A Simple Method for Acquisition and Inoculation of Brassica Yellows Virus from Transgenic Plants and Frozen Infected Leaves by Aphids

Abstract

Brassica yellows virus (BrYV) is a tentative species of the genus Polerovirus, which occurs widely and mostly damage Brassicaceae plants in East Asia. Since BrYV could not be transmitted mechanically, an insect transmission method is required for further virus research. Here, a reliable and unrestricted method was described, in which non-viruliferous aphids (Myzus persicae) acquired BrYV from transgenic Arabidopsis thaliana plants with virus full length genome germinated from seeds and frozen infected leaves were used to transmit the virus to healthy plants, and there was no significant difference in acquisition rate though transmission rate from frozen infected leaves was somehow lower compared to fresh infected leaves. This novel simple method could be applied to preservation of virus inocula, evaluation of variety resistance to BrYV, biological research on interaction among BrYV, aphid and host, which also provide a new idea on establishing a basic method using virus genomic transgenic plants or frozen infected leaves for other poleroviruses research.

Discovery of Brassica Yellows Virus and Porcine Reproductive and Respiratory Syndrome Virus in Diaphorina citri and Changes in Virome Due to Infection with ‘ Ca . L. asiaticus’

“…Whether BrYV has evolved to be transmitted by fungi needs further research. BrYV is transmitted by aphids ( 57 ) and five species, Aphis ( Toxoptera ) aurantii Boyer de Fonscolombe, Aphis ( Toxoptera ) citricidus (Kirkaldy), Aphis gossypii Glover, Aphis spiraecola Patch, and Myzus persicae (Sulzer) (Sternorrhyncha: Aphidae), associated with plant pathogens of citrus in China ( 58 ). Of these, Aphis gossypii is known to feed on Brassica rapa L. (syn.…”