A simple arrangement for the concentration of protein solutions

Hofsten, Bengt von; Falkbring, S.O.

doi:10.1016/0003-2697(60)90042-7

Cited by 49 publications

(6 citation statements)

References 2 publications

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“…After exhaustive dialysis against distilled water, the precipitate which formed (mainly Fc) was removed by centrifugation. The Fab solution was then concentrated by ultrafiltration (Hofsten and Falkbring, 1961). Phage Inactivation Technique.…”

Section: Methodsmentioning

confidence: 99%

Extrinsic Cotton effect and immunological properties of the p-azobenzenearsonate hapten attached to a helical amino acid copolymer

Conway-jacobs¹,

Schechter²,

Sela³

1970

Biochemistry

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The p-azobenzenearsonate (Ars) hapten was coupled to two synthetic carriers. One was an ordered copolymer possessing the repeating sequence L-tyrosyl-L-alanyl-Lglutamic acid [(TAG),,], and an -helical conformation under physiological conditions. The other one was a random copolymer of L-tyrosine, L-alanine, and L-glutamic acid [(TAG),].The p-azobenzenearsonate group was attached, on the average, to one of every 26 tyrosine residues in the ordered copolymer, and to one in every 7 tyrosine residues in the random

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Section: Methodsmentioning

confidence: 99%

Extrinsic Cotton effect and immunological properties of the p-azobenzenearsonate hapten attached to a helical amino acid copolymer

Conway-jacobs¹,

Schechter²,

Sela³

1970

Biochemistry

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“…100 ml. by ultrafiltration; further concentration was effected by pressure dialysis (Hofsten & Falkbring, 1960) against 0-1 M-potassium phosphate buffer, pH 7-0, containing KCI (0-2M).…”

Section: Standard Enzymic Assaysmentioning

confidence: 99%

The enzymic formation of aminoacetone from threonine and its further metabolism

Green¹,

Elliott²

1964

Biochemical Journal

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“…It was then passed through a column of Sephadex G-100 or G-200 equilibrated with 1 n propionic acid at 4°. The fractions obtained from the Sephadex G-100 column were dialyzed against 0.14 M NaCl for 4 hours, concentrated by ultrafiltration (Hofsten and Falkbring, 1960), and tested by agar-gel diffusion with sheep antiserum against rabbit 7-globulin (Burroughs Wellcome, Engl.). Only fraction A gave a line with this antiserum.…”

Section: Fragmentation Of Antibodies Bymentioning

confidence: 99%

Isolation and Fragmentation of Antibodies to Polytyrosyl Gelatin^*

Sela¹

1964

Biochemistry

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Antibodies to polytyrosyl gelatin were isolated immunospecifically by the digestion of the antigen-antibody precipitate with collagenase. Pieces of the antigen which remained bound to the antibodies and blocked their activity were removed by passing the antibody preparation through a Sephadex column at acid pH. The antibodies thus isolated were 6.5 S y-globulin and precipitated with added fresh antigen. By using radioactively labeled antigen it was possible to follow the antibody-combining sites by means of the pieces of the antigen which remained bound to the antibody before the passage through Sephadex. These antigen pieces were associated with fragments I and II of the antibody after fragmentation with papain, while fragment III was devoid of such pieces. After reduction with mercaptoethanol in aqueous solution and the separation of the subunits A and B of the antibody no radioactive pieces of the antigen were found in fraction B. The protein in fraction A, on the other hand, was associated with antigen pieces. Several alternative explanations for the presence of the antigen pieces in this fraction are discussed.

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A simple arrangement for the concentration of protein solutions

Cited by 49 publications

References 2 publications

Extrinsic Cotton effect and immunological properties of the p-azobenzenearsonate hapten attached to a helical amino acid copolymer

Extrinsic Cotton effect and immunological properties of the p-azobenzenearsonate hapten attached to a helical amino acid copolymer

The enzymic formation of aminoacetone from threonine and its further metabolism

Isolation and Fragmentation of Antibodies to Polytyrosyl Gelatin^*

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A simple arrangement for the concentration of protein solutions

Cited by 49 publications

References 2 publications

Extrinsic Cotton effect and immunological properties of the p-azobenzenearsonate hapten attached to a helical amino acid copolymer

Extrinsic Cotton effect and immunological properties of the p-azobenzenearsonate hapten attached to a helical amino acid copolymer

The enzymic formation of aminoacetone from threonine and its further metabolism

Isolation and Fragmentation of Antibodies to Polytyrosyl Gelatin*

Contact Info

Product

Resources

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Isolation and Fragmentation of Antibodies to Polytyrosyl Gelatin^*