A Simple Micro Cytotoxicity Test

Frequecies of the HLA‐C locus antigens, Cw 1, Cw2, Cw3, and Cw4, were determined in 88 patients with spondylitic diseases and 88 matched and 64 B27‐positive normal controls. Both Cw1 and Cw2 were significantly increased in B27‐positive patients and B27‐positive controls as compared to B27‐negative patients and matched controls. Cw1 and Cw2 demonstrate strong linkage disequilibrium with B27. Their absence in B27‐negative patients suggests disease susceptibility is related to HLA‐B.

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“…HLA typing was performed utilizing a standard microcytotoxicity method (16). Seven antisera were used to characterize the 4 C antigens studied.…”

Section: Methodsmentioning

confidence: 99%

Hla‐c locus antigens in hla‐b27 associated arthritis

Arnett

Hochberg

Bias

1978

Arthritis & Rheumatism

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“…In the absence of reliable biochemical data, it could provide the missing link between serology and genetics in the study of a complex antigenic system . Summary Molecular relationship of public H-2 antigens 1, 5,6,8,11,13,25, and 28 to private antigens controlled by K and D regions was studied using the technique of antibody-induced resistance to complement-mediated cytotoxicity . The results indicate physical association in the cell membrane between H-2 antigens 1…”

Section: Discussionmentioning

confidence: 99%

“…and 23 of H-2a, 8 and 31 of H-2d, 11 and 17 of H-2Q, 13 and 30 of H-2", 25 and 23 of H-2k, and 28 and 31 of H-2g. These results are in agreement with genetic mapping placing the determinants of antigens H-2 .8, 11, and 25 in the K region, the determinant of antigen H-2 .13 in the D region, and the determinants of antigens H-2 .1 and 28 in either the K or the D region.…”

mentioning

confidence: 95%

Molecular relationship between private and public H-2 antigens as determined by antigen redistribution method.

Hauptfeld¹,

Klein²

1975

The Journal of Experimental Medicine

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Classical H-2 antigens can be divided into two, not very sharply defined groups, private and public . Private H-2 antigens (1) have a restricted strain distribution : among inbred strains they are found in only one haplotype of independent origin ; public H-2 antigens (2), on the other hand, are shared by at least two, and quite often by a large number of unrelated haplotypes . Private H-2 antigens are believed to be controlled by alleles of two loci at the opposite ends of the H-2 complex, the H-2K locus in the K region, and the H-2D locus in the D region . In agreement with this belief, the antigens can be arranged in two series, where members of each series are mutually exclusive (3) . No such arrangement is possible for the public H-2 antigens and the nature of the genetic control of these antigens has long been a matter of controversy .According to the traditional interpretation of the H-2 complex (4), the public antigens are controlled by separate regions located between the K and D regions within the complex . According to the two-locus model (5), public H-2 antigens reside in the same molecules as the private ones and are controlled either by the H-2K or H-2D loci, or both . The two-locus model fits better the available data than the traditional multi-locus model (6) . Also, the former model is supported by the results of transplantation analysis demonstrating that only the peripheral regions of the H-2 complex are involved in rapid skin-graft rejection (7) . However, a formal proof that the serologically detectable public H-2 antigens are coded for by the K and D regions has never been provided . As a matter of fact, most recent developments (8, 9, cf. Discussion) suggest that an across the board assignment of public antigens to K and D regions could not be justified . Despite these developments, many investigators accept the two-locus model as an established fact and rely on it in their interpretation of the H-2 system . At this stage it is, therefore, critical to test whether the serologically detectable public H-2 antigens conform to the predictions of the two-locus model .We have recently developed a method that can be used to examine molecular relationships among various cell membrane antigens . The method is based on the observation that under certain circumstances, antigens coated with alloanti-* This investigation was supported by grant

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“…The original standard, the National Institutes of Health (NIH) -complement-dependent cytotoxicity (CDC) crossmatch, has been altered by longer incubation times, Amos-modified washes and the use of anti-human globulin (AHG) to enhance the sensitivity for detecting anti-HLA antibodies (1069,1070). Both immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies can cause a positive CDC crossmatch.…”

Section: The Evaluation Of Renal Transplant Candidates: Clinical Pracmentioning

confidence: 99%

The Evaluation of the Renal Transplant Candidates: Clinical Practice Guidelines

2001

American Journal of Transplantation

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A Simple Micro Cytotoxicity Test

Cited by 421 publications

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Hla‐c locus antigens in hla‐b27 associated arthritis

Hla‐c locus antigens in hla‐b27 associated arthritis

Molecular relationship between private and public H-2 antigens as determined by antigen redistribution method.

The Evaluation of the Renal Transplant Candidates: Clinical Practice Guidelines

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