A simple and sensitive UFLC-fluorescence method for endocrine disrupters determination in marine waters

Lisboa, Normando S.; Fahning, Cristiane S.; Cotrim, Gabriel; Anjos, Jeancarlo Pereira dos; Andrade, Jailson B. de; Hatje, Vanessa; Rocha, Gisele O. da

doi:10.1016/j.talanta.2013.08.006

Cited by 41 publications

(20 citation statements)

References 60 publications

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“…Nevertheless, it is important to point out that most of the works have employed 230 nm as excitation wavelength 29,33,37 which has a suitable response to river and drinking water samples. Nonetheless, wastewater samples showed high interference on fluorescence signal at 230 nm (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

“…Despite that, these instruments are expensive and require high investment costs for their maintenance. 29 High-performance liquid chromatography coupled with UV (HPLC-UV) [30][31][32] and fluorescence detection (HPLC-FLD) 26,29,[33][34][35] have been reported as low-cost alternatives to HPLC-MS analysis, used to determine estrogens with quantification limits compatible with the concentration of estrogens in wastewater and natural waters. Lopes et al 36 showed the determination of E1 and E2 by SPE-HPLC-FLD in natural (600 ng L -1 of E1 in surface water, 6 ng L -1 of E2 in river spring) and treated waters (6.8 ng L -1 of E2) in São Paulo (Brazil).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection

Liz¹,

Amaral²,

Stets³

et al. 2016

J. Braz. Chem. Soc.

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A sensitive high-performance liquid chromatography-fluorescence detection method (HPLC-FLD) was developed and validated for 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) determination in wastewater samples. Sample preparation was performed by solid-phase extraction (SPE) on Hypersep C18 cartridges. Conditioning and elution solvents and sample volume were investigated by a 2 3 factorial design for both analytes. The pre-concentration factor was 2500 times, allowing to quantify 5.0 ng L -1 for both hormones. The SPE-HPLC-FLD method was successfully applied to detect and quantify trace amount of target compounds in raw and treated wastewater samples. E2 was found below limit of quantification in influent and effluent samples. Nevertheless, the recoveries of E2 in standard addition experiments were between 41 and 72%, with relative standard deviations between 4 and 16%. An important matrix effect was observed in the determination of EE2, with fluorescence signal suppression. Keywords: estrogens, SPE, HPLC, fluorescence, wastewater IntroductionContamination of water resources represents a worldwide concern. Excessive population growth with the inefficient wastewater collection, particularly in developing countries, low treatment efficiency and inadequate sewage disposal, are the common causes of water contamination.1 Endocrine disrupting compounds (EDCs), especially estrogens, are chemical species that have negative activity on the endocrine system in humans 2,3 and animals. 4,5 Estrone (E1), 17β-estradiol (E2) and estriol (E3) are estrogens naturally produced by mammals and excreted mainly by pregnant and menstrual women via urine and feces.1 Otherwise, 17α-ethinylestradiol (EE2) is a synthetic estrogen widely used as contraceptive and for therapeutic purposes such menopausal syndrome and prostate cancer control. 6 Despite of being unregulated contaminants in environmental waters, they have already been included in the New Contaminant Candidate List-3 of the U.S.EPA and in the list of priority substances of the EU Water Framework Directive. 7,8 These compounds have been detected in wastewater treatment plants (WWTP), [9][10][11][12] in natural waters 9,13 and, even in water supplies.14 Since 1990, estrogenic activity has been detected in many countries. For instance, E1, E2, and EE2 were found in concentrations about ng L -1 in the effluent from a sewage treatment plant in the UK. 15 In Brazil, monitoring studies of natural estrogens in domestic sewage also were initiated in the 90s. 16 This study revealed estrogen concentrations between 20 and 40 ng L -1 and only partial removal by conventional treatments based on biological filter and activated sludge systems. Since then, other monitoring works reported the presence of endocrine disruptors in surface waters in Brazil. Sodré et al. 17 The estrogens monitoring in sewage and natural waters is a challenge due to the low concentration of the analytes and the complexity of the environmental matrices. Most of the reported methods to estrogens determination in aque...

