A simple immunofluorescent technique for the detection of human rotavirus

Moosai, R. B.; Gardner, P. S.; Almeida, June D.; Greenaway, M. A.

doi:10.1002/jmv.1890030304

Cited by 18 publications

(4 citation statements)

References 12 publications

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“…In this study, we developed and compared several rotavirus detection methods. IF was useful for the detection of simian and human rotaviruses, in agreement with the literature (5,10,14,18). Of the ELISAs tested, the commercially available Enzygnost assay was the most sensitive.…”

Section: Resultssupporting

confidence: 89%

Comparison of methods for rotavirus detection in water and results of a survey of Jerusalem wastewater

Guttman-Bass¹,

Tchorsh²,

Marva³

1987

Appl Environ Microbiol

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Methods for the detection of viable rotaviruses and rotavirus antigen in water were developed and compared. The methods included laboratory-developed enzyme-linked immunosorbent assays (ELISAs) with chromogenic and luminescent substrates, commercial Rotazyme and Enzygnost ELISAs, and an indirect immunofluorescent assay. Of the methods tested, the immunofluorescent assay and the Enzygnost ELISA were the most sensitive for the simian rotavirus SA-li. All of the methods were positive for human rotavirus from clinical specimens. Seeded SA-li rotavirus was concentrated from water by adsorption to and elution from Zeta Plus ifiters followed by organic flocculation. Interference with the assays by components of the wastewater concentrates was minimal for the ELISAs, although the undiluted organic flocs were cytotoxic for the immunofluorescent assay. A survey of Jerusalem wastewater was carried out over the course of 1 year, and samples were assayed for rotaviruses and enteroviruses. Although enteroviruses were found in almost all of the samples, all samples were negative for rotaviruses. The concentration of rotaviruses in the wastewater was thus below the detection limit of the methods used.

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Section: Resultssupporting

confidence: 89%

Comparison of methods for rotavirus detection in water and results of a survey of Jerusalem wastewater

Guttman-Bass¹,

Tchorsh²,

Marva³

1987

Appl Environ Microbiol

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“…Negative staining is suitable for this purpose, and aggregation of the virions with antiserum (immune-electron microscopy) facilitates their detection and specific identification. A cell-culture method has been described (17) in which trypsintreated fecal extracts, containing rotavirus, produced antigen in cell monolayers that was detectable by immunofluorescence, although progressive infection did not occur. Recently, ELISA for human-rotavirus antigen in stool suspensions has been described (28).…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Human rotavirus gastroenteritis

Bowdre

1980

Clinical Microbiology Newsletter

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“…using standardized techniques (Moosai et al 1979). The bovine anti-rotavirus antiserum described in this report was employed at a dilution of I in 2oo, followed by FITC conjugated rabbit anti-bovine Ig antiserum (Wellcome Reagents).…”

Section: Reverse Passive Haemagglutination (Rph)mentioning

confidence: 99%

A Solid-Phase System (SPACE) for the Detection and Quantification of Rotavirus in Faeces

Bradburne

Almeida

Gardner

et al. 1979

Journal of General Virology

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SUMMARYThis report describes the development of a solid-phase haemadsorption system using chromic chloride-linked, antibody coated erythrocytes. It is proposed to call this technique solid phase aggregation of coupled erythrocytes (SPACE). The system is suitable for the detection of virus antigens, such as from rotavirus infections, which are present in 'dirty' or 'mixed' preparations such as faeces, urine or exudates. The test uses microtitre U-form plates coated" with specific antivirus antibody; faecal suspensions are added and virus or antigen allowed to adsorb. The plates are then washed and adsorbed antigens are detected by the addition of virus-specific IgG-coated erythrocytes. The resultant settling pattern is read in the same manner as a conventional haemagglutination test. The system is compared with electron microscopy and fluorescent antibody techniques.

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A simple immunofluorescent technique for the detection of human rotavirus

Cited by 18 publications

References 12 publications

Comparison of methods for rotavirus detection in water and results of a survey of Jerusalem wastewater

Comparison of methods for rotavirus detection in water and results of a survey of Jerusalem wastewater

Human rotavirus gastroenteritis

A Solid-Phase System (SPACE) for the Detection and Quantification of Rotavirus in Faeces

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