A simple method to discriminate Guangchenpi and Chenpi by high-performance thin-layer chromatography and high-performance liquid chromatography based on analysis of dimethyl anthranilate

Li, Shang-Zhen; Guan, Xiaoxu; Zhao, Guang‐Chao; Lan, Hong-Ci; Yin, Qiang; Chu, Chu; Yang, Dongzi; Liu, E‐Hu; Zhou, Ping

doi:10.1016/j.jchromb.2019.121736

Cited by 15 publications

(10 citation statements)

References 13 publications

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“…D-Limonene (52.22-68.31%) was the most abundant volatile substance, followed by γ-terpinene (14.81-22.31%), ⊎-pinene (3.42-6.20%), ⊍-pinene (2.23-4.09%), methyl 2-(methylamino)benzoate (0.59-4.17%), terpinolene (1.00-1.87%), ⊎-myrcene (undetected-1.88%), ⊍-terpineol (0.43-1.51%) and 14 other volatile compounds with concentrations of less than 1%. It has been reported that methyl 2-(methylamino)benzoate is a characteristic component in CRCP, 36 which was detected in all samples in this study.…”

Section: Gc-ms Analysis Of Volatile Compounds During Harvestingsupporting

confidence: 58%

Evaluation of dynamic changes in the bioactive components in Citri Reticulatae Pericarpium (Citrus reticulata ‘Chachi’) under different harvesting and drying conditions

et al. 2020

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BACKGROUND: The Citrus reticulata 'Chachi' pericarp (CRCP) is one cultivar of Citri Reticulatae Pericarpium (Chenpi), which is widely applied in medicine and food. To determine the potential value of CRCP harvested at different stages and subjected to different drying processes, the dynamic changes in the bioactive components were profiled and evaluated in this study. RESULTS:The contents of all non-volatile components, i.e. synephrine, limonin, phenolic acids and flavonoids, decreased with delayed harvest time. The volatiles thujene, ⊍-pinene, ⊎-pinene, D-citronellol, D-citronellal, decanal, linalool, geraniol, L-cis-carveol, terpinen-4-ol, ⊍-terpineol, carvacrol, perillaldehyde, methyl 2-(methylamino)benzoate and D-limonene were considered the characteristic components for distinguishing CRCP harvested at different stages. Phenolic acids, synephrine and limonin were stable at different drying temperatures; however, high-temperature drying at 60°C induced a significant transformation in the flavonoids (especially polymethoxyflavones) and volatile substances in CRCP. CONCLUSIONS:The results suggested that most of the bioactive components declined with the growth of Citrus reticulata 'Chachi'. And it is believed that the fresh peel should be naturally sun-dried or dried at low temperature (30 or 45°C) rather than at high temperature (60°C) to prevent excessive loss of nutrients.

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Section: Gc-ms Analysis Of Volatile Compounds During Harvestingsupporting

confidence: 58%

Evaluation of dynamic changes in the bioactive components in Citri Reticulatae Pericarpium (Citrus reticulata ‘Chachi’) under different harvesting and drying conditions

et al. 2020

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“…Compounds Collected From UPLC-Q-TOF-MS/MS Analysis Identification technology of compounds and metabolites in CRP has developed rapidly, and mass spectrometry identification is a common technical method (Zheng et al, 2013;Zeng et al, 2017;Li et al, 2019a). We added an appropriate amount of methanol to prepare standard solutions of 50-100 μg/ml and accurately weighed CRP powder (1.0 g), placed it in a conical flask containing 80 ml methanol, mixed it well, treated it with an ultrasound machine for 30 min, passed the solution through filter paper, then increased the volume to 100 ml, and finally filtered the solution through a 0.22 μm microporous membrane to obtain the CRP extraction solution.…”

Section: Screening Of Active Compoundsmentioning

confidence: 99%

Systems Pharmacology Study of the Anti-Liver Injury Mechanism of Citri Reticulatae Pericarpium

Yang

et al. 2021

Front. Pharmacol.

