A simple method for extracting DNA from old skeletal material

Cattaneo, Cristina; Smillie, David; Gelsthorpe, K.; Piccinini, A.; Gelsthorpe, A.R.; Sokol, Robert J.

doi:10.1016/0379-0738(95)01758-b

Cited by 35 publications

(21 citation statements)

References 20 publications

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“…PCR techniques are essentially required for such studies due to the limitations of ancient samples and available amounts of DNA. However, aDNA extraction suitable for successful PCR amplification is complicated by several intrinsic factors: the aDNA is highly degraded and damaged (Pä ä bo, 1989;Hagelberg and Clegg, 1991;Handt et al, 1994;Höss et al, 1996;O'Rourke et al, 1996;Keyser-Tracqui and Ludes, 2005;Mulligan, 2005); presents in low copy number (Handt et al, 1994;O'Rourke et al, 1996;Poinar et al, 1996;Yang et al, 1998;Kumar et al, 2000); it is contaminated with exogenous DNAs that originated from other organisms and contemporary humans (Kolman and Tuross, 2000;Pä ä bo et al, 2004;Calacal and De Ungria, 2005;Kemp and Smith, 2005;Mulligan, 2005;Hunter, 2006); and it coexists with a number of potential PCR inhibitors, such as humic acid, fulvic acid, biologically degraded products, and collagen (Hä nni et al, 1995;Kalmar et al, 2000;Keyser-Tracqui and Ludes, 2005).Because of these complexities, there have been a variety of ancient DNA extraction methods attempted, such as phenol-chloroform extraction (Blake et al, 1992;Kurosaki et al, 1993;Faerman et al, 1995; Hänni et al, 1995) and ethanol or isopropanol precipitation (Kurosaki et al, 1993;Cattaneo et al, 1995Cattaneo et al, , 1997 Hänni et al, 1995) …”

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confidence: 96%

Technical note: Improved ancient DNA purification for PCR using ion‐exchange columns

Kim

Jeon

et al. 2008

American J Phys Anthropol

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A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500-3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples.

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mentioning

confidence: 96%

Technical note: Improved ancient DNA purification for PCR using ion‐exchange columns

Kim

Jeon

et al. 2008

American J Phys Anthropol

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“…[1][2][3][4][5][6][7][8][9] One of the goals of this study was to verify if the procedures of the Institute of Legal Medicine of São Paulo City for cleaning by boiling human remains is a valid and helpful method in the analysis of DNA for identification. The classic morphologic examination of bones is not attempted without removing the decomposing soft tissues by physical or chemical methods.…”

Section: Discussionmentioning

confidence: 99%

A Qualitative Study of Compact Bone Microstructure and Nuclear Short Tandem Repeat Obtained From Femur of Human Remains Found on the Ground and Exhumed 3 Years After Death

Iwamura¹,

Oliveira²,

Soares-Vieira³

et al. 2005

American Journal of Forensic Medicine &Amp; Pathology

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Forensic identification of human remains is composed of anthropological study of race, sex, age, etc. By using these traditional methods, inconclusive or nonidentified cases could be subjected to DNA analysis. However, in spite of advances in human identification techniques, especially by PCR-amplified DNA, some limitations that affect the ability of obtaining DNA from human remains still persist. Light microscope sections of postmortem compact bones from human remains are presented here for the purpose of increasing a forensic examiner's prediction of successful nuclear DNA typing. Femoral compact bones were obtained from 7 human remains found on the ground, in different degrees of decomposition, and were cleaned by boiling to remove soft tissues, 8 collections of bones having undergone natural decomposition, not boiled (as no soft tissue was adhered), and 5 cadavers 12 to 16 hours postmortem. The histologic sections were stained by hematoxylin and eosin, the loci CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317, and amelogenin were amplified by PCR, and the polyacrylamide gel was stained with silver. The results presented here clarify questions concerning the viability of DNA for identification analysis, and they also may help to establish better strategies for optimization of DNA extraction and analysis in compact bones of human remains.

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“…Additionally, silica based DNA binding procedures are known to be effective in removing PCR inhibitors such as humic acid, iron, cobalt, tannins and so forth [1,6,27,[34][35][36].…”

Section: Organic Extraction Versus Silica Column Based Extractionmentioning

confidence: 99%

“…Therefore, chelating methods are routine choices in many laboratories because of the fact that DNA can be successfully isolated of from bones without co-purification of PCR inhibitors [1,[34][35]. Also silica offers many advantages such as low toxicity, biocompatibility, low cost, quick and easy, scalable, efficient removal of PCR inhibitors [27,36].…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted digestion combined with silica-based spin column for DNA isolation from human bones

Ozdemir-Kaynak

Yeşil-Çeliktaş

2015

Analytical Biochemistry

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A simple method for extracting DNA from old skeletal material

Cited by 35 publications

References 20 publications

Technical note: Improved ancient DNA purification for PCR using ion‐exchange columns

Technical note: Improved ancient DNA purification for PCR using ion‐exchange columns

A Qualitative Study of Compact Bone Microstructure and Nuclear Short Tandem Repeat Obtained From Femur of Human Remains Found on the Ground and Exhumed 3 Years After Death

Microwave-assisted digestion combined with silica-based spin column for DNA isolation from human bones

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