2022
DOI: 10.1186/s12951-022-01256-5
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A simple immunoassay for extracellular vesicle liquid biopsy in microliters of non-processed plasma

Abstract: Background Extracellular vesicles (EVs), released by most cell types, provide an excellent source of biomarkers in biological fluids. However, in order to perform validation studies and screenings of patient samples, it is still necessary to develop general techniques permitting rapid handling of small amounts of biological samples from large numbers of donors. Results Here we describe a method that, using just a few microliters of patient’s plasma… Show more

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Cited by 6 publications
(2 citation statements)
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“…As expected, levels of tetraspanins varied among different individuals, either patients with cancer or another condition. In any case, cancer patients presented, in general, higher CD63 + -CD81 + signal than non-cancer donors, in line with previous observations in lung cancer patients compared with healthy donor plasma ( Campos-Silva et al, 2022a ). Then, we compared EV-released MICA (combining MICA capture with tetraspanin detection) with soluble released ligands.…”
Section: Resultssupporting
confidence: 91%
See 1 more Smart Citation
“…As expected, levels of tetraspanins varied among different individuals, either patients with cancer or another condition. In any case, cancer patients presented, in general, higher CD63 + -CD81 + signal than non-cancer donors, in line with previous observations in lung cancer patients compared with healthy donor plasma ( Campos-Silva et al, 2022a ). Then, we compared EV-released MICA (combining MICA capture with tetraspanin detection) with soluble released ligands.…”
Section: Resultssupporting
confidence: 91%
“…Next, we compared data of soluble NKG2D-ligands with EV-released. Importantly, in order to minimize EV subpopulation selection during sample processing for EV enrichment and to encourage easy translation in clinical settings in the future, direct detection by sandwich ELISA was performed ( Campos-Silva et al, 2021 ; Campos-Silva et al, 2022a ). To ensure the association of NKG2D-L to EVs, different combinations of capture and detection antibodies were used as depicted.…”
Section: Resultsmentioning
confidence: 99%