A simple cleaning and fluorescent staining protocol for recent and fossil diatom frustules

Friedrichs, Lars

doi:10.1080/0269249x.2013.799525

Cited by 18 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(i.e., major and minor axes lengths and height of the vertical rim) were determined previously [11]. These overall frustule dimensions were fixed for all the calculations in this study, but the frustule thickness was varied (Table 1), as happens in nature [10,31,32]. …”

Section: Frustule Model Creation Via Cad Modellingmentioning

confidence: 99%

Deformation Modes and Structural Response of Diatom Frustules

Gutierrez¹,

Gordon²,

Dávila³

2017

Jour. Mater. Sci. Eng. Adv. Tech.

View full text Add to dashboard Cite

The ubiquitous diatoms, single-celled algae with porous silica frustule (shell) morphology and features spanning multiple length scales, offer a promising foundation to convert knowledge of biological structures into novel design techniques. Diatom frustules are often found in nature with distinct deformation patterns suggesting the involvement of mechanical interactions in their morphogenesis. Understanding this phenomenon can lead to potential manufacturing techniques based on the micromanipulation of bio-inspired structures. In this study, the mechanics of centric diatom frustules was investigated ALEJANDRO GUTIÉRREZ et al. 106 using the finite element method (FEM) in combination with morphology and material properties obtained from scanning electron microscopy (SEM) and mechanical tests respectively. SEM micrographs of a marine diatom, Coscinodiscus sp., have allowed the classification of specific deformation patterns frequently observed on these frustules. To elucidate the nature of these deformations, a computer aided design (CAD) model approximating a centric diatom frustule was created. Using shear-deformable frustule finite elements and diatom-specific Young's modulus, critical buckling load and modal analysis of the diatom model were performed to investigate possible correlations of the deformation modes with observed morphologies. Results reveal that the first ten deformation modes correlate noticeably well with deformation patterns observed through SEM. Additionally, FEM models were used to study the relation between diatom morphology and mechanical behaviour. A quadratic relation was established between frustule pore size and critical buckling load as well as a cubic relation between frustule thickness and critical buckling load, reported for the first time in this study. The findings in this research provide insights into the mechanical response of diatom frustules that can aid the realization of tailored properties in new bio-inspired materials, in particular for nanotechnology applications, but also for advanced metamaterials and optomechanical devices.

show abstract

Section: Frustule Model Creation Via Cad Modellingmentioning

confidence: 99%

Deformation Modes and Structural Response of Diatom Frustules

Gutierrez¹,

Gordon²,

Dávila³

2017

Jour. Mater. Sci. Eng. Adv. Tech.

View full text Add to dashboard Cite

show abstract

“…acids, bleaching, oxidising or denaturation functions from different chemicals, and thermal treatment. There are few systematic studies with comparisons of different cleaning methods [30] , [31] , [32] , [33] , [34] . Here, a comparative study of two frustule cleaning methods is presented, using carefully described cleaning protocols based on established cleaning principles [35] .…”

Section: Introductionmentioning

confidence: 99%

Diatom frustules as a biomaterial: effects of chemical treatment on organic material removal and mechanical properties in cleaned frustules from two Coscinodiscus species

et al. 2016

View full text Add to dashboard Cite

The three-dimensional structure of silica diatom frustules offers a great potential as nanoporous material for several nanotechnological applications, but the starting point for these applications is the ability to obtain clean frustules with sufficient mechanical strength and intact structure. Here, frustules from the diatoms Coscinodiscus centralis Ehrenberg and C. wailesii Gran et Angst are characterized with respect to their structural integrity, content of residual organic biomaterial and their mechanical properties after two cleaning methods using either hydrogen peroxide as oxidizing agent or a combination of a surfactant (sodium dodecyl sulphate) and a complexing agent. Fluorescence microscopy and energy dispersive spectroscopy (SEM/EDS) analysis revealed clear differences regarding the amount of organic residual within the frustules depending on the cleaning process, with little organic material left after the oxidizing method. This method, however, induced a partial cracking of the frustules suggesting an embrittlement due to the cleaning. Nanoindentation confirmed this and showed that the oxidizing method resulted in more brittle frustules compared to the surfactant/complexing method. More efficient cleaning of organic biomaterial may result in more fragile frustules, and the choice of cleaning method must be based on the planned application.

show abstract

“…To test whether strains can be classified in the high versus low rectangularity morphotypes discovered by Kloster et al (2018), morphometric analyses were performed following that reference. Frustules were prepared using household bleach following Friedrichs (2013) and mounted in Norland Optical Adhesive 61 (Norland Products Inc.). Manually selected valves were imaged in 20 focus levels, and the latter combined to extended depth-of-focus images with a Metafer slide scanning system (Metasystems Gmbh).…”

Section: Morphometricsmentioning

confidence: 99%

Adaptive divergence across Southern Ocean gradients in the pelagic diatom Fragilariopsis kerguelensis

et al. 2020

View full text Add to dashboard Cite

The Southern Ocean (SO) surrounds Antarctica in a continuous belt linking the three main ocean basins. With the exception of the Weddell and Ross gyres, circumpolar currents dominate surface water flow: while in the north, the powerful Antarctic Circumpolar Current (ACC) carries water masses eastward, easterlies along the coast drive southern water masses to the west. These mainly circumpolar currents are crossed by strong meridional gradients in several environmental conditions of importance for planktonic life, including temperature, light and macronutrient concentrations (Pollard, Lucas,

show abstract

A simple cleaning and fluorescent staining protocol for recent and fossil diatom frustules

Cited by 18 publications

References 50 publications

Deformation Modes and Structural Response of Diatom Frustules

Deformation Modes and Structural Response of Diatom Frustules

Diatom frustules as a biomaterial: effects of chemical treatment on organic material removal and mechanical properties in cleaned frustules from two Coscinodiscus species

Adaptive divergence across Southern Ocean gradients in the pelagic diatom Fragilariopsis kerguelensis

Contact Info

Product

Resources

About