A simple, fast and reliable liquid chromatographic method for the determination of theophylline in urine, saliva and plasma samples

Charehsaz, Mohammad; Şahin, Gönül; Gürbay, Aylin; Aydın, Ahmet

doi:10.1016/j.toxlet.2011.05.685

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…The dominant peak (peak 1, 415.481 m/z) in the laser desorption ionisation‐mass spectrometry spectrum from Acuvue Oasys surface after the uptake study was therefore assigned to dexamethasone, showing that dexamethasone was absorbed on the Acuvue Oasys lens surface. Peaks 2 (332.108 m/z) and 3 (304.251 m/z) in the Isopto‐Maxidex spectrum are characteristic for benzalkonium chloride, where (304.251 m/z) belongs to C 12 ‐benzalkonium chloride (n = 12) and (332.108 m/z) to C 14 ‐benzalkonium chloride (n = 14) . The characteristic peaks of benzalkonium chloride can also be observed in the laser desorption ionisation‐mass spectrometry spectrum of Acuvue Oasys as peaks 2 (332.772 m/z) and 3 (304.120 m/z), revealing an uptake of benzalkonium chloride on the contact lens as well.…”

Section: Resultsmentioning

confidence: 98%

In vitro and in vivo effects of ophthalmic solutions on silicone hydrogel bandage lens material Senofilcon A

Erdal

Adolfsson

Lima

et al. 2018

Clinical and Experimental Optometry

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Section: Resultsmentioning

confidence: 98%

In vitro and in vivo effects of ophthalmic solutions on silicone hydrogel bandage lens material Senofilcon A

Erdal

Adolfsson

Lima

et al. 2018

Clinical and Experimental Optometry

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“…As discussed in the Introduction, in the literature we either find HPLC methods for quantification of paracetamol or theophylline in simple matrixes, or in human milk, as residues. Some works do not state retention times at all [16] , [19] , [20] , [21] , [22] , [23] and others report shorter (between 5 and 6.6 min) retention times for theophylline [14] , [15] , [18] . Paracetamol and theophylline residues, quantified in breast milk, presented retention times of 9 and 18 min [17] , respectively, which are comparable to our work (paracetamol), or much higher (theophylline).…”

Section: Resultsmentioning

confidence: 99%

“…There are several published methods for determination of theophylline or paracetamol individually, or in combination with other drugs, in different matrices, but there is a lack of similar studies involving quantification of paracetamol or theophylline in powdered or fresh milk and none, to our knowledge, involves quantification of these drugs in milk-based dosage forms. Just to name a few works, theophylline was quantified by high-performance liquid chromatography (HPLC) in urine, saliva and plasma samples [14] alone and, in combination with etophylline, in human plasma [15] , breast milk [16] , [17] and tablets [18] ; paracetamol was quantified by UV–Vis and HPLC in tablets [19] , [20] , [21] , [22] and biological fluids [23] . Reports of HPLC drug determinations as residues in breast milk were found for several drugs [24] , including paracetamol and theophylline [17] , but those methods require extensive pretreatment of samples to remove matrix interference.…”

Section: Introductionmentioning

confidence: 99%

Quantification of theophylline or paracetamol in milk matrices by high-performance liquid chromatography

Fernandes

Aguiar

Fernandes

et al. 2017

Journal of Pharmaceutical Analysis

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A simple, accurate and sensitive high-performance liquid chromatography (HPLC) method was developed, validated and applied to the determination of either theophylline or paracetamol in milk-based samples. The method allowed drug quantification in fresh and powdered milk with a relatively short run time of analysis and it was also successfully applied to the quantification of the drugs in solid dosage forms intended for pediatric use. Moreover, the main significant advantages over other published works are the simplicity of the sample preparation, reduced assay time and sample loss. The method meets the International Conference on Harmonization guideline for analytical methods validation regarding specificity, linearity, accuracy, precision, specificity and robustness as required by health authorities and applied by industry while designing and marketing new drug products. The technique encompasses the separation of the analytes with a reverse phase C18 column under isocratic conditions and UV detection at 272 nm and 243 nm, respectively, for theophylline and paracetamol. The lower limit of quantification for both drugs was determined as 0.2 µg/mL and the between-batch accuracy was approximately 99.7%. This HPLC method allows quantification of theophylline and paracetamol in milk matrices and it can be applied in the design, development and production of milk-based pediatric dosage forms.

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“…CKX31) was used to confirm the formation of Nile Tilapia fish scale-based collagen nanoparticles 57 . We evaluated the encapsulation efficiency (EE) of theophylline according to Charehsaz et al, 2014 by spectrophotometry (Spectrostar Nano , BMG Labtech) 58 and calculated its percentage using the below formula 59 : W P : the total amount of the encapsulated purified theophylline, W t : the total quantity of the encapsulated theophylline during the preparation.…”

Section: Methodsmentioning

confidence: 99%

Theophylline-encapsulated Nile Tilapia fish scale-based collagen nanoparticles effectively target the lungs of male Sprague–Dawley rats

Anwar

Shalaby

Saeed

et al. 2022

Sci Rep

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Nile Tilapia fish scale collagen has high biodegradability, excellent biocompatibility, and low antigenicity. We assessed both the encapsulation efficiency of theophylline into Nile Tilapia fish scale-based collagen nanoparticles and their stability as a pulmonary drug delivery system in male Sprague–Dawley rats. The present study has demonstrated the successful encapsulation of theophylline into the synthesised nanoparticles as shown by spectrophotometric analysis, light microscope, scanning electron microscope, transmission electron microscope, and dynamic light scattering. The antibacterial activity of the nanoparticles improves with increasing their concentrations. Intratracheal treatment of rats using theophylline-encapsulated nanoparticles reduced the levels of creatinine, alanine transaminase, and aspartate transaminase, compared to the control group. Nevertheless, nanoparticles combined with theophylline exhibited no effects on cholesterol and triglycerides levels. Histopathological examination revealed typical uniform and diffuse thickening of the alveolar walls with capillary oedema in treated rats. We concluded that the synthesised collagen nanoparticles appropriately target the lungs of male Sprague–Dawley rats when delivered via a nebuliser, showing good tolerability to lung cells. However, dose ratio of collagen nanoparticles to theophylline needs further evaluation. The nanoprecipitation method may be optimised to involve poorly water-soluble inhaled drugs, and avoid the drawbacks of traditional drug delivery.

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A simple, fast and reliable liquid chromatographic method for the determination of theophylline in urine, saliva and plasma samples

Cited by 4 publications

References 13 publications

In vitro and in vivo effects of ophthalmic solutions on silicone hydrogel bandage lens material Senofilcon A

In vitro and in vivo effects of ophthalmic solutions on silicone hydrogel bandage lens material Senofilcon A

Quantification of theophylline or paracetamol in milk matrices by high-performance liquid chromatography

Theophylline-encapsulated Nile Tilapia fish scale-based collagen nanoparticles effectively target the lungs of male Sprague–Dawley rats

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