A simple multinuclear NMR thermometer

Ammann, Claude; Meier, Pierre; Merbach, André E.

doi:10.1016/0022-2364(82)90147-0

Cited by 393 publications

(519 citation statements)

References 4 publications

Supporting

Mentioning

509

Contrasting

Order By: Relevance

“…Natural abundance and 2 H, 13 C, 15 N-labeled β1 immunoglobulin binding domain of protein G (GB1) were prepared according to previously published procedures [12,14]. Nano-crystalline GB1 samples were precipitated with natural abundance 2-methyl-pentane-2,4-diol (MPD) and isopropanol (IPA) [14].…”

Section: Methodsmentioning

confidence: 99%

“…However, the majority of experiments so far have been performed by detecting on either 13 C or 15 N nuclei, which are relatively insensitive due to their low gyromagnetic ratios. High-sensitivity detection on 1 H has only been realized recently in SSNMR.…”

Section: Introductionmentioning

confidence: 99%

“…The second approach is important for systems such as membrane proteins where deuteration and back-exchange may be challenging; it also permits all non-exchangeable protons to be investigated in a single experiment [13]. Both approaches result in a common outcome of greatly enhanced sensitivity relative to 13 C or 15 N detection.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High-performance solvent suppression for proton detected solid-state NMR

Zhou

Rienstra

2008

Journal of Magnetic Resonance

211

192

View full text Add to dashboard Cite

High-sensitivity proton-detected experiments in solid-state NMR have been recently demonstrated in proton-diluted proteins as well as fully protonated samples under fast magic-angle spinning. One key element for performing successful proton detection is effective solvent suppression achieved by pulsed field gradients (PFG) and/or saturation pulses. Here we report a high-performance solvent suppression method that attenuates multiple solvent signals simultaneously by more than a factor of 10,000, achieved by an optimized combination of homospoil gradients and supercycled saturation pulses. This method, which we call Multiple Intense Solvent Suppression Intended for Sensitive Spectroscopic Investigation of Protonated Proteins, Instantly (MISSISSIPPI), can be applied without a PFG probe. It opens up new opportunities for two-dimensional heteronuclear correlation spectroscopy of hydrated proteins at natural abundance as well as high-sensitivity and multidimensional experimental investigation of protein-solvent interactions.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High-performance solvent suppression for proton detected solid-state NMR

Zhou

Rienstra

2008

Journal of Magnetic Resonance

211

192

View full text Add to dashboard Cite

show abstract

“…[5]. Several similar relations for linking the frequency difference between both peaks of ethylene glycol to temperature can be found in literature (21)(22)(23). However, the resulting temperatures of these equations differ up to 1.3 degrees, which is much more than the intended accuracy of this measurement.…”

Section: Methodsmentioning

confidence: 80%

Compensating for magnetic field inhomogeneity in multigradient-echo-based MR thermometry

et al. 2014

View full text Add to dashboard Cite

Purpose: MR thermometry (MRT) is a noninvasive method for measuring temperature that can potentially be used for radio frequency (RF) safety monitoring. This application requires measuring absolute temperature. In this study, a multigradientecho (mGE) MRT sequence was used for that purpose. A drawback of this sequence, however, is that its accuracy is affected by background gradients. In this article, we present a method to minimize this effect and to improve absolute temperature measurements using MRI. Theory: By determining background gradients using a B 0 map or by combining data acquired with two opposing readout directions, the error can be removed in a homogenous phantom, thus improving temperature maps. Methods: All scans were performed on a 3T system using ethylene glycol-filled phantoms. Background gradients were varied, and one phantom was uniformly heated to validate both compensation approaches. Independent temperature recordings were made with optical probes. Results: Errors correlated closely to the background gradients in all experiments. Temperature distributions showed a much smaller standard deviation when the corrections were applied (0.21 C vs. 0.45 C) and correlated well with thermo-optical probes. Conclusion: The corrections offer the possibility to measure RF heating in phantoms more precisely. This allows mGE MRT to become a valuable tool in RF safety assessment. Magn

show abstract

“…In all measurements, the temperature was maintained by a Bruker B-VT 3000 temperature control unit, and was measured by a substitution technique. 43 Data treatment. Solomon-Bloembergen-Morgan (SBM) theory 6-7 has been used for data analysis (for equations see ref.…”

mentioning

confidence: 99%

Synthesis, complexation and NMR relaxation properties of Gd3+ complexes of Mes(DO3A)3

et al. 2011

View full text Add to dashboard Cite

Medium sized molecules endowed with multiple Gd 3+ complexes are efficient high magnetic field MRI contrast agents. The novel ligand Mes(DO3A) 3 , presenting three DO3A (1,4,7,10-tetraazacyclododecane-N,N¢,N¢¢-triacetatic acid) units grafted on the methyl positions of a central mesitylene (1,3,5-trimethylbenzene), has been synthesized. Designed as an MRI contrast agent, this ligand is complexed with Gd 3+ and its efficiency is characterized by variable field 1 H-NMR and variable temperature 17 O-NMR. The evaluation of the relaxation and paramagnetic chemical shift data allowed the identification of an undesired binuclear complex which is obtained by using the classical procedure for complexation as described in the literature. An intramolecular capping mechanism appears to be responsible for the failure to introduce a third Gd 3+ ion into the ligand. A new alternative method, based on pre-complexation with Mg 2+ followed by transmetallation is described here and leads to the expected trinuclear Gd 3+ complex [Mes{Gd(DO3A)(H 2 O) 2 } 3 ]. The rate constants for the water exchange (k ex 298 = 32 ¥ 10 6 s -1 ) for the bi-and trinuclear complex appeared to be the same, which is surprising in relation to the difference in the charge of the complex and to the difference in the number of coordinated water molecules, one and two per Gd 3+ for the binuclear and trinuclear complex, respectively.

show abstract

A simple multinuclear NMR thermometer

Cited by 393 publications

References 4 publications

High-performance solvent suppression for proton detected solid-state NMR

High-performance solvent suppression for proton detected solid-state NMR

Compensating for magnetic field inhomogeneity in multigradient-echo-based MR thermometry

Synthesis, complexation and NMR relaxation properties of Gd3+ complexes of Mes(DO3A)3

Contact Info

Product

Resources

About