A Simple Method for the Separation of Pregnanetriols by Spread-Layer Chromatography

Abstract

SUMMARY A method for the determination of urinary pregnanetriol is described which, by utilizing many features of the Fotherby & Love modification (1960) of Stern's method (1957) and by separation of pregnanetriol isomers by spread-layer chromatography of alumina, enables individual pregnanetriols and pregnanediol to be measured concomitantly.

Steroids: Group Estimation and Systematic Separation

“…In its adhering form, it is proving useful in group estimation procedures as illustrated by Starka and Malikova's (1961) pregnanetriol procedure (page 132). It is likely to be very useful in the II-oxygenation index determination where II-oxy-17-ketosteroids and Il-deoxy-17-ketosteroids may each be determined as a group.…”

Reproduction

“…Application of 17-ketosteroid analysis to peripheral and ovarian venous blood of twenty women demonstrated a consistently higher concentration in the ovarian vein samples (266). A new method, in volving spread layer chromatography on alumina, allows the separation of individual urinary pregnanetriols and of pregnanediol (268) . A new method, in volving spread layer chromatography on alumina, allows the separation of individual urinary pregnanetriols and of pregnanediol (268) .…”

Chromatography

“…The adsorption isotherms of corticosteroids have been determined by Urzua (1211). Adsorption chromatography on alumina is still widely used for the separation of estrogens (549,831,990), androgens (309,798), and progesterone metabolites (331,676,725), but silica also enjoys well deserved popularity as adsorbent (535,826,856,1308,1309) or carrier of the stationary phase of partition systems For weakly polar steroids, thin-layer chromatography (56,58,355,568,1120,1213,1214) has been found to be most useful; also spread-layer chromatography (195,473,1125), an analogous technique in which the adsorbent is not mixed with a binding agent. Other variants of this principle that have been successfully applied are glasspaper chromatography (263,443,1129,1161) and adsorption chromatography on impregnated (505,875) or nonimpregnated (198,801,865,968) paper.…”