A Simple Method for Screening of Fresh Plant Material for Glycosidic Bound Volatile Compounds<sup>1</sup>

Ab, Svendsen; Ij, Merkx

doi:10.1055/s-2006-961772

Cited by 32 publications

(8 citation statements)

References 4 publications

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“…49290, Fluka) at 28°C for 16 h and the volatile compounds were collected in n-hexane (0.5 ml) by hydrodistillation. 28 All the essential oils and the volatile fractions were analysed by GC and GC-MS.…”

Section: Isolation Proceduresmentioning

confidence: 99%

Volatile constituents of different organs of Psoralea bituminosa L.

Bertoli

Menichini

Noccioli

et al. 2004

Flavour & Fragrance J

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The essential oil and SPME samples of the leaves, flowers and seeds of Psoralea bituminosa L. were analysed by GC and GC-MS. We have investigated also the presence of monoterpene or aliphatic alcohol glucosides. The essential oils showed both qualitative and quantitative differences. The main constituents of the leaf and the flower essential oils were caryophyllene (23% and 18%, respectively), β-farnesene (15% and 6%, respectively), and germacrene D (24% and 18%, respectively). Significant amounts (7%) of the same compounds were also directed in the seed essential oil, but tricyclene (11%) and α-pinene (50%) were the most important constituents of this oil. The volatile fractions of remaining leaf aqueous extracts after treatment with β-glucosidase revealed qualitative differences in comparison with the composition of the corresponding essential oils, and high levels of 3-hexen-1-ol (37%) and 1-octen-3-ol (27%) were observed. The SPME analysis of the fresh leaves, flowers and seeds of P. bituminosa confirmed the qualitative composition of the volatile oils, even if we detected significative differences in the percentage ratio between monoterpenes and sesquiterpenes in comparison with the oils, where sesquiterpenes were the main components. In fact the variation of the monoterpenes, tricyclene, α-pinene and camphene between the leaf oil and the corresponding headspace sample was remarkable: tricyclene increased from 0.1% to 8%, α-pinene from 0.1% to 16% and camphene from 0.3% to 10% in the SPME samples.

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Section: Isolation Proceduresmentioning

confidence: 99%

Volatile constituents of different organs of Psoralea bituminosa L.

Bertoli

Menichini

Noccioli

et al. 2004

Flavour & Fragrance J

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“…The remaining aqueous extracts were treated with β-glycosidase at 28 °C for 16 h and the volatile compounds produced by enzymatic hydrolysis were collected in n-hexane (0.5 ml) by hydrodistillation. 24 All the essential oils and the volatile fractions obtained by hydrolysis were analysed by GC and GC-MS.…”

Section: Isolation Proceduresmentioning

confidence: 99%

Volatile constituents of the leaves and flowers of Hypericum triquetrifolium Turra

Bertoli

Menichini

Mazzetti

et al. 2003

Flavour & Fragrance J

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The flower and leaf oil of Hypericum triquetrifolium Turra (Guttiferae, Hypericoideae) from Calabria (Italy) were studied by GC and GC-MS. The major components identified in each oil were n-nonane (8%, 15%), β-pinene (8%, 4%), α-pinene (13%, 10%), myrcene (16%, 5%), β-caryophyllene (5%, 11%), germacrene-D (10%, 13%), sabinene (13%, 3%) and caryophyllene oxide (5%, 12%) in the leaf and flower oils, respectively. The aerial parts of the same plant were also analysed by SPME. The SPME analysis showed higher yields of undecane (14%, 10%) and β-caryophyllene (14%, 10%) in comparison with the corresponding hydrodistillation oils, in addition to α-pinene, β-pinene, sabinene and myrcene, amounting to (9%, 6%), (7%, 4%), (1%, 9%), (1%, 10%) for the leaves and flowers, respectively.

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“…The natural function of glycosidases is to hydrolyze glycosides. Hence, they have been used for the screening of glycosidically bound volatiles in fresh plant material (25,26) and to release aroma compounds (27,28). However, in 1914 Bourquelot already showed that it was possible to use -galactosidase for the reverse reaction by tuning the reaction conditions (29).…”

Section: Preparation Of Glycosidesmentioning

confidence: 99%

Perspectives for the Industrial Enzymatic Production of Glycosides

Roode

Franssen

Padt

et al. 2003

Biotechnology Progress

104

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Glycosides are of commercial interest for industry in general and specifically for the pharmaceutical and food industry. Currently chemical preparation of glycosides will not meet EC food regulations, and therefore chemical preparation of glycosides is not applicable in the food industry. Thus, enzyme-catalyzed reactions are a good alternative. However, until now the low yields obtained by enzymatic methods prevent the production of glycosides on a commercial scale. Therefore, high yields should be established by a combination of optimum reaction conditions and continuous removal of the product. Unfortunately, a bioreactor for the commercial scale production of glycosides is not available. The aim of this article is to discuss the literature with respect to enzymatic production of glycosides and the design of an industrially viable bioreactor system.

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A Simple Method for Screening of Fresh Plant Material for Glycosidic Bound Volatile Compounds¹

Cited by 32 publications

References 4 publications

Volatile constituents of different organs of Psoralea bituminosa L.

Volatile constituents of different organs of Psoralea bituminosa L.

Volatile constituents of the leaves and flowers of Hypericum triquetrifolium Turra

Perspectives for the Industrial Enzymatic Production of Glycosides

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A Simple Method for Screening of Fresh Plant Material for Glycosidic Bound Volatile Compounds1

Cited by 32 publications

References 4 publications

Volatile constituents of different organs of Psoralea bituminosa L.

Volatile constituents of different organs of Psoralea bituminosa L.

Volatile constituents of the leaves and flowers of Hypericum triquetrifolium Turra

Perspectives for the Industrial Enzymatic Production of Glycosides

Contact Info

Product

Resources

About

A Simple Method for Screening of Fresh Plant Material for Glycosidic Bound Volatile Compounds¹