A Simple Method for Isolation of Cardiomyocytes from Adult Rat Heart

Mv, Egorova; Sa, Afanas'ev; Попов, С. В.

doi:10.1007/s10517-005-0492-y

Cited by 19 publications

(9 citation statements)

References 11 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The proportion of these with cardiomyogenic potential is unknown, but the present data indicate that this might be a productive area for further research. Rat hearts contain around 10 7 myocytes [51], and a rough estimate of the infarct volume suggests that in the order of 2-5  10 5 , cells would be susceptible to damage so the number of cells delivered was estimated to be appropriate.…”

Section: Discussionmentioning

confidence: 99%

Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction

McDonald

Mackay‐Sim

Crane

et al. 2010

The Scientific World JOURNAL

View full text Add to dashboard Cite

This study examines the hypothesis that multipotent olfactory mucosal stem cells could provide a basis for the development of autologous cell transplant therapy for the treatment of heart attack. In humans, these cells are easily obtained by simple biopsy. Neural stem cells from the olfactory mucosa are multipotent, with the capacity to differentiate into developmental fates other than neurons and glia, with evidence of cardiomyocyte differentiation in vitro and after transplantation into the chick embryo. Olfactory stem cells were grown from rat olfactory mucosa. These cells are propagated as neurosphere cultures, similar to other neural stem cells. Olfactory neurospheres were grown in vitro, dissociated into single cell suspensions, and transplanted into the infarcted hearts of congeneic rats. Transplanted cells were genetically engineered to express green fluorescent protein (GFP) in order to allow them to be identified after transplantation. Functional assessment was attempted using echocardiography in three groups of rats: control, unoperated; infarct only; infarcted and transplanted. Transplantation of neurosphere-derived cells from adult rat olfactory mucosa appeared to restore heart rate with other trends towards improvement in other measures of ventricular function indicated. Importantly, donor-derived cells engrafted in the transplanted cardiac ventricle and expressed cardiac contractile proteins.

show abstract

Section: Discussionmentioning

confidence: 99%

Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction

McDonald

Mackay‐Sim

Crane

et al. 2010

The Scientific World JOURNAL

View full text Add to dashboard Cite

show abstract

“…Male Wistar Han rats (300-350 g) are used for the experiments in accord with local regulations for laboratory animal use. Ventricular myocytes and right ventricular trabeculae are isolated as previously described (31,32).…”

Section: Methodsmentioning

confidence: 99%

Action potential propagation in transverse-axial tubular system is impaired in heart failure

Sacconi

Ferrantini

Lotti

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

123

View full text Add to dashboard Cite

The plasma membrane of cardiac myocytes presents complex invaginations known as the transverse-axial tubular system (TATS). Despite TATS's crucial role in excitation-contraction coupling and morphological alterations found in pathological settings, TATS's electrical activity has never been directly investigated in remodeled tubular networks. Here we develop an ultrafast random access multiphoton microscope that, in combination with a customly synthesized voltage-sensitive dye, is used to simultaneously measure action potentials (APs) at multiple sites within the sarcolemma with submillisecond temporal and submicrometer spatial resolution in real time. We find that the tight electrical coupling between different sarcolemmal domains is guaranteed only within an intact tubular system. In fact, acute detachment by osmotic shock of most tubules from the surface sarcolemma prevents AP propagation not only in the disconnected tubules, but also in some of the tubules that remain connected with the surface. This indicates that a structural disorganization of the tubular system worsens the electrical coupling between the TATS and the surface. The pathological implications of this finding are investigated in failing hearts. We find that AP propagation into the pathologically remodeled TATS frequently fails and may be followed by local spontaneous electrical activity. Our findings provide insight on the relationship between abnormal TATS and asynchronous calcium release, a major determinant of cardiac contractile dysfunction and arrhythmias.cardiac disease | nonlinear microscopy | voltage imaging | t tubules T he transverse-axial tubular system (TATS) is a complex network characterized by transverse (t tubules, TT) and longitudinal (axial tubules, AT) components running from one transverse tubule to the next (1-3). The TATS of a myocyte rapidly conducts depolarization of the surface sarcolemma (SS) to the core of the cardiomyocyte (4). The coupling between sarcolemmal Ca 2+ entry during an action potential (AP) and Ca 2+ release from sarcoplasmic reticulum (SR) promotes synchronous myofibril activation throughout the myocyte (5). Recent studies highlight that AP-relevant ion channels and transporters are expressed in TATS membrane with different densities and isoforms from those in SS (6, 7). These findings, in combination with diffusional limitations in TATS's lumen (3,8), raise the possibility that AP may differ among membrane domains. Further interest in the TATS AP stems from the recent finding of loss and disorganization of tubules in several pathological conditions, including heart failure (9-14). Because correlation between morphological TATS alterations and Ca 2+ -release asynchronicity has been observed (14, 15), recording AP propagation in TATS can elucidate the fundamental electrophysiological mechanism linking structural and functional anomalies.Although the uniformity of the AP across the whole sarcolemma has been mathematically (16) and experimentally (17) proved, a potential alteration of the AP propagation i...

show abstract

“…The rats were anesthetized, then the left ventricles of heart were rapidly removed in each rat and used the collagenase digestion method that described in references [23,52,63]. [38,64].…”

Section: Apoptosis and Necrosis Analysis By Flowcytometrymentioning

confidence: 99%

Molecular and biochemical evidences on the protective effects of triiodothyronine against phosphine-induced cardiac and mitochondrial toxicity

et al. 2015

View full text Add to dashboard Cite

A Simple Method for Isolation of Cardiomyocytes from Adult Rat Heart

Cited by 19 publications

References 11 publications

Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction

Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction

Action potential propagation in transverse-axial tubular system is impaired in heart failure

Molecular and biochemical evidences on the protective effects of triiodothyronine against phosphine-induced cardiac and mitochondrial toxicity

Contact Info

Product

Resources

About