A Simple Fluorescent Probe Derived from Naphthylamine for Selective Detection of Hg^II, Fe^II and Fe^III Ions in Mixed Aqueous Media: Applications in Living Cells and Logic Gates

Abstract

A naphthylamine‐based probe was utilized for the detection of HgII, FeII and FeIII in mixed aqueous media by fluorescence quenching. These sensitive metal ions bind with the probe by forming a 1:1 complex. The time‐resolved fluorescence and quantum yield of the probe in the absence as well as in the presence of metal ions were investigated. The extent of binding of the probe with sensitive metal ions was calculated. The sensitivity of the probe in the presence of other metal ions was examined using competitive binding studies. The probe displayed sensitivity towards HgII during in vitro as well as in vivo studies. This multianalyte probe has potential biological applications in cell imaging and in logic gates.Correspondence that discusses a caveat with regard to these studies is available: DOI 10.1002/ejic.201500566

Highly Sensitive Coumarin–Pyrazolone Probe for the Detection of Cr3+ and the Application in Living Cells

“…The obtained emission quenching was almost ∼98%, and fluorescence changes were caused by the chelation-enhanced quenching (CHEQ) pathway. These results are consistent with previous reports . The calibration plot was plotted between the relative emission intensity and concentrations of Cr 3+ ions, and it shows a good linear relationship (Figure ) in the concentration range from 5 to 75 nM.…”

“…These results are consistent with previous reports. 34 The calibration plot was plotted between the relative emission intensity and concentrations of Cr 3+ ions, and it shows a good linear relationship (Figure 3) in the concentration range from 5 to 75 nM. Based on the emission quenching experiments, the lowest limit of detection for Cr 3+ ions is calculated as 37 pM.…”

A Coumarin-Based Fluorescence Probe for Selective Recognition of Cu2+ Ions and Live Cell Imaging

“…By the virtue of these distinct advantages, fluorescence bioimaging technology has been applied successfully in living cells [16][17][18]. Typical fluorescent sensors have been documented involving inorganic composites, such as quantum dots @MOFs, or organic fluorophores such as photochromic diarylethene derivatives, coumarin derivatives, rhodamine B-based fluorescent probes, and naphthalimiderhodamine B derivative [19][20][21][22][23][24][25][26][27]. Although a variety of fluorescent probes for the recognition of Cu 2+ ions have been reported, it is still a challenge to directly contact metal ions with fluorophore for signal transduction.…”

Key Considerations for Sensing FeII and FeIII in Aqueous Media

“…A re‐examination of UV/Vis data in Figure 2 in ref 1. provides a more probable interpretation of the data.…”

“…Recently Ghosh et al reported on the fluorescent response of commercially available N ‐(2‐aminoethyl)naphthalen‐1‐amine toward Fe II , Fe III , and Hg II 1. The authors conclude that metal binding to the diamine chelator leads to chelation‐induced quenching; however, the experimental protocols are incompatible with the aqueous chemistry of Fe III , which precludes this interpretation of the data.…”