A Simple Method for Preparing Thin (10 μm) Histological Sections of Undecalcified Plastic Embedded Bone with Implants

Lübbe, H; Klein, C. P. A. T.; Groot, K. de

doi:10.3109/10520298809107179

Cited by 228 publications

(124 citation statements)

References 3 publications

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“…Nondecalcified thin (10 |xm) sections were made us ing a modified diamond blade sawing microtome technique. 18 The sections were made perpendicular to the long axis of the implant. These sections were stained with methylene blue and basic fuchsine and examined by light microscopy.…”

Section: Histological Proceduresmentioning

confidence: 99%

Effect of calcium phosphate (Ca‐P) coatings on trabecular bone response: A histological study

Caulier

Waerden

Paquay

et al. 1995

J. Biomed. Mater. Res.

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The trabecular bone response to noncoated and fluorapatite (FA), hydroxyapatite (HA), and hydroxyapatite heattreated (HAHT) plasma-sprayed coated implants was in vestigated in a goat animal model. Forty-eight cylindrical implants were inserted into the trabecular bone of the lat eral and medial femoral condyles of twelve goats according to a split plot design. After an implantation period of twelve weeks, the bone-im plant interface was evaluated histologically. Quantitative histomorphometrical measure ments demonstrated a significant difference in bone contact

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Section: Histological Proceduresmentioning

confidence: 99%

Effect of calcium phosphate (Ca‐P) coatings on trabecular bone response: A histological study

Caulier

Waerden

Paquay

et al. 1995

J. Biomed. Mater. Res.

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“…25 Interposition of fibrous tissue between the implant and bone was rarely seen, indicating implant stability and good biocompatibility of both coatings. Fibrous interposition between implant and bone was seen in one SrBG sample at 6 weeks (mean 28.5% of implant surface across the three sections), one SrBG sample at 12 weeks (mean 20.6% of implant surface), and two HA samples at 12 weeks (24.4% and 22.0% of implant surface).…”

Section: Srbg Coating Enhances Early Peri-implant Bone Formationmentioning

confidence: 99%

Enhanced Osseous Implant Fixation with Strontium-Substituted Bioactive Glass Coating

Newman

Lotfibakhshaiesh

O’Donnell

et al. 2014

Tissue Engineering Part A

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The use of endosseous implants is firmly established in skeletal reconstructive surgery, with rapid and permanent fixation of prostheses being a highly desirable feature. Implant coatings composed of hydroxyapatite (HA) have become the standard and have been used with some success in prolonging the time to revision surgery, but aseptic loosening remains a significant issue. The development of a new generation of more biologically active coatings is a promising approach for tackling this problem. Bioactive glasses are an ideal candidate material due to the osteostimulative properties of their dissolution products. However, to date, they have not been formulated with stability to devitrification or thermal expansion coefficients (TECs) that are suitable for stable coating onto metal implants while still retaining their bioactive properties. Here, we present a strontium-substituted bioactive glass (SrBG) implant coating which has been designed to encourage peri-implant bone formation and with a TEC similar to that of HA. The coating can be successfully applied to roughened Ti6Al4V and after implantation into the distal femur and proximal tibia of twenty-seven New Zealand White rabbits for 6, 12, or 24 weeks, it produced no adverse tissue reaction. The glass dissolved over a 6 week period, stimulating enhanced peri-implant bone formation compared with matched HA coated implants in the contralateral limb. Furthermore, superior mechanical fixation was evident in the SrBG group after 24 weeks of implantation. We propose that this coating has the potential to enhance implant fixation in a variety of orthopedic reconstructive surgery applications.

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“…Semi-thin sections (10 pm) were made with a sawing microtome (Leica, Nussloch, Germany) [28]. Only the midsection through the samples was used for histomorphometry.…”

Section: Post-riiorteni Suniple Tiupisition Liistology Rmd L~istoiimentioning

confidence: 99%

Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat

KRUYT¹

2004

Journal of Orthopaedic Research

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Since the application of the autologous bone graft, the need for an alternative has been recognized. Tissue engineering (TE) of bone by combining bone marrow stromal cells (BMSCs) with a porous scaffold, is considered a promising technique. In this study we investigated the potential of tissue engineered bone to heal a critical sized defect in the goat. Orthotopic bone formation was compared to ectopic bone formation in comparable constructs.TE constructs were prepared from goat BMSCs and porous biphasic calcium phosphate ceramic scaffolds. These constructs and scaffolds without cells were implanted paired in critical sized iliac wing defects. Comparable samples were implanted intramuscularly. After 9 ( n = 7) and 12 ( n = 8) weeks implantation, the samples were analyzed histomorphometrically.After 9-weeks implantation in the iliac wing defect, significantly more bone apposition was found in the T E condition. After 12 weeks, the defects were almost completely filled with bone, but no significant advantage of T E was determined anymore. This contrasted with the intramuscular samples where T E implants showed significantly more bone at both time points.In conclusion, bone TE is feasible in critical sized defects. However, when appropriate osteoconductivehnductive materials are applied the effect of cell seeding may be temporary.

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A Simple Method for Preparing Thin (10 μm) Histological Sections of Undecalcified Plastic Embedded Bone with Implants

Cited by 228 publications

References 3 publications

Effect of calcium phosphate (Ca‐P) coatings on trabecular bone response: A histological study

Effect of calcium phosphate (Ca‐P) coatings on trabecular bone response: A histological study

Enhanced Osseous Implant Fixation with Strontium-Substituted Bioactive Glass Coating

Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat

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