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection

Liz¹,

Amaral²,

Stets³

et al. 2016

J. Braz. Chem. Soc.

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“…R 2 was 0.9980, it is assumed that an ideal data fit was achieved whenever R 2 was equal to or greater than 0.99 (Lisboa et al 2013). The limit of detection (LOD), defined as LOD ¼ 3S B =m, where S B and m are the standard deviation of the blank and the slope of the calibration graph, respectively, was 0.072 mg Á mL À1 .…”

Section: Gold(iii) By Ionic Liquid Extraction and Faas 1057mentioning

confidence: 99%

Determination of Gold(III) by Simplified Room-Temperature Ionic Liquid Extraction with Flame Atomic Absorption Spectrometry

Sha

Zhu

2014

Analytical Letters

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A new simplified extraction of gold(III) using a room-temperature ionic liquid prior to determination by flame atomic absorption spectrometry has been developed. The extraction method uses 1-butyl-3-methylimidazolium hexafluorophosphate without a chelating agent. The parameters of the extraction system were investigated in detail. Under the optimized conditions, a linear range of 0.19 to 38.20 lg Á mL À1 , a limit of detection of 0.072 lg Á mL À1 , an enrichment factor of 10.0, and an extraction capacity of 6.6 mg Á g À1 were obtained. The extraction mechanism and the selectivity of 1-butyl-3-methylimidazolium hexafluorophosphate for gold(III) were also investigated. The method was applied for the determination of gold(III) in water samples with satisfactory results.

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“…Among the emerging contaminants, a number of compounds have been receiving special attention from the scientific community, due to their potential capacity of interfering with the functioning of the endocrine system of organisms. The literature uses several names for these compounds: xenobiotics, pseudo-estrogens, pseudo-androgens, endocrine disruptors (ED), and interfering endocrines (Lisboa et al, 2013;Menzies et al, 2013;Grassi et al, 2013;Ghiselli & Jardim, 2007;Lathers, 2002;Kardinaal et al, 1997). Many of them are not degraded or broken down by any biochemical and/or natural photochemical pathways and may also undergo bioaccumulation and/or biomagnification (Colin et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Endocrine disruptors: strategies for determination and occurrence in marine environments

Cotrim¹,

Fahning²,

Rocha³

et al. 2016

RGCI

Self Cite

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Research examining the occurrence of endocrine disruptors (ED) in the marine environment has substantially increased. These contaminants have been observed in several environmental compartments and matrices, and they may cause severe adverse effects in humans and ecosystems. In this study more than 240 papers investigating the analytical developments regarding the analysis on ED in environmental matrices and the occurrence of these compounds were critically evaluated. Modern sample preparation procedures aiming the use of minimal sample manipulation, minimal amount of solvents and energy according to the Green Chemistry principles are widely used. The ED in marine environments occurs in trace concentrations and their quantification still represents a challenge. The effects of these contaminants in marine ecosystems are poorly understood. However, due to their large use, it is predicted that new analytical developments to deal with ED contamination will promote a large increase in the number of scientific publications in the near future. Regulations and mitigation measures for the presence of these contaminants in the environment are still scarce and need to be quickly implemented to reduce potential future adverse effects on ecosystem services of coastal environments. Cotrim et al. (2016) 300 RESUMO Pesquisas que examinam a ocorrência de interferentes endócrinos (IE) no ambiente marinho têm aumentado substancialmente. Estes contaminantes podem causar efeitos adversos em seres humanos e nos ecossistemas e têm sido observados em vários compartimentos e matrizes ambientais. Neste estudo, mais de 240 trabalhos que relataram o desenvolvimento analítico de IE em diversas matrizes e a ocorrência destes compostos no ambiente marinho foram criticamente avaliados. Procedimentos de preparo de amostra, visando a mínima manipulação, a mínima quantidade de solventes e energia de acordo com os princípios de Química Verde estão sendo amplamente utilizados. Os IE em ambientes marinhos ocorrem em concentrações traço e sua quantificação ainda representa um grande desafio. Como resultado, os efeitos desses contaminantes em ecossistemas marinhos ainda são mal compreendidos. No entanto, devido ao amplo uso destes compostos é previsto que novos desenvolvimentos analíticos para a determinação de