A simple but yet novel stain in the diagnosis of onychomycosis

Nargis, Thansiha; Shenoy, Manjunath Mala; Bejai, Vishal; Gopal, Vaishnavi

doi:10.4103/ijdpdd.ijdpdd_19_17

Cited by 4 publications

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“…[ 8 ] Chicago sky blue staining was also performed for increasing the sensitivity of the diagnosis. [ 9 ] Patients with other dermatological disorders that are likely to cause similar nail changes such as psoriasis, lichen planus, and erythroderma were excluded. Patients with tinea unguium associated with paronychia were not counted so that the possibility of candida and non-dermatophyte mold (NDM) onychomycosis was eliminated.…”

Section: Methodsmentioning

confidence: 99%

Role of tinea unguium and other factors in chronic and recurrent dermatophytosis: A case control study

Kalekhan

Asfiya

Shenoy

et al. 2020

Indian Dermatol Online J

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Section: Methodsmentioning

confidence: 99%

Role of tinea unguium and other factors in chronic and recurrent dermatophytosis: A case control study

Kalekhan

Asfiya

Shenoy

et al. 2020

Indian Dermatol Online J

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“…[5] The superior highlighting of fungal elements compared to KOH decreases the screening time and gives quick results which can be very useful in busy outpatient departments and diagnostic laboratories with heavy work load. [1314] This simple time saving method is also of great help in cases of inflammatory tinea capitis where prompt and accurate treatment helps in preventing disfiguring cicatricial alopecia. [9] Instantaneous results in less than 5 min can be obtained by a slight modification of the staining technique by flame heating the CSB stained slide.…”

Section: Discussionmentioning

confidence: 99%

A novel contrast stain for the rapid diagnosis of dermatophytoses: A cross-sectional comparative study of chicago sky blue 6b stain, potassium hydroxide mount and culture

Baddireddy

Poojary

2019

Indian J Dermatol

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Background: The current upsurge of complicated dermatophytosis in India and other parts of the world has necessitated the development of rapid and accurate diagnostic techniques. Conventional methods such as potassium hydroxide (KOH) mount lack color contrast and require a trained eye, whereas fungal culture is laborious and costly. Chicago sky blue 6B (CSB) a new contrast stain promises to be a reliable and rapid diagnostic method. It contains 1% CSB stain and is used with 10% KOH as clearing agent. Aims and Objectives: The objective of this study is to compare the efficacy of a novel contrast stain (CSB) and KOH mount in the diagnosis of dermatophytoses with culture as the reference method. Methods: The infected skin scrapings, nail clippings, and hair specimens from 100 patients, with clinically diagnosed dermatophytoses, were subjected to KOH mount, CSB stain, and culture on Sabouraud's dextrose agar. Using fungal culture as the reference standard, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of CSB stain and KOH mount were determined. An inter-rater reliability analysis was performed using the Cohen's κ statistic to determine agreement amongst the different diagnostic modalities. Results: Of the 100 cases, the CSB stain and KOH mount were positive in 85 (85%) and 70 (70%) cases, respectively. Cultures on Sabouraud's agar yielded growth in 59 (59%) cases. The sensitivity, specificity, PPV, NPV, and accuracy of CSB stain were 98%, 34%, 68%, 93%, and 72%, respectively. The same for KOH mount were 71%, 32%, 60%, 43%, and 55%, respectively. Conclusion: CSB stain is a simple, rapid, sensitive, accurate, and inexpensive office-based method with qualitatively superior demonstration of dermatophytes compared to KOH mount.

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Evaluation of fungal elements in dystrophic nails using Chicago sky blue stain

Aithal

Revathi

2021

Indian J Dermatopathol Diagn Dermatol

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A simple but yet novel stain in the diagnosis of onychomycosis

Cited by 4 publications

References 0 publications

Role of tinea unguium and other factors in chronic and recurrent dermatophytosis: A case control study

Role of tinea unguium and other factors in chronic and recurrent dermatophytosis: A case control study

A novel contrast stain for the rapid diagnosis of dermatophytoses: A cross-sectional comparative study of chicago sky blue 6b stain, potassium hydroxide mount and culture

Evaluation of fungal elements in dystrophic nails using Chicago sky blue stain

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