A simple isotopic assay method for human serum phospholipase A<sub>2</sub>activity

Phospholipase A2 has been suggested to be involved in the pathogenesis and pathophysiology of acute pancreatitis. We determined phospholipase A2 and amylase activities in duodenal juice collected during a secretin test from 30 consecutive patients who were suspected to have chronic pancreatitis or biliary disease. The patients underwent endoscopic retrograde cholangiopancreatography (ERCP) the following day. In the 8 patients with ERCP findings of advanced chronic pancreatitis, the mean outputs of phospholipase A2, amylase, and bicarbonate were reduced by 74%, 72%, and 60% compared to the respective values in the 13 (control) patients without a diagnosis of any pancreatic disorder or jaundice. In the 3 patients with recurrent pancreatitis but normal ERCP findings and in the 6 patients with jaundice the output values were not significantly reduced compared to those in the patients without any pancreatic disorder or jaundice. The outputs of amylase and phospholipase A2 were not significantly interrelated, whereas the outputs of phospholipase A2 and bicarbonate correlated well. Receiver characteristic (ROC) curves confirmed the high specificity and sensitivity of phospholipase A2 or bicarbonate output in patients with ERCP findings of advanced chronic pancreatitis compared to those with no changes in pancreatic ducts, with similar probability values of 0.880 +/- 0.111 (SEM), compared to the respective lower value of amylase, 0.676 +/- 0.118. Phospholipase A2 and bicarbonate output proved of equal value as markers of chronic pancreatitis and were superior to amylase output in the secretin test.

show abstract

Duodenal secretion of phospholipase A 2 , amylase, and bicarbonate in chronic pancreatitis

Mäkelä

Kuusi

Seppälä

et al. 1998

Journal of Gastroenterology

Self Cite

View full text Add to dashboard Cite

show abstract

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Kaiser

1999

Critical Reviews in Clinical Laboratory Sciences

View full text Add to dashboard Cite

Phospholipase A2 (PLA2) is an enzyme that catalyzes the hydrolysis of membrane phospholipids. This article reviews the source and structure of PLA2, the involvement of the enzyme in various biological and pathological phenomena, and the usefulness of PLA2 assays in laboratory diagnostics. Of particular importance is the role of PLA2 in the cellular production of mediators of inflammatory response to various stimuli. Assays for PLA2 activity and mass concentration are discussed, and the results of enzyme determinations in plasma from patients with different pathological conditions are presented. The determination of activity and mass concentration in plasma is particularly useful in the diagnosis and prognosis of pancreatitis, multiple organ failure, septic shock, and rheumatoid arthritis. A very important result is the demonstration that PLA2 is an acute phase protein, like CRP. Indeed, there is a close correlation between PLA2 mass concentration and CRP levels in several pathological conditions. Although the determination of C-reactive protein is much easier to perform and is routinely carried out in most clinical laboratories, the assessment of PLA2 activity or mass concentration has to be considered as a reliable approach to obtain a deeper understanding of some pathological conditions and may offer additional information concerning the prognosis of several disorders.

show abstract

The Usefulness of Laboratory Tests in the Early Assessment of Severity of Acute Pancreatitis

Rettally

Skarda

Garza

et al. 2003

Critical Reviews in Clinical Laboratory Sciences

View full text Add to dashboard Cite

Acute pancreatitis is a disorder that affects approximately 200,000 individuals in the U.S. annually. While most cases are mild, up to 30% of patients will have a complicated course with prolonged hospitalization and significant morbidity and mortality. Early institution of several therapeutic interventions, such as enteral nutrition, prophylactic antibiotics, endoscopic retrograde cholangiopancreatography (ERCP) and intensive care monitoring, have been shown to decrease the morbidity associated with severe acute pancreatitis. However, the ability of clinicians to predict, upon presentation, which patient will have mild or severe pancreatitis has remained poor over the years, thus leading to a delay in the institution of such treatments. Researchers have focused on markers that might improve upon clinical prediction alone. While data have shown the predictive value of tools such as Ranson's and Glasgow's criteria, C-reactive protein (CRP) and the APACHE score, their application in clinical practice has been limited by a time delay of at least 48 h in the former two and by being cumbersome in the latter. Thus, our focus is to critically appraise the evidence available for various biochemical markers in their ability to distinguish mild and severe acute pancreatitis early and more accurately than currently available tools.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A simple isotopic assay method for human serum phospholipase A₂activity

Cited by 5 publications

References 15 publications

Duodenal secretion of phospholipase A 2 , amylase, and bicarbonate in chronic pancreatitis

Duodenal secretion of phospholipase A 2 , amylase, and bicarbonate in chronic pancreatitis

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

The Usefulness of Laboratory Tests in the Early Assessment of Severity of Acute Pancreatitis

Contact Info

Product

Resources

About

A simple isotopic assay method for human serum phospholipase A2activity

Cited by 5 publications

References 15 publications

Duodenal secretion of phospholipase A 2 , amylase, and bicarbonate in chronic pancreatitis

Duodenal secretion of phospholipase A 2 , amylase, and bicarbonate in chronic pancreatitis

Phospholipase A2: Its Usefulness in Laboratory Diagnostics

The Usefulness of Laboratory Tests in the Early Assessment of Severity of Acute Pancreatitis

Contact Info

Product

Resources

About

A simple isotopic assay method for human serum phospholipase A₂activity

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics