A simple highly selective and sensitive hydroquinone-based two-photon fluorescent probe for imaging peroxynitrite in live cells

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Currently, the fluorescent probe is an important method for detecting heavy metal ions, especially mercury ion (Hg ), which is harmful to the health of humans and the environment due to its toxicity and extensive use. In this paper, we designed and synthesized a colorimetric and long-wavelength fluorescent probe Hg-P with high sensitivity and excellent selectivity, which could detect Hg by the changes of visual color, fluorescence and absorption spectroscopy. With the addition of Hg to probe Hg-P solution, its color changed from yellow to pink, and showed a 171 nm red-shifted absorption spectrum. Probe Hg-P was used in real water and soil solution samples to detect Hg , and the result is satisfactory. Therefore, this new probe shows great value and application in detecting Hg in the environment.

Section: Introductionmentioning

confidence: 99%

A highly selective colorimetric and long‐wavelength fluorescent probe for the detection of Hg²⁺

Wang

Liu

et al. 2018

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“…As important factors for an excellent probe, selectivity, and anti‐interference were also studied . As seen in Figure a, there were no fluorescence intensity changes with the addition of Br − , I − , Cl − , F − , CO 3 2− , NO 3 − , PO 4 2− , SO 4 2− , HPO 4 2− , urea, Fe 2+ , Ca 2+ , K + , Mg 2+ , Mn 2+ , and Na + .…”

Section: Resultsmentioning

confidence: 98%

A colorimetric fluorescent probe for rapid and specific detection of nitrite

Shi

Yang

et al. 2019

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The method of fluorescent probes has been an important technique for detection of nitrite (NO2−). As an important inorganic salt, excessive nitrite would threaten humans and the environment. In this paper, a colorimetric fluorescent probe P‐N (1,2‐diaminoanthraquinone) with rapid response and high selectivity, which could detect NO2− by visual colour changes and fluorescence spectroscopy is presented. The probe P‐N solution (pH 1) changed from pink to colourless with the addition of NO2− and fluorescence intensity at 639 nm clearly decreased. Good linear exists between fluorescence intensities and NO2− concentrations for the range 0–16 μM, and the detection limit was 54 nM (based on a 3σ/slope). Moreover, probe P‐N could also detect NO2− in real water samples, and results were all satisfactory. Probe P‐N shows great practical application value for detecting NO2− in the environment.

“…A human cervical carcinoma cell line (HeLa) was obtained from the Hebei University, China. HeLa cells were maintained in Dulbecco' modified Eagle' medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C under an humidified atmosphere in a 5% CO 2 /95% air incubator (SANYO MCO‐15A) . HeLa cells were seeded into 6‐well plates and allowed to attach for 24 h. It was essential to wash cells three times with phosphate‐buffered saline (PBS) to remove metabolic waste products.…”

Section: Methodsmentioning

confidence: 99%

A triazole‐based fluorescence probe for detecting Hg²⁺ ions and its biological application

Liu

Ding

et al. 2019

A new compound, ethyl 5-phenyl-2-(p-tolyl)-2H-1,2,3-triazole-4-carboxylate was successfully introduced and synthesized as a novel rhodamine B derivative named REPPC, and characterized by 1 H nuclear magnetic resonance (NMR), 13 C NMR, and high resolution mass spectrometry (HRMS). It showed an obvious fluorescence and UV-visible light absorption enhancement towards Hg 2+ ion without interference from common metal ions in N,N-dimethylformamide-H 2 O (pH 7.4). The spirolactam ring moiety of rhodamine in REPPC was converted to the open-ring form generating a 1:1 complex with the intervention of a mercury ion, verified by electrospray ionization-mass spectroscopy testing and density functional theory calculation.REPPC was used to visualize the level of mercury ions in living HeLa cells with encouraging results. KEYWORDSbinding mechanism, bio-imaging, ethyl 5-phenyl-2-(p-tolyl)-2H-1,2,3-triazole-4-carboxylate, mercury ion As shown in Scheme 1, first rhodamine B hydrazide 1 was synthesized according to a reported procedure. [15] Next, 20 ml of ethanol was used SCHEME 1 Synthesis route of REPPC. 130LIU ET AL. 0 3 shows the corresponding colour photograph for REPPC in the presence of different metal ions. There was a 615-fold increase in fluorescence signal at 577 nm for the REPPC-Hg 2+ system; this result suggested that REPPC can be used as a sensor to detect Hg 2+ using FIGURE 1 Effect of pH on the fluorescence signal of REPPC and the REPPC-mercury complex 132 LIU ET AL.