A simple cost effective method for mass propagation of Chrysanthemum morifolium and antibacterial activity assessment of in vitro raised plantlets

Cost Effective Culture Medium for Micropropagation of Paulownia (Paulownia tomentosa Steud.) and Catalpa (Catalpa bignonioides Walt.)

“…In term of high micropropagation costs, there is a need to develop a cost effective system for large scale production of these two important forestry trees wherein the local farmer could purchase the transplants at minimal rates hence guarantee monetary gain to the growers (Pant et al, 2015).…”

Chrysanthemum: A Comprehensive Review on Recent Developments on In Vitro Regeneration

“…The capacity of Chrysanthemum’s shoot apex and nodal explants to regenerate in vitro is overall documented. The culture of nodal segments containing axillary buds involves the exploitation of buds already existing on the parent stock plant, hence providing an efficient method of rapid clonal proliferation enabling the creation of genetically stable and true-to-type progeny [ 46 ]. According to [ 47 ], the nodal segments of Chrysanthemum morifulium L. have been used to design an effective plant regeneration method [ 48 ].…”

“…Furthermore, psyllium husk can be used as a gelling agent in a culture medium because it is a sticky and mucilaginous substance [ 57 , 58 ]. In addition, [ 57 , 58 ] concerns the formation, proliferation, and long-term survival of in vitro shoots [ 18 , 46 , 59 ]. Earlier studies have also mentioned that all gelling agents tested had produced fewer shoots and roots than gellan gum and agar (bacto agar, oatmeal agar, Phytagel, potato dextrose agar, corn starch, and barley starch) [ 60 ].…”

“…Occasionally, organic substances such as amino acids and vitamins are added [ 4 ]. Market sugar was used instead of sucrose due to its similarity to sucrose, which contains 99.98% sucrose and 0.01% reducing sugar (compared to 96–97% sucrose and 0.7–1% reducing sugar in sucrose) [ 46 , 61 ]. Pant et al [ 46 ] developed a modified culture media technique consisting of a full-strength MS medium supplemented with 0.5 mg/L BAP, a solidifying agent.…”

“…According to these findings, BAP was superior in propagating axillary shoots, with 5 M BAP yielding the highest average shoot length and 40 M BAP yielding the highest average number of shoots. The superiority of BAP for inducing axillary buds in C. morifolium was demonstrated by Pant et al [ 46 ]. The ability of low BAP levels itself stimulates shoot growth and proliferation, and callus development in the presence of auxin reflects high endogenous hormone levels in the mother plant [ 46 ].…”