A Simple Modification to the Luminometric Methylation Assay to Control for the Effects of DNA Fragmentation

Duman, Elif; Kriaucionis, Skirmantas; Dunn, John J.; Hatchwell, Eli

doi:10.2144/000114290

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…In addition, LUMA assays may differ from high-pressure liquid chromatography because the success of a DNA-digestion-based assay is dependent on the integrity of the DNA. 34 Additionally, epigenetic measures may be affected by differences in cell-type distribution of samples or tissue types assayed across studies which are not adjusted for in most studies. 21 One of the strengths of the current study was the evaluation of sensitive windows of micronutrient exposure and %5mC and % 5hmC at multiple time points to assess persistence of associations in childhood.…”

Section: Discussionmentioning

confidence: 99%

Prenatal trimester-specific intake of micronutrients: global DNA methylation and hydroxymethylation at birth and persistence in childhood

Parsons

Rifas‐Shiman²,

Bozack³

et al. 2022

J Dev Orig Health Dis

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The prenatal environment may program health and disease susceptibility via epigenetic mechanisms. We evaluated associations of maternal trimester-specific intake of micronutrients with global DNA methylation (%5mC) and 5-hydroxymethylation (%5hmC) at birth in cord blood and tested for persistence into childhood. We quantified global %5mC and %5hmC in cord blood cells (n = 434) and in leukocytes collected in early (n = 108) and mid-childhood (n = 390) from children in Project Viva, a pre-birth cohort from Boston, MA. Validated food frequency questionnaires estimated maternal first- and second-trimester intakes of vitamin B2, vitamin B6, vitamin B12, folate, betaine, choline, methionine, iron, and zinc. Mean (SD) cord blood %5mC and %5hmC was 5.62% (2.04) and 0.25% (0.15), respectively. Each μg increase in first-trimester B12 intake was associated with 0.002 lower %5hmC in cord blood (95% CI: −0.005, −0.0003), and this association persisted in early childhood (β = −0.007; 95% CI: −0.01, −0.001) but not mid-childhood. Second-trimester iron (mg) was associated with 0.01 lower %5mC (95% CI: −0.02, −0.002) and 0.001 lower %5hmC (95% CI: −0.01, −0.00001) in cord blood only. Increased second-trimester zinc (mg) intake was associated with 0.003 greater %5hmC in early childhood (β = 0.003; 95% CI: 0.0004, 0.006). Second-trimester folate was positively associated with %5hmC in early childhood only (β = 0.08, 95% CI: 0.003, 0.16). Associations did not survive multiple testing adjustment; future replication is needed. Trimester-specific nutrients may impact various sensitive windows of epigenetic programming some with lasting effects in childhood. Further research is needed to understand the role of gene-specific epigenetic changes and how global DNA methylation measures relate to child health.

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Section: Discussionmentioning

confidence: 99%

Prenatal trimester-specific intake of micronutrients: global DNA methylation and hydroxymethylation at birth and persistence in childhood

Parsons

Rifas‐Shiman²,

Bozack³

et al. 2022

J Dev Orig Health Dis

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“…While a daily intake of 200–320 μg folate was associated with a lower breast cancer risk, a daily intake of >400 μg significantly increased the risk 35 . Studies that focused on gene‐specific methylation, instead of global methylation, observed more consistent and reproducible findings 36 …”

Section: Role Of Biofactors In Cancer and Epigeneticsmentioning

confidence: 93%

The role of biofactors in the prevention and treatment of age‐related diseases

et al. 2021

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The present demographic changes toward an aging society caused a rise in the number of senior citizens and the incidence and burden of age‐related diseases (such as cardiovascular diseases [CVD], cancer, nonalcoholic fatty liver disease [NAFLD], diabetes mellitus, and dementia), of which nearly half is attributable to the population ≥60 years of age. Deficiencies in individual nutrients have been associated with increased risks for age‐related diseases and high intakes and/or blood concentrations with risk reduction. Nutrition in general and the dietary intake of essential and nonessential biofactors is a major determinant of human health, the risk to develop age‐related diseases, and ultimately of mortality in the older population. These biofactors can be a cost‐effective strategy to prevent or, in some cases, even treat age‐related diseases. Examples reviewed herein include omega‐3 fatty acids and dietary fiber for the prevention of CVD, α‐tocopherol (vitamin E) for the treatment of biopsy‐proven nonalcoholic steatohepatitis, vitamin D for the prevention of neurodegenerative diseases, thiamine and α‐lipoic acid for the treatment of diabetic neuropathy, and the role of folate in cancer epigenetics. This list of potentially helpful biofactors in the prevention and treatment of age‐related diseases, however, is not exhaustive and many more examples exist. Furthermore, since there is currently no generally accepted definition of the term biofactors, we here propose a definition that, when adopted by scientists, will enable a harmonization and consistent use of the term in the scientific literature.

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“…Distinct methylation profiles of various repetitive DNA elements have been found among different tissues and pathological conditions. Furthermore, loss of DNA integrity in cancer may compromise the accuracy of global DNA methylation assay outcome [142]. Thus, using different global DNA methylation assays to evaluate the effect of folate status on DNA methylation could be the reason behind the reported inconsistent and irreproducible findings.…”

Section: Micronutrients and Dna Methylation And Their Impact On Camentioning

confidence: 99%

Methyl Donor Micronutrients that Modify DNA Methylation and Cancer Outcome

2019

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DNA methylation is an epigenetic mechanism that is essential for regulating gene transcription. However, aberrant DNA methylation, which is a nearly universal finding in cancer, can result in disturbed gene expression. DNA methylation is modified by environmental factors such as diet that may modify cancer risk and tumor behavior. Abnormal DNA methylation has been observed in several cancers such as colon, stomach, cervical, prostate, and breast cancers. These alterations in DNA methylation may play a critical role in cancer development and progression. Dietary nutrient intake and bioactive food components are essential environmental factors that may influence DNA methylation either by directly inhibiting enzymes that catalyze DNA methylation or by changing the availability of substrates required for those enzymatic reactions such as the availability and utilization of methyl groups. In this review, we focused on nutrients that act as methyl donors or methylation co-factors and presented intriguing evidence for the role of these bioactive food components in altering DNA methylation patterns in cancer. Such a role is likely to have a mechanistic impact on the process of carcinogenesis and offer possible therapeutic potentials.

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A Simple Modification to the Luminometric Methylation Assay to Control for the Effects of DNA Fragmentation

Cited by 4 publications

References 10 publications

Prenatal trimester-specific intake of micronutrients: global DNA methylation and hydroxymethylation at birth and persistence in childhood

Prenatal trimester-specific intake of micronutrients: global DNA methylation and hydroxymethylation at birth and persistence in childhood

The role of biofactors in the prevention and treatment of age‐related diseases

Methyl Donor Micronutrients that Modify DNA Methylation and Cancer Outcome

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