A Simple Method for Short-Term Preservation of a Liver Homograft1

Schalm, S. W.; Terpstra, J. L.; Drayer, B.; Berg, C. van den; Veltkamp, J. J.

doi:10.1097/00007890-196912000-00014

Cited by 24 publications

(6 citation statements)

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“…[8] obtained better results. Trans planted livers functioned 4 h after gravitational perfusion and storage at 4 C. The same results were obtained after a warm ischaemia period of 10 min and 3 h of hypothermia [12]. A liver preserved in this way by Orr el al.…”

Section: Discussionsupporting

confidence: 54%

Electron Microscopic, Microangiographic and Cytophotometric Studies on Dog Livers after Various Methods of Preservation

Paquet¹,

Wessel²

1972

Eur Surg Res

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A report is made on the results of microangiographic, electron microscopic and cytophotometrie studies on preserved dog livers. The preservation methods employed were storage at 4 °C, isolated normothermic perfusion with diluted human blood in an oxygenator-pump system and the combination of hypothermia and hyperbaric oxygenation. Each preservation method was preceded by a 30-min gravitational perfusion (10 °C). In so far as the methods permitted homotransplantation, this was performed orthotopically. Four animals died due to preservation injuries of the transplant caused by an outflow block; the 5th dog lived for 2 days with a functioning transplant. Hypothermia allowed an ischaemic time up to 2 h whereas the combination of hypothermia and hyperbarism (4.5 atm), as well as the isolated heterologous normothermic perfusion prolonged the tolerance of ischaemia up to 4 h. Microangiographic and electron microscopic examinations showed a good correlation with the results of the transplantation as well as with the liver function tests during isolated perfusion with diluted blood. The filling of the sinusoids is evidence of good preservation; the commencement of the transformation of the glycogen fields into vacuoles and the swelling of the mitochondria are the first signs of injuries due to preservation. A decrease in the vitality of the liver cells is accompanied by a loss of Feulgen-positive substance in the liver cytoblast.

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Section: Discussionsupporting

confidence: 54%

Electron Microscopic, Microangiographic and Cytophotometric Studies on Dog Livers after Various Methods of Preservation

Paquet¹,

Wessel²

1972

Eur Surg Res

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“…We have so far carried out only 2 12-hour storages, one of which was completely success ful and the other partially so. The results of group III are a little better than those of Schalm [6] using simple cooling followed by instillation of plasma -bicarbonate -dextrose -procaine mixture. We would agree that the method we are investigating is complex but it does aim for a longer storage period, which we think is necessary for future clinical practice.…”

Section: Discussionmentioning

confidence: 80%

A Transportable Apparatus for Liver Preservation

et al. 1970

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“…After removing the liver from the animal, flushing was continued over 10 min (total volume of 1 liter). After that the liver was filled up with one of the following preservation solutions (400 ml) and stored at 4 °C for 6 h: (1) Plasma protein fraction 5% (PPF) [12], n = 5; additives per liter: 4.2 mmol MgS04, 7.5 mmol K2H P 0 4, 21.6 mmol HC1, 2,000 IU heparin, 250 mg hydrocortisone, 1 g ampicillin, 4.4 mmol glucose. (2) Albumin 5% (A), n = 7; addi tives as in 1.…”

Section: Methodsmentioning

confidence: 99%

Adenine Nucleotides and Glycolysis during Liver Preservation by Simple Hypothermic Storage

Otto¹,

Wolff²,

Hacker³

1984

Eur Surg Res

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Adenine nucleotides and glycolytic metabolites were examined during hypothermic storage of porcine livers by different preservation methods. The parameters ATP, ADP, AMP, G6P, F6P, FDP, GAP, 2PG, PEP, Pyr and Lact were investigated. The results indicate a dependence of adenine nucleotides but no dependence of glycolytic metabolites on preservation method. Storage by plasma protein fraction or Collins C2 solution causes a better result in TAN concentration after 6 h than albumin and Ringer storage or Ringer trickle perfusion. Conditioning the organ for a better outcome of preservation aimed at increasing the glycolytic rate is of questionable value.

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A Simple Method for Short-Term Preservation of a Liver Homograft1

Cited by 24 publications

References 0 publications

Electron Microscopic, Microangiographic and Cytophotometric Studies on Dog Livers after Various Methods of Preservation

Electron Microscopic, Microangiographic and Cytophotometric Studies on Dog Livers after Various Methods of Preservation

A Transportable Apparatus for Liver Preservation

Adenine Nucleotides and Glycolysis during Liver Preservation by Simple Hypothermic Storage

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