A simple microfluidic dispenser for single-microparticle and cell samples

Kasukurti, A.; Eggleton, Charles D.; Desai, Sanjay A.; Disharoon, Dante; Marr, David W. M.

doi:10.1039/c4lc00863d

Cited by 14 publications

(9 citation statements)

References 35 publications

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“…Previously, Huang et al 26. and Kasukurti et al 27. respectively used optoelectronic or optical tweezers to push human ovarian cancer cells or red blood cells out of the microfluidic device and into a collection tube; Nakamura et al .…”

Section: Resultsmentioning

confidence: 99%

Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms

Zhang

Wang

Zhou

et al. 2017

Sci Rep

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Wider application of single-cell analysis has been limited by the lack of an easy-to-use and low-cost strategy for single-cell isolation that can be directly coupled to single-cell sequencing and single-cell cultivation, especially for small-size microbes. Herein, a facile droplet microfluidic platform was developed to dispense individual microbial cells into conventional standard containers for downstream analysis. Functional parts for cell encapsulation, droplet inspection and sorting, as well as a chip-to-tube capillary interface were integrated on one single chip with simple architecture, and control of the droplet sorting was achieved by a low-cost solenoid microvalve. Using microalgal and yeast cells as models, single-cell isolation success rate of over 90% and single-cell cultivation success rate of 80% were demonstrated. We further showed that the individual cells isolated can be used in high-quality DNA and RNA analyses at both gene-specific and whole-genome levels (i.e. real-time quantitative PCR and genome sequencing). The simplicity and reliability of the method should improve accessibility of single-cell analysis and facilitate its wider application in microbiology researches.

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Section: Resultsmentioning

confidence: 99%

Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms

Zhang

Wang

Zhou

et al. 2017

Sci Rep

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“… 56 Furthermore, the VCM can precisely steer the cells to different channels. This setup has been used to isolate microparticles and red blood cells nondestructively by controlling the OPU with an Arduino board (Arduino LLC, USA) in a gravity-driven microfluid device, 57 as shown in Figure 7 .…”

Section: Opu-based Biosensingmentioning

confidence: 99%

“…(B) Particles flowing into this section follow streamlines into the waste channel, unless translated by the optical trap into the sample channel that leads into a droplet section. Reprinted with permission from ref ( 57 ). Copyright 2014 Royal Society of Chemistry.…”

Section: Opu-based Biosensingmentioning

confidence: 99%

Hacking CD/DVD/Blu-ray for Biosensing

Hwu

Boisen

2018

ACS Sens.

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The optical pickup unit (OPU) within a CD/DVD/Blu-ray drive integrates 780, 650, and 405 nm wavelength lasers, diffraction-limited optics, a high-bandwidth optoelectronic transducer up to 400 MHz, and a nanoresolution x-, z-axis, and tilt actuator in a compact size. In addition, the OPU is a remarkable piece of engineering and could enable different scientific applications such as sub-angstrom displacement sensing, micro- and nanoimaging, and nanolithography. Although off-the-shelf OPUs can be easily obtained, manufacturers protect their datasheets under nondisclosure agreements to impede their availability to the public. Thus, OPUs are black boxes that few people can use for research, and only experienced researchers can access all their functions. This review details the OPU mechanism and components. In addition, we explain how to utilize three commercially available triple-wavelength OPUs from scratch and optimize sensing quality. Then, we discuss scientific research using OPUs, from standard optical drive-based turnkey-biomarker array reading and OPU direct bioapplications (cytometry, optical tweezing, bioimaging) to modified OPU-based biosensing (DNA chip fluorescence scanning, biomolecular diagnostics). We conclude by presenting future trends on optical storage devices and potential applications. Hacking low-cost and high-performance OPUs may spread micro- and nanoscale biosensing research from research laboratories to citizen scientists around the globe.

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“…While both chip-in-a-lab and lab-on-a-chip are categorised under micro total analysis systems, the former requires bulky equipment such as pumps while the latter uses the small size of the individual components in a chip. Many research reports on the applications of chip-in-a-lab were aimed at the separation of particle [3,4], bacteria [5,6] and cells [7,8].…”

Section: Introductionmentioning

confidence: 99%

Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre

et al. 2019

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This paper describes the development of an integrated system using a dry film resistant (DFR) microfluidic channel consisting of pulsed field dielectrophoretic field-flow-fractionation (DEP-FFF) separation and optical detection. The prototype chip employs the pulse DEP-FFF concept to separate the cells (Escherichia coli and Saccharomyces cerevisiae) from a continuous flow, and the rate of release of the cells was measured. The separation experiments were conducted by changing the pulsing time over a pulsing time range of 2–24 s and a flow rate range of 1.2–9.6 μ L min − 1 . The frequency and voltage were set to a constant value of 1 M Hz and 14 V pk-pk, respectively. After cell sorting, the particles pass the optical fibre, and the incident light is scattered (or absorbed), thus, reducing the intensity of the transmitted light. The change in light level is measured by a spectrophotometer and recorded as an absorbance spectrum. The results revealed that, generally, the flow rate and pulsing time influenced the separation of E. coli and S. cerevisiae. It was found that E. coli had the highest rate of release, followed by S. cerevisiae. In this investigation, the developed integrated chip-in-a lab has enabled two microorganisms of different cell dielectric properties and particle size to be separated and subsequently detected using unique optical properties. Optimum separation between these two microorganisms could be obtained using a longer pulsing time of 12 s and a faster flow rate of 9.6 μ L min − 1 at a constant frequency, voltage, and a low conductivity.

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A simple microfluidic dispenser for single-microparticle and cell samples

Cited by 14 publications

References 35 publications

Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms

Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms

Hacking CD/DVD/Blu-ray for Biosensing

Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre

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