A simple method for screening assessment of skin and eye irritation.

Sekizawa, Jun; Yasuhara, Kazuo; Suyama, Yuji; Yamanaka, Sumie; Tobe, Masuo; Nishimura, Masao

doi:10.2131/jts.19.25

Cited by 32 publications

(25 citation statements)

References 3 publications

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“…In our study, we could also observe that 6% H 2 O 2 alone did not induce irritation or hair loss but in the presence of MEA, toxicity or irritation of H 2 O 2 to skin seems to increase. MEA can induce skin or eye irritation [19,31,32]. It is one of the most frequent occupational allergens in water-based metalworking fluids that can cause inflammation on skin including redness and swelling [33].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide and monoethanolamine are the key causative ingredients for hair dye-induced dermatitis and hair loss

Seo

Bae

Jang

et al. 2012

Journal of Dermatological Science

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Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide and monoethanolamine are the key causative ingredients for hair dye-induced dermatitis and hair loss

Seo

Bae

Jang

et al. 2012

Journal of Dermatological Science

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“…Erythema and edema are scored with grades from 0 to 4 depending on the severity. Histopathological examination should be considered to clarify equivocal responses (10,11). Depending on the severity and reversibility, skin corrosion and irritation is categorized into 1A, 1B, 1C (corrosive), 2 (irritant, mean scores of 2.3~4.0 for erythema or for edema in at least 2 of 3 tested animals) and 3 (mild irritant, mean scores of 1.5~2.3 for erythema or for edema in at least 2 of 3 tested animals) (12).…”

Section: Draize Rabbit Eye and Skin Irritation Testmentioning

confidence: 99%

Alternatives toIn VivoDraize Rabbit Eye and Skin Irritation Tests with a Focus on 3D Reconstructed Human Cornea-Like Epithelium and Epidermis Models

2017

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Human eyes and skin are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular and dermal irritancy for their safe handling and use before release into the market. Draize rabbit eye and skin irritation test developed in 1944, has been a gold standard test which was enlisted as OECD TG 404 and OECD TG 405 but it has been criticized with respect to animal welfare due to invasive and cruel procedure. To replace it, diverse alternatives have been developed: (i) For Draize eye irritation test, organotypic assay, in vitro cytotoxicity-based method, in chemico tests, in silico prediction model, and 3D reconstructed human cornea-like epithelium (RhCE); (ii) For Draize skin irritation test, in vitro cytotoxicity-based cell model, and 3D reconstructed human epidermis models (RhE). Of these, RhCE and RhE models are getting spotlight as a promising alternative with a wide applicability domain covering cosmetics and personal care products. In this review, we overviewed the current alternatives to Draize test with a focus on 3D human epithelium models to provide an insight into advancing and widening their utility.

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“…Primary rabbit skin irritation test: Three Japanese white male rabbit (10 weeks old) were used for rabbit skin irritation test [30]. Fur on neck of the rabbit was removed and cotton pad with 1% of BL58 polymer was applied to the naked skin of the rabbit.…”

Section: Eye Irritancy Testmentioning

confidence: 99%

“…At 0.5 (24 h assessment) and 24 h (48 h assessment) after the test was finished, skin irritancy was megascopically checked [30]. The symptom was assessed in 6 levels: (-), no reaction; (±), light erythematous; (+), erythematous; (2+), and papula; (3+), erythematous, infiltratous, papula, and emphlysis; (4+), bullosum.…”

Section: Primary Human Skin Irritation Testmentioning

confidence: 99%

Structural Analysis and Physiological Properties of Exopolysaccharides Secreted by a Newly Isolated Pseudomonas stutzeri Bl58 under Alkaline Conditions

Yamada¹,

Yoshida²,

Nozaki³

et al. 2016

J Biotechnol Biomater

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We have identified an ethanol-assimilating bacterial strain of Pseudomonas stutzeri that produces copious amounts of an exopolysaccharide when cultured in 5% ethanol under the alkaline condition of pH 10. The objective of this study was to evaluate the potential industrial applications of this exopolysaccharide designated BL58 by characterization of its chemical, rheological and physiological properties. The polymer was purified from the 72 h culture broth with periodic feeding of ethanol and the chemical structure of the polymer was determined by NMR spectroscopy, GC-mass spectrometry and alditol-acetate analysis of methylated hydrolyzates. The analytical data showed the presence of glucose, mannose and 3-O-carboxyethyl rhamnose and suggested that the main chain consists primarily of 1,4-linked glucose and mannose units with unique branching of 3-O-[(R)carboxyethyl] rhamnose at the 3 position of the glucose unit, a structure not previously reported. The polysaccharide has an average molecular weight of 1,800 kDa and the rheological behavior of the aqueous solution from high viscosity sol to viscoelastic gel depended on temperature and pH. Although no overt cytotoxicity was observed, the polymer displayed anti-proliferative activity when tested with leukemia, liver carcinoma and normal pulmonary cells, indicating potential pharmaceutical applications.

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A simple method for screening assessment of skin and eye irritation.

Cited by 32 publications

References 3 publications

Hydrogen peroxide and monoethanolamine are the key causative ingredients for hair dye-induced dermatitis and hair loss

Hydrogen peroxide and monoethanolamine are the key causative ingredients for hair dye-induced dermatitis and hair loss

Alternatives toIn VivoDraize Rabbit Eye and Skin Irritation Tests with a Focus on 3D Reconstructed Human Cornea-Like Epithelium and Epidermis Models

Structural Analysis and Physiological Properties of Exopolysaccharides Secreted by a Newly Isolated Pseudomonas stutzeri Bl58 under Alkaline Conditions

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