A Simple Method for the Production of Anti-C3
Antiserum in Rabbits

Merry, A.H.; Rawlinson, Violet I.; Stratton, F.

doi:10.1159/000466993

Cited by 1 publication

(2 citation statements)

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“…Such RBCs are resistant to conversion to C3d-RBCs by C3b inactivator, but are readily converted by trypsinization. (2) BMS-11 was produced using the same strategy as Merry et al, (8) but conducted to produce an MAb. The produced antibody belongs to the IgM class as determined by the Outherlony method.…”

Section: Resultsmentioning

confidence: 99%

“…It is well established that some components are capable of activating the human complement by an alternate pathway (AP) between the erythrocytes of different species. (8) The method described in this paper does not require the purification of C3 but relies on the attachment of C3b to mouse erythrocytes by activation of the AP and further conversion into C3d by the action of tripzin. In this paper full practical details of the method are given.…”

Section: Introduction S Ince the Small Subfragment Of C3 Remaining Onmentioning

confidence: 99%

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A Simple Method for the Production of Anti-C3d Monoclonal Antibody

Cruz

León

2007

Hybridoma

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Production of monoclonal antibodies to C3d usually involves the purification of protein. Our method does not require C3 purification; it relies on attachment of C3b to mouse erythrocytes by activation of alternative pathways and further conversion in C3d. We prepared human complement-coated mouse red cells and sensitized mice of the same strain with our own schedule of immunization and applied the classical methods to obtain a mouse monoclonal antibody. We obtained a clone called BMS-11 which produces a monoclonal antibody of IgM class, to C3d with a title of 1:500000. The monoclonal antibody obtained has shown that it is suitable for use as an antiglobulin reagent.

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Section: Resultsmentioning

confidence: 99%

Section: Introduction S Ince the Small Subfragment Of C3 Remaining Onmentioning

confidence: 99%