A simple, fast and cheap isolation and multiplication technique of Phytophthora infestans
Monica do Rocio Andrade,
Aline José Maia,
Stephani Sloboda
et al.
Abstract:In this study, we present a method for isolation and propagation of Phytophthora infestans, a challenging-to-isolate phytopathogenic oomycete. The procedure initiated with naturally infected potato leaves, which underwent a 30-minute sanitization under running water. Subsequently, healthy potato tubers were meticulously washed with a neutral detergent, sterilized using alcohol, and then flamed. Slices 4 mm thick were carefully cut out of the potato tubers. Slices were used to cover 2 x 4 mm sterilized leaf pie… Show more
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