A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section
            <i>Flavi</i>
            Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger

Chang, Perng‐Kuang

doi:10.1128/spectrum.04648-22

Cited by 8 publications

(3 citation statements)

References 64 publications

(96 reference statements)

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“…Although it requires two vectors rather than one, this development represents a strong foundation for future optimizations of the system. With all of the recent CRISPR advancements accelerating mycology research, we expect that other model fungal pathogens will follow suit, as in this new report by PK Chang on a CRISPR-Cas9 with broad applicability in the Aspergillus genera ( 26 ).…”

Section: Recent Examples Of Revolutionary Fungal Genetics Researchmentioning

confidence: 96%

Advanced genetic techniques in fungal pathogen research

Ross,

Santiago-Tirado

2024

mSphere

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Although fungi have been important model organisms for solving genetic, molecular, and ecological problems, recently, they are also becoming an important source of infectious disease. Despite their high medical burden, fungal pathogens are understudied, and relative to other pathogenic microbes, less is known about how their gene functions contribute to disease. This is due, in part, to a lack of powerful genetic tools to study these organisms. In turn, this has resulted in inappropriate treatments and diagnostics and poor disease management. There are a variety of reasons genetic studies were challenging in pathogenic fungi, but in recent years, most of them have been overcome or advances have been made to circumvent these barriers. In this minireview, we highlight how recent advances in genetic studies in fungal pathogens have resulted in the discovery of important biology and potential new antifungals and have created the tools to comprehensively study these important pathogens.

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Section: Recent Examples Of Revolutionary Fungal Genetics Researchmentioning

confidence: 96%

Advanced genetic techniques in fungal pathogen research

Ross,

Santiago-Tirado

2024

mSphere

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“…Aspergillus terreus and Aspergillus niger have been reported to be associated with the formation of available K (Chang, 2023), and fumigation resulted in a decrease in the relative abundance of Aspergillus. However, with increasing recovery time, the relative abundance of Aspergillus recovered to the pre-fumigation levels.…”

Section: Effect Of Soil Fumigation On Microbial Community Assemblymentioning

confidence: 99%

Long-term effects of chloropicrin fumigation on soil microbe recovery and growth promotion of Panax notoginseng

Wang

et al. 2023

Front. Microbiol.

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IntroductionPanax notoginseng is a precious Chinese medicinal material. Soil fumigation can control soil-borne disease and overcome the continuous cropping obstacles of P. notoginseng. However, chloropicrin (CP) fumigation can kill non-target soil microorganisms and reduce microbial diversity, but the long-time impacts of CP fumigation on soil microbial are less reported.MethodsWe studied the long-term effects of CP fumigation on soil microbes with high-throughput gene sequencing, and correlated the changes in the composition of microbial communities with environmental factors like soil physicochemical properties and soil enzyme activities. This study mainly focuses on the recovery characteristics of soil microbe after soil fumigation by evaluating the ecological restoration of P. notoginseng soil, its sustained control effect on plant diseases, and its promotion effect on crop growth by focusing on the CP fumigation treatment.ResultsThe results showed that CP fumigation significantly increased soil available phosphorus (P) to 34.6 ~ 101.6 mg/kg and electrical conductivity (EC) by 18.7% ~ 34.1%, respectively. High-throughput gene sequencing showed that soil fumigation with CP altered the relative abundance of Trichoderma, Chaetomium, Proteobacteria, and Chloroflexi in the soil while inhibiting a lot of Fusarium and Phytophthora. The inhibition rate of Phytophthora spp. was still 75.0% in the third year after fumigation. Fumigation with CP enhanced P. notoginseng’s survival rate and stimulated plant growth, ensuring P. notoginseng’s healthy in the growth period. The impact of fumigation on microbial community assembly and changes in microbial ecological niches were characterized using normalized stochasticity ratio (NST) and Levins’ niche breadth index. Stochasticity dominated bacterial community assembly, while the fungal community was initially dominated by stochasticity and later by determinism. Fumigation with CP reduced the ecological niches of both fungi and bacteria.ConclusionIn summary, the decrease in microbial diversity and niche caused by CP fumigation could be recovered over time, and the control of soil pathogens by CP fumigation remained sustainable. Moreover, CP fumigation could overcome continuous cropping obstacles of P. notoginseng and promote the healthy growth of P. notoginseng.

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“…Findings from these studies would contribute to better understanding of the biochemical evolution and functional strain during gene function elucidation and epigenetic regulation in loss-of-function (LOF) [ 136 ] or gain-of-function (GOF) research [ 137 ]. This knowledge would also advance the carbon chain expansion process for producing advanced biofuels [ 189 ] and allow for the development of new target production from fungi via CRISPR–Cas9, instead of bacteria such as Streptomyces with fine-tuning the regulation [ 138 – 142 ], Trichoderma reesei with improving cellulase production [ 143 ], and Aspergillus species with improving succinic acid production from renewable biomass [ 144 , 145 ] and yeast with genetic recombination for cellodextrin transport [ 190 ].…”

Section: Technical Approaches For Overcoming Low Efficiency In Carbon...mentioning

confidence: 99%

Biosynthesis pathways of expanding carbon chains for producing advanced biofuels

Lin

2023

Biotechnol Biofuels

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Because the thermodynamic property is closer to gasoline, advanced biofuels (C ≥ 6) are appealing for replacing non-renewable fossil fuels using biosynthesis method that has presented a promising approach. Synthesizing advanced biofuels (C ≥ 6), in general, requires the expansion of carbon chains from three carbon atoms to more than six carbon atoms. Despite some specific biosynthesis pathways that have been developed in recent years, adequate summary is still lacking on how to obtain an effective metabolic pathway. Review of biosynthesis pathways for expanding carbon chains will be conducive to selecting, optimizing and discovering novel synthetic route to obtain new advanced biofuels. Herein, we first highlighted challenges on expanding carbon chains, followed by presentation of two biosynthesis strategies and review of three different types of biosynthesis pathways of carbon chain expansion for synthesizing advanced biofuels. Finally, we provided an outlook for the introduction of gene-editing technology in the development of new biosynthesis pathways of carbon chain expansion.

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A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section Flavi Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger

Cited by 8 publications

References 64 publications

Advanced genetic techniques in fungal pathogen research

Advanced genetic techniques in fungal pathogen research

Long-term effects of chloropicrin fumigation on soil microbe recovery and growth promotion of Panax notoginseng

Biosynthesis pathways of expanding carbon chains for producing advanced biofuels

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