Abstract:High-throughput western blot (WB) analysis of small and precious samples, including various age-related subtype-specific human induced neurons (hiNs), endows the ability to obtain more consistent, comparable, and informative data from materials with highly limited availability. In this study, p-toluenesulphonic acid (PTSA), an odorless tissue fixative, was used to inactive HRP for developing a high-throughput WB method. PTSA-treated blots showed fast and efficient inactivation of HRP without detectable protein… Show more
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