A simple apparatus for electrokinetic removal of sodium dodecyl sulfate from protein digests

Pandigamage, Pravinda; Wilson, Richard; Quirino, Joselito P.

doi:10.1016/j.chroma.2020.461443

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…A special technique of voltage polarity switching t‐ITP made possible full capillary sample loading for CZE‐UV separation of five peptide standards (kemptide, bradykinin, angiotensin I, neurotensin, and angiotensin II) dissolved at 2 μM concentration in the leading electrolyte (LE) composed of 25 mM ammonium acetate, pH 4 [72]. A simple device was developed for electrokinetic removal of SDS to allow CE‐MS analysis of complex peptide mixture without MS ion‐source contamination by this detergent [84]. A thiol‐ene microfluidic chip enabled a fast on‐chip sample clean‐up, separation, and ESI‐MS detection of peptides and proteins in complex biomatrices [85].…”

Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.

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Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

View full text Add to dashboard Cite

show abstract

A simple apparatus for electrokinetic removal of sodium dodecyl sulfate from protein digests

Cited by 1 publication

References 26 publications

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

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