A Simple Method for Pineapple Seedling Propagation

Abstract

Pineapple is nutrient and planted in many tropical and subtropical countries. Plenty of elite seedlings were always needed in short time. The common methods for propagating pineapple seedlings have low-multiplying index or take long time. A new method was introduced in this paper. Shoots were induced using stem sections. And then, the shoot bases were cut and cultured on callus-induced medium. The meristematic granules induced were cultured on shoot-induced medium. Shoots can be induced from the meristematic granules. Seedlings can be induced from these shoots in short time. This method overcomes the defects of the common ways. Enough seedlings can be gotten in short time. The operation is simple and easy to learn.

Low-cost of shoot propagation of pineapple (Ananas comosus l. Merr) cv Queen using gandasil-D and AB-mix

“…In previous research, it was reported that in vitro propagation can produce 17.17 explants per culture period, so in a year it is predicted to be able to produce 4,725,264 plantlets/year/explants with uniform plantlet conditions and healthy growth [5]. The use of tissue culture techniques for seedling production in pineapple plants has been widely reported, such as by Shu et al [9], through callus induction using stem parts, the disadvantages of this method are that it takes a long time and low shoot formed. Furthermore, Kodym and Arias [10] produce 3-8 shoots for 12 weeks using MS+BA media, Nelson et al [6] and Supriati [11] produce 2-9 shoots/explants using MS and BAP media, meanwhile Ogero et al [12] using a temporary immersion system that can produce an average of 120-130 shoots/explants.…”