A Simple Method for the Rapid and Selective Absorption of Warm-Reactive Erythrocyte Autoantibodies from Serum

Abstract

A new technique has been developed to absorb selectively autoantibodies from sera. Red cells maximally coated with IgG antibodies are subjected to discontinuous, combined density and pH gradient centrifugation. The method yields a large quantity of antibody- poor, intact red cells that retain antigenicity. Red cells prepared in this manner absorb antibody as efficiently as do uncoated red cells and nonspecific absorption does not occur. Preliminary studies show that the method can be used to identify alloantibodies present in the sera of patients with ‘warm’ antibody-induced hemolytic anemia because the sera can be absorbed free of autoantibodies.

Antigen‐independent reactivation of anti‐E, years after allogeneic bone marrow transplantation: a case report

“…Some serum samples frozen at the t i e of BMT were tested in the gel sedimentation technique (DiamedR, Germany). Elution of the RBC autoantibodies and autoadsorption of autoantibodies to detect alloantibodies was performed using a discontinuous, combined density and pH gradient centrifugation (Hanfland, 1982). Platelet autoantibodies were determined by immunofluorescence (Schneider & Schnaidt, 1981) and the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) test (Kiefel et al, 1987).…”