A Simple Method of Intestinal Anastomosis (Ileocolostomy) in Rats

Inomata, Tomo; Ninomiya, Hiroyoshi; Mizutani, Tomoyuki; Mori, Yuko; Kashiwazaki, Naomi; Kiuchi, Akio; Nagai, Tetsushi

doi:10.1538/expanim.54.117

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…Furthermore, an increased presence of mitotic figures in aged tissue samples indicates a proliferation within the stem cell pool, suggesting that ageing‐induced cellular damage triggers stem cell activation. However, spectrochemical analyses reveal differential responses to plasma transfer in the ileum and colon tissues, given their distinct roles in digestion 36 . Unsaturated fatty acids have been shown to enhance endoplasmic reticulum function, while saturated fatty acids impair it by reducing membrane flexibility 37 .…”

Section: Discussionmentioning

confidence: 99%

“…However, spectrochemical analyses reveal differential responses to plasma transfer in the ileum and colon tissues, given their distinct roles in digestion. 36 Unsaturated fatty acids have been shown to enhance endoplasmic reticulum function, while saturated fatty acids impair it by reducing membrane flexibility. 37 Therefore, the decrease in saturated lipid content in the ileum tissue following young plasma transfer could be indicative of cellular regeneration.…”

Section: Discussionmentioning

confidence: 99%

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The rejuvenating influence of young plasma on aged intestine

Ceylani,

Teker,

Keskin

et al. 2023

J Cellular Molecular Medi

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This study aims to investigate the effects of plasma exchange on the biomolecular profiles and histology of ileum and colon tissues in young and aged Sprague–Dawley male rats. Fourier transform infrared (FTIR) spectroscopy, linear discriminant analysis and support vector machine (SVM) techniques were employed to analyse the lipid, protein, and nucleic acid indices in young and aged rats. Following the application of young plasma, aged rats demonstrated biomolecular profiles similar to those of their younger counterparts. Histopathological and immunohistochemical assessments showed that young plasma had a protective effect on the intestinal tissues of aged rats, increasing cell density and reducing inflammation. Additionally, the expression levels of key inflammatory mediators tumour necrosis factor‐alpha and cyclooxygenase‐2 significantly decreased after young plasma administration. These findings underscore the therapeutic potential of young plasma for mitigating age‐related changes and inflammation in the intestinal tract. They highlight the critical role of plasma composition in the ageing process and suggest the need for further research to explore how different regions of the intestines respond to plasma exchange. Such understanding could facilitate the development of innovative therapies targeting the gastrointestinal system, enhancing overall health during ageing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The rejuvenating influence of young plasma on aged intestine

Ceylani,

Teker,

Keskin

et al. 2023

J Cellular Molecular Medi

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“…Nevertheless, bowel preparation is also associated with complications, including dehydration and excessive flatulence. 3 In fact, the use of bowel preparation (primarily enema) to prepare patients for colorectal procedures has been the object of much discussion in the literature over the past few years. 3 The purpose of this experimental study was to compare the inflammatory and wound healing response of dogs submitted to colonic anastomosis with and without preoperative retrograde enema using a 10% glycerin solution.…”

Section: Introductionmentioning

confidence: 99%

Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs

Bezerra

Regadas

Figueiredo

et al. 2014

Journal of Coloproctology

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a b s t r a c tObjective: The purpose of this experimental study was to compare the inflammatory and wound healing response of dogs submitted to colonic anastomosis with and without preoperative retrograde enema. Methods:The study included two groups of 31 female dogs (Canis familiaris). G-I (control): no preoperative bowel preparation; G-II (study): preoperative retrograde enema using a 10% glycerin solution. All the animals were submitted to laparotomy and colotomy at 20 cm from the anal verge, followed by closure with a running extramucosal single-layer suture (Prolene ® 000). The animals were then anesthetized and euthanized on the 7th (n = 10) or 21st (n = 20) postoperative day (POD) to remove the anastomosed colon segment for histological and immunohistochemical analysis evaluating the parameters: anastomotic edema, vasoproliferation, abdominal adhesions, type I and III collagen, nitric oxide and myeloperoxidase.The observed differences were analyzed with the Mann-Whitney test for nonparametric data and Student's t test for unpaired samples and parametric data.Results: One animal from G-I and one from G-II died on POD 7 and POD 10 due to anastomotic complications and sepsis, respectively. The groups did not differ significantly with regard to inflammatory and healing parameters, although the levels of mature collagen were significantly lower in the animals submitted to preoperative bowel preparation. ଝ Study carried out at Post-graduation program stricto sensu in

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A Simple Method of Intestinal Anastomosis (Ileocolostomy) in Rats

Cited by 2 publications

References 21 publications

The rejuvenating influence of young plasma on aged intestine

The rejuvenating influence of young plasma on aged intestine

Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs

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