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Liver diseases are mostly triggered by oxidative stress and inflammation, leading to extracellular matrix overproduction and prone to develop into liver fibrosis, cirrhosis and hepatocellular carcinoma. Liver injury (LI) refers to various pathogenic factors leading to the destruction of stem cells that then affect the liver’s normal function, causing a series of symptoms and abnormal liver function indicators. Citri Reticulatae Pericarpium (CRP) is one of the most commonly used traditional Chinese medicines; it contains flavonoids including hesperidin, nobiletin, and tangeretin. CRP has antibacterial, antioxidant, and antitumor effects that reduce cholesterol, prevent atherosclerosis and decrease LI. Here we analyzed the components of CRP and their targets of action in LI treatment and assessed the relationships between them using a systems pharmacology approach. Twenty-five active ingredients against LI were selected based on ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry results and databases. The drug targets and disease-related targets were predicted. The 117 common targets were used to construct a protein-protein interaction network. We identified 1719 gene ontology items in LI treatment, including 1,525 biological processes, 55 cellular components, and 139 molecular functions. These correlated with 49 Kyoto Encyclopedia of Genes and Genomes pathways. These findings suggest that CRP may counteract LI by affecting apoptotic, inflammatory, and energy metabolism modules. In vitro experiments suggested that the mechanism may involve hesperidin and naringenin acting on CASP3, BAX, and BCL2 to affect the apoptosis pathway, attenuating liver fibrosis. Naringenin significantly inhibited AKT1 phosphorylation, which in turn mediated activation of the phosphoinositide 3-kinase-Akt signaling pathways against LI. This study provides a reference for systematically exploring the mechanism of CRP’s anti-LI action and is also expands of the application of systems pharmacology in the study of traditional Chinese medicine.

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“…More recently, DH has garnered more attention due to its excellent and extensive bioactivity merits, such as improving human immunity, antidiabetes, hepatoprotective, anti-angiogenesis, anti-inflammatory, and anticancer benefits [12]. Emerging evidence has identified the bioactive ingredients of DH, such as polysaccharides, alkaloids, and flavonoids [13,14]. It is worthy that the polysaccharides as the main active compounds have exerted numerous functions including alleviating lung inflammation [15], inhibiting epithelial cell apoptosis [16], antioxidant [17], and regulating hepatic glucose homeostasis [18].…”

Section: Introductionmentioning

confidence: 99%

Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Fan

Han

Zhu

et al. 2020

Oxidative Medicine and Cellular Longevity

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Cardiovascular disease is the highest cause of death, and atherosclerosis (AS) is the primary pathogenesis of many cardiovascular diseases. In this study, we aim to investigate the possible pharmaceutical effects of Dendrobium huoshanense C. Z. Tang et S. J. Cheng polysaccharide (DHP) in AS. We fed zebrafish with high-cholesterol diet (HCD) to establish a zebrafish AS model and treated with DHP and observed plaque formation and neutrophil counts under a fluorescence microscope. Next, a parallel flow chamber was utilized to establish low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. We observed that DHP significantly improved HCD-induced lipid deposition, oxidative stress, and inflammatory response, mainly showing that DHP significantly increased superoxide dismutase (SOD) activity, decreased plaque formation, and decreased neutrophil recruitment and the levels of total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), and reactive oxygen species (ROS). Furthermore, DHP significantly improved LSS-induced oxidative stress and EC dysfunction. Our results indicated that DHP can exert treatment effects on AS, which may attribute to its hypolipidemic, antioxidant, anti-inflammatory activities and improving LSS-induced EC dysfunction. DHP has promising potential for further development as a functional natural medicine source targeted at AS prevention.

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A simple method to discriminate Guangchenpi and Chenpi by high-performance thin-layer chromatography and high-performance liquid chromatography based on analysis of dimethyl anthranilate

Cited by 15 publications

References 13 publications

Evaluation of dynamic changes in the bioactive components in Citri Reticulatae Pericarpium (Citrus reticulata ‘Chachi’) under different harvesting and drying conditions

Evaluation of dynamic changes in the bioactive components in Citri Reticulatae Pericarpium (Citrus reticulata ‘Chachi’) under different harvesting and drying conditions

Systems Pharmacology Study of the Anti-Liver Injury Mechanism of Citri Reticulatae Pericarpium

Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

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