A Simple Extraction Method of Soluble Carbohydrates from Various Organs of Several Ornamental Plants Using a Vacuum Manifold

Norikoshi, Ryo; Kato, Mayumi; Ichimura, Kazuo

doi:10.2525/ecb.46.49

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…We determined the soluble carbohydrate contents of the petals following the method of Norikoshi et al (2008). We collected petals from the middle florets of all 10 cultivars on day 0, and from 'Port Light Pair Beauty' and 'Moon Waltz' flowers that had been treated with CMIT/MIT or Glc + CMIT/MIT on days 0, 2, 4, and 6.…”

Section: Soluble Carbohydrate Contents Of the Petalsmentioning

confidence: 99%

Effects of Bacterial Proliferation and Soluble Carbohydrate Levels on the Vase Life of Cut Dahlia (Dahlia variabilis) Flowers

2019

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Cut dahlia (Dahlia variabilis) flowers have recently become popular in Japan, but have the disadvantage of only having a short vase life. Here, we sought to clarify which factors are responsible for this by investigating the effects of an antibacterial (CMIT/MIT) treatment and a combined glucose plus antibacterial (Glc + CMIT/ MIT) treatment on the vase life of the cut flowers of 10 dahlia cultivars, as well as the bacterial growth kinetics in their vase solutions and the soluble carbohydrate contents of their petals. We found that the CMIT/MIT treatment extended the vase life of 'Kamakura', 'Magic Pink' and 'Purple Stone', all of which had relatively high numbers of bacteria in their vase solutions. By contrast, the Glc + CMIT/MIT treatment significantly extended the vase life of three cultivars and also increased the fresh weight of nine cultivars. A comparison of two cultivars with relatively long and short vase lives ('Moon Waltz' and 'Port Light Pair Beauty', respectively) showed that a longer vase life was related to a higher carbohydrate content in the petals. Together, these findings suggest that maintaining the carbohydrate level is important for extending the vase life of cut dahlia flowers.

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Section: Soluble Carbohydrate Contents Of the Petalsmentioning

confidence: 99%

Effects of Bacterial Proliferation and Soluble Carbohydrate Levels on the Vase Life of Cut Dahlia (Dahlia variabilis) Flowers

2019

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“…Soluble carbohydrates were extracted following the methods of Norikoshi et al (2008). In brief, the upper petal lobes were immersed in 80% ethanol at 70°C for 20 min.…”

Section: Determination Of Soluble Carbohydratesmentioning

confidence: 99%

“…The identity of each peak was confirmed with authentic carbohydrates. The peak areas were determined by an integrator and the amounts of each carbohydrate in each sample were calculated as described by Norikoshi et al (2008).…”

Section: Determination Of Soluble Carbohydratesmentioning

confidence: 99%

Effects of Pulse Treatments with Sucrose, Silver Thiosulfate Complex (STS) and Calcium Chloride on the Vase Life and Soluble Carbohydrate and Aurone Levels in Cut Snapdragon Flowers

2022

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The effects of pulse treatments with 0.2 mM silver thiosulfate complex (STS), 5 and 10% sucrose, and 0.2% calcium chloride (CaCl 2 ) and their combinations on the vase life and soluble carbohydrate and aurone levels in cut snapdragon (Antirrhinum majus) flowers were investigated. In the pulse treatment, the cut flowers were treated with these chemical solutions for 24 h before being transferred to distilled water. The uptake of solution for pulse treatments was decreased in the treatments with STS, sucrose, and CaCl 2 and the combined STS and sucrose treatment further suppressed uptake. The sucrose treatment significantly increased the number of open flowers and extended the vase life, with no marked differences in these effects between the 5% and 10% sucrose solutions. The STS treatment slightly promoted flower opening but did not significantly extend vase life. The CaCl 2 treatment did not affect flower opening or vase life. The relative fresh weight (RFW) and water uptake of cut flowers were increased in the sucrose treatments and were only slightly affected by the STS and CaCl 2 treatments. The petal aurone levels were significantly higher in the sucrose treatments, particularly at 10% sucrose. The glucose, fructose, and sucrose petal contents were generally higher in the sucrose treatments than in the treatments that did not include sucrose. The ethylene production of flowers was relatively low at harvest and somewhat increased during senescence. Therefore, the infectiveness of STS on vase life may be due to low ethylene production. We conclude that pulse treatments with solutions containing sucrose increase the sugar contents and aurone levels in petals, as well as improve the vase life of cut snapdragons.

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“…The chemical structure of the purified compound was analyzed by CI-MS and ESIMS using a JMS-SX 102A instrument (Jeol, Tokyo, Japan) and Determination of 2-CEIX contents in petals, the other floral parts, stem, and leaves 2-CEIX was extracted based on the conventional method for soluble carbohydrate extraction as reported by Norikoshi et al (2009). Briefly, petals, the other floral parts that included stamens, pistil and calyx, stem and leaves (1 g FW) obtained from 3 plants was extracted with 80% ethanol, and purified using the SepPak C18.…”

Section: Isolation and Identification Of Compoundmentioning

confidence: 99%

“…The column was kept at 80°C, and eluted with water at a flow rate of 0.8 mL·min −1 . The contents of 2-CEIX were calculated as described in Norikoshi et al (2009).…”

Section: Isolation and Identification Of Compoundmentioning

confidence: 99%

2-Cyanoethyl-isoxazolin-5-one Is a Major Low Molecular Weight Nitrogenous Compound in Sweet Pea (Lathyrus odoratus L.)

et al. 2016

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An unidentified compound was detected in sweet pea (Lathyrus odoratus L. ʻDianaʼ) petals by HPLC analysis using a cation-exchange column for soluble carbohydrate analysis. This compound was identified as 2-cyanoethyl-isoxazolin-5-one (2-CEIX) using 1 H-NMR, 13C-NMR, and CI-MS and ESIMS. 2-CEIX was detected in the petals, leaves and stem. Amino acid and other nitrogenous compound contents in these organs were compared with 2-CEIX. The content of asparagine was highest, followed by 2-CEIX in the petals, and 2-CEIX was highest among nitrogenous compounds in the stem and leaves. The 2-CEIX content in the petal decreased during flower opening, but those in the petals and the other floral parts increased during senescence regardless of sucrose treatment. These trends differed from those of monosaccharides, sucrose and cyclitols. Thus, the role of 2-CEIX appears to differ from those of soluble carbohydrates. 2-CEIX was not detected in phloem and xylem saps. The results suggest that 2-CEIX is a major nitrogenous compound of low molecular weight and is likely to be produced in situ in various organs in sweet pea.

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A Simple Extraction Method of Soluble Carbohydrates from Various Organs of Several Ornamental Plants Using a Vacuum Manifold

Cited by 6 publications

References 16 publications

Effects of Bacterial Proliferation and Soluble Carbohydrate Levels on the Vase Life of Cut Dahlia (<i>Dahlia variabilis</i>) Flowers

Effects of Bacterial Proliferation and Soluble Carbohydrate Levels on the Vase Life of Cut Dahlia (<i>Dahlia variabilis</i>) Flowers

Effects of Pulse Treatments with Sucrose, Silver Thiosulfate Complex (STS) and Calcium Chloride on the Vase Life and Soluble Carbohydrate and Aurone Levels in Cut Snapdragon Flowers

2-Cyanoethyl-isoxazolin-5-one Is a Major Low Molecular Weight Nitrogenous Compound in Sweet Pea (<i>Lathyrus odoratus</i> L.)

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