A Simple Method for Human Whole Blood Microcultures and Its Application in Radiation Biodosimetry

Guo, Yan; Zhu, Wen; Gao, Chao-xian; Hui, Chang-ye; Yang, Xueqin; Yang, Xinyue; Zhimin, Li

doi:10.1097/hp.0000000000000331

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…This method can make us to improve our ability to recognize atypical “dicentrics”, it can ensure the detectability on the critical moment. At the same time, this method can make us from the traditional manual recognition to the automation of recognition 7,13,14,15,16 …”

Section: Discussionmentioning

confidence: 99%

“…At the same time, this method can make us from the traditional manual recognition to the automation of recognition. 7,13,14,15,16 When preparing dose-response curves, Should as far as possible meets the demand of statistics, analysis of cell number formula is based on a chromosome aberration rate in line with the binomial distribution, if known aberration cell rate and the permissible error, can according to the binomial distribution calculated 95% confidence limit formula should count the number of cells, biological experimental error tolerance for general use 15%, it need to count a considerable number of cells, currently use 20% more The formula is: N = [(1 − p) × 96.04]/p. To improve the confidence limit of the results, a sufficient number of cells should be analyzed, and the number of cells to be analyzed depends on the distortion rate.…”

Section: Dose Rate Effects = > Unified Dose-response Calibration Curvementioning

confidence: 99%

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Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Liu

2022

The FASEB Journal

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In order to achieve the goal of rapid response, effective controland protection of life inlarge-scale radiation events, the IAEA Manual 2011 has been revised based on the data of radio-sensitivity, dose-rate findings. Analyze individual differences in radiation sensitivity using 60 Co radiation (0.27 Gy/min). Chromosomal aberrations with different irradiation dose rates were used to establish the biological dose curve and analyze the excess of the "dicentric + ring" caused by the dose rate at each dose point; DAPI-images and Metafer 4 were used to capture metaphase images and make further analysis. The data were collected in 2020, Dicentric + ring/100 Cells was 17.5-43.8, the average value was28.32 ± 6.98. The mean value of Dicentric + ring/100 Cells was 31.37 in males while 25.27 in females, there are significant differences (p < .01). The irradiation dose is dominant, At each dose point, the value of"(dicentric chromosome + centric rings)/cell" is proportional to "dose rate", that is, Y = kx + b, within the dose range of 1-5 Gy, "(dicentric chromosome + centric rings)/ Cell" holds a quadratic linear relationship with dose rate, that is, y = ax 2 + bx + c; The DAPI-images might give you more hints than those of conventional Giemsa-stain.The authors recommend that the IAEA Manual 2011 could be revised based on data of radio-sensitivity and dose-rate, which may contribute to the establishment of a unified dose-response calibration curve and stimulation of potential for automation in cytogenetic biodosimetry. (1) Individual differences of radiosensitivity are very large. (2) At each dose point, "(dicentric chromosome + centric rings)/cell" is proportional to "dose rate", that is, Y = kx + b. (3) "(dicentric chromosome + centric rings)/Cell" is a quadratic linear relationship with dose rate, that is, y = ax 2 + bx + c.(4) We created a "Unity Standard Curve of Biological Dose Estimation". Creating a Unity Standard Curve of Biological Dose, under these circumstances, we can form a joint and rapid response to a nuclear and radiological accident.

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Section: Discussionmentioning

confidence: 99%

Section: Dose Rate Effects = > Unified Dose-response Calibration Curvementioning

confidence: 99%

Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Liu

2022

The FASEB Journal

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“…The SARRP uses a radiation therapy planning (13), verification (14) and delivery system called MuriPlan that allows for both planning and delivering radiation therapy. The MuriPlan planningmodule enables single or multiple beams to be targeted to the isocenter and can create contours of tumors and of organs at risk to reduce dose to normal tissues.…”

Section: Methodsmentioning

confidence: 99%

Stereotactic Body Radiation Therapy Delivery in a Genetically Engineered Mouse Model of Lung Cancer

Lockamy

Zhou

et al. 2016

International Journal of Radiation Oncology*Biology*Physics

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Purpose To implement clinical stereotactic body radiation therapy (SBRT) using a small animal radiation research platform (SARRP) in a genetically engineered mouse model of lung cancer. Methods and Materials A murine model of multinodular Kras-driven spontaneous lung tumors was used for this study. High-resolution cone beam computed tomography (CBCT) imaging was used to identify and target peripheral tumor nodules, whereas off-target lung nodules in the contralateral lung were used as a nonirradiated control. CBCT imaging helps localize tumors, facilitate high-precision irradiation, and monitor tumor growth. SBRT planning, prescription dose, and dose limits to normal tissue followed the guidelines set by RTOG protocols. Pathologic changes in the irradiated tumors were investigated using immunohistochemistry. Results The image guided radiation delivery using the SARRP system effectively localized and treated lung cancer with precision in a genetically engineered mouse model of lung cancer. Immunohistochemical data confirmed the precise delivery of SBRT to the targeted lung nodules. The 60 Gy delivered in 3 weekly fractions markedly reduced the proliferation index, Ki-67, and increased apoptosis per staining for cleaved caspase-3 in irradiated lung nodules. Conclusions It is feasible to use the SARRP platform to perform dosimetric planning and delivery of SBRT in mice with lung cancer. This allows for preclinical studies that provide a rationale for clinical trials involving SBRT, especially when combined with immunotherapeutics.

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A Simple Method for Human Whole Blood Microcultures and Its Application in Radiation Biodosimetry

Cited by 2 publications

References 5 publications

Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Stereotactic Body Radiation Therapy Delivery in a Genetically Engineered Mouse Model of Lung Cancer

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