A Simple Device for Measuring Diameters of Fibrinolysis Zones on Fibrin Plates

Haverkate, F.

doi:10.1159/000213739

Cited by 5 publications

(4 citation statements)

References 4 publications

(5 reference statements)

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“…From each euglobulin fraction, 3 drops of 30 pi each were placed on the different fibrin plates. After incubation at 37 °C for 18 h, two perpendicular diameters of each lysed zone were measured with H averkate's [11] special device. The mean diameters of the lysed zones were used to represent the fibrinolytic activity of the euglobulin fraction.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of the Fibrinolytic System in Behçet’s Disease?

Asbeck¹,

Meyer-Boernecke²,

Loo³

1977

Pathophysiol Haemos Thromb

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Suppression of the fibrinolytic system is a well-known phenomenon in patients with Behçet’s disease. It is generally explained by an increase in the inhibitory potential. In order to prove this theory, the fibrinolytic system was investigated in 5 male patients with severe chronic Behçet’s disease. By the venous occlusion test, a marked suppression of the activatability of the system could be demonstrated (euglobulin lysis time, various fibrin plate assays). Results of analysis of the known inhibitors could not explain the phenomenon: antithrombin III, α₂-macroglobulin, fast-reacting α₂-antiplasmin, C1 inactivator, and the plasmin-antiplasmin complex were normal. The only change was an elevation of α₁-antitrypsin and fibrinogen in 4 patients possibly due to an acute phase reaction. In addition to the possibility of a hitherto unidentified inhibitor, therefore, a decrease in production or in release of vessel wall activators must also be considered in the pathogenesis of this disorder.

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Section: Methodsmentioning

confidence: 99%

Inhibition of the Fibrinolytic System in Behçet’s Disease?

Asbeck¹,

Meyer-Boernecke²,

Loo³

1977

Pathophysiol Haemos Thromb

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show abstract

“…From each euglobulin fraction, three drops of 30//I each were placed onto the surface of fibrin plates [12]. After incubation of the plates at 37 °C for 18 h on carefully leveled shelves in a Heraeus incubator, type FB 420 (vibratory motions of the incubator being avoided), two perpendicular diameters of each lysed zone were determined with a device especially designed for this purpose [11], The mean of the dia meters was taken as the diameter of the zone. Noncircular zones seldom appeared and were excluded.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Cl Inactivator and Other Proteinase Inhibitors in Euglobulin Fractions and Their Influence on Fibrinolytic Activity

Kluft¹

1976

Pathophysiol Haemos Thromb

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Considerable amounts of Cl inactivator and inter-α-trypsin inhibitor precipitate during euglobulin fractionation of human plasma. The amount precipitated depends on the ionic strength and the pH during the fractionation procedure. In contrast, α₂-anti-trypsin, α₂-macroglobulin and antithrombin III are present in euglobulin fractions in trace amounts only. The fibrinolytic activity of the euglobulin fractions is inhibited by the endogenous Cl inactivator, particularly as shown by comparison of normal and hereditary angioneurotic edema (HANE) plasma.

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“…Fibrin plates were freshly made each day and left for a few hours before application of the euglobulin solutions, from each of which 30 pi were placed in triplicate on the surface of the fibrin by an adjustable pipette with disposable tips (Finnpipette®, Helsinki). After incubation for 17 h at 37 °C on a level glass plate in a moist incubator (Heraeus B 5060), the activity was determined using as a measure the amount of dissolved fibrin expressed arbitrarily by the product (in mm2) of two perpendicular diameters of the lyzed zones conveniently read by the device described elsewhere [ 17] with a precision of ± 0.5 mm and calculating the mean of the triplicates. Duplicate euglobulin precipitations and assays were made from each plasma sample and the mean was recorded as the final value.…”

Section: Methodsmentioning

confidence: 99%

Sequential Study of Plasma Euglobulin Fibrinolytic Activity during the Normal Menstrual Cycle and in Women on Oral Contraceptives Low in Estrogen

Jespersen¹

1983

Gynecol Obstet Invest

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Determinations of plasma euglobulin fibrinolytic activity were made in samples of plasma collected during one normal or hormone-simulated cycle in 15 young, normal women and in 11 women using an oral contraceptive low in estrogen. Distinct, individual levels caused the variations of fibrinolytic activity to be larger within the group than individually, and the activity showed only small changes during the cycle. The hormone group showed a marked increase in fibrinolytic activity with distinct fluctuations related to the phase of the cycle. This increase corresponded to that previously reported to be caused by oral contraceptives containing higher concentrations of estrogen.

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A Simple Device for Measuring Diameters of Fibrinolysis Zones on Fibrin Plates

Cited by 5 publications

References 4 publications

Inhibition of the Fibrinolytic System in Behçet’s Disease?

Inhibition of the Fibrinolytic System in Behçet’s Disease?

Occurrence of Cl Inactivator and Other Proteinase Inhibitors in Euglobulin Fractions and Their Influence on Fibrinolytic Activity

Sequential Study of Plasma Euglobulin Fibrinolytic Activity during the Normal Menstrual Cycle and in Women on Oral Contraceptives Low in Estrogen

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