A simple and versatile strategy for sensitive SIDA-UHPLC-MS/MS analysis of Alternaria toxins in olive oil

Lin, Hong-Lin; Ni, Lei; Chen, Huafeng; Xu, Weisheng

doi:10.1016/j.aca.2022.340451

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…These can also be transferred to table olives and olive oil. 9,10 Besides the fungi, insect pests are also of great relevance. Some pests as e.g.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21] However, not so many methods were reported for pesticides determination in table olives 15,21,22 and even less were reported for mycotoxins. Since there is evidence that mycotoxins can contaminate olives [23][24][25] and olive oils, [26][27][28][29][30][31][32][33] it can be considered a concerning topic.…”

Section: Introductionmentioning

confidence: 99%

“…Among the mycotoxins found are aatoxin B1 at concentrations up to 2.4 mg kg −1 , 31,32 alternariol (from 3.0 to 28.0 mg kg −1 ), 26,27,30,33 alternariol monomethyl ether (up to 14.0 mg kg −1 ) 26,30 ochratoxin A (up to 17.7 mg kg −1 ), 31,32 zearalenone (up to 7.1 mg kg −1 ), 26,28,29 aatoxins G1 (up to 1.9 mg kg −1 ) and G2 (up to 6.8 mg kg −1 ), 28 besides sterigmatocystin, tentoxin, beauvericin, mycophenolic acid, enniatin A, A 1 , B and B 1 26 and tenuazonic acid. 30 In contrast, the subject of mycotoxins contamination in olives has not yet been explored so deeply, although a few studies are available in the literature. Khalil, Hashem and Abdelaziz (2019) 25 isolated mycotoxigenic Penicillium species from green table olives from Saudi Arabia.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to pesticides, mycotoxins contamination has also been detected in olive oils. Among the mycotoxins found are aflatoxin B1 at concentrations up to 2.4 μg kg −1 , 31,32 alternariol (from 3.0 to 28.0 μg kg −1 ), 26,27,30,33 alternariol monomethyl ether (up to 14.0 μg kg −1 ) 26,30 ochratoxin A (up to 17.7 μg kg −1 ), 31,32 zearalenone (up to 7.1 μg kg −1 ), 26,28,29 aflatoxins G1 (up to 1.9 μg kg −1 ) and G2 (up to 6.8 μg kg −1 ), 28 besides sterigmatocystin, tentoxin, beauvericin, mycophenolic acid, enniatin A, A 1 , B and B 1 26 and tenuazonic acid. 30…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Improving analytical performance for pesticides and mycotoxins determination in Brazilian table olives: one extraction and one analysis

Reichert,

Pizzutti,

Jänisch

et al. 2024

Anal. Methods

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“…These can also be transferred to table olives and olive oil. 9,10 Besides the fungi, insect pests are also of great relevance. Some pests as e.g.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improving analytical performance for pesticides and mycotoxins determination in Brazilian table olives: one extraction and one analysis

Reichert,

Pizzutti,

Jänisch

et al. 2024

Anal. Methods

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show abstract

“…Using the HPLC under rather adverse storage conditions (rain, humidity and high temperatures) and the simultaneous presence of the toxins, which tends to increase the risks of toxicity, underlines the importance of having a good control over the storage of olives after harvest [10][11][12]. Other recent studies have led to the use of innovative techniques that allow simultaneous purification and enrichment in a single sample extraction step [13].…”

Section: Introductionmentioning

confidence: 99%

Ochratoxin A and Aflatoxins: Fine-Tuning to the ELISA Test on Table Olives

Cammerata,

Del Frate,

Monforte

et al. 2023

Preprint

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This study is aimed to point out the possible application of the Enzyme-Linked ImmunoSorbent Assay (ELISA) test on table olives. Because olives, like many other foods, are not free from mycotoxin contamination, the main focus of this work was assessing the presence or absence of aflatoxins and ochratoxin A. The work was carried out in two phases. In the first phase, tests were carried out on commercial olives to assess the efficacy of the method and the kit (Veratox® 'Neogen' and AgraQuant® 'Romer Labs') by comparing them with the Liquid Chromatography coupled to a mass-mass spectrometer (LC-MS/MS); in order to understand if there were any interferences in antigen-antibody recognition; subsequently, mycotoxins were inoculated on the same samples with a known quantity, in order to assess the concentrations obtained and compare them to the expected ones. The second step involved modifying the method, so to improve the recovery of inoculated mycotoxins in the samples under study. Afterwards we evaluated the presence of mycotoxins in table olives supplied in the project. The results showed the effectiveness of the ELISA test, especially in the mycotoxin recovery phase. Indeed, after the modification of the mycotoxin extraction method, recoveries increased from 55% to over 70% for aflatoxins, and from 40% to over 55% for ochratoxin A. When analysing the samples provided by the above-mentioned company, the results showed some false positives, in particular in relation to ochratoxin A ’s analysis, mainly related to the first sample submission. Taking this into account, the ELISA test can be considered as an initial screening method applicable as a control programme for the evaluation of mycotoxins in olives which helps by saving time and costs. It should always be kept in mind that, if positive values should emerge, the LC-MS/MS analysis should be carried out.

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The characteristics, occurrence, and toxicological effects of alternariol: a mycotoxin

Saleh,

Zeidan,

Abu-Dieyeh

2024

Arch Toxicol

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Alternaria species are mycotoxin-producing fungi known to infect fresh produce and to cause their spoilage. Humans get exposed to fungal secondary metabolites known as mycotoxin via the ingestion of contaminated food. Alternariol (AOH) (C14H10O5) is an isocoumarins produced by different species of Alternaria including Alternaria alternata. AOH is often found in grain, fruits and fruits-based food products with high levels in legumes, nuts, and tomatoes. AOH was first discovered in 1953, and it is nowadays linked to esophagus cancer and endocrine disruption due to its similarity to estrogen. Although considered as an emerging mycotoxin with no regulated levels in food, AOH occurs in highly consumed dietary products and has been detected in various masked forms, which adds to its occurrence. Therefore, this comprehensive review was developed to give an overview on recent literature in the field of AOH. The current study summarizes published data on occurrence levels of AOH in different food products in the last ten years and evaluates those levels in comparison to recommended levels by the regulating entities. Such surveillance facilitates the work of health risk assessors and highlights commodities that are most in need of AOH levels regulation. In addition, the effects of AOH on cells and animal models were summarized in two tables; data include the last two-year literature studies. The review addresses also the main characteristics of AOH and the possible human exposure routes, the populations at risk, and the effect of anthropogenic activities on the widespread of the mycotoxin. The commonly used detection and control methods described in the latest literature are also discussed to guide future researchers to focus on mitigating mycotoxins contamination in the food industry. This review aims mainly to serve as a guideline on AOH for mycotoxin regulation developers and health risk assessors.

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A simple and versatile strategy for sensitive SIDA-UHPLC-MS/MS analysis of Alternaria toxins in olive oil

Cited by 5 publications

References 30 publications

Improving analytical performance for pesticides and mycotoxins determination in Brazilian table olives: one extraction and one analysis

Improving analytical performance for pesticides and mycotoxins determination in Brazilian table olives: one extraction and one analysis

Ochratoxin A and Aflatoxins: Fine-Tuning to the ELISA Test on Table Olives

The characteristics, occurrence, and toxicological effects of alternariol: a mycotoxin

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