streak virus

Cited by 11 publications

References 10 publications

Diseases of Edible Oilseed Crops

Diseases of Edible Oilseed Crops

Molecular Cloning of MYMV Genome and Infectivity of Yellow Mosaic Virus in Green Gram Using Different Viral Transmission Tools

A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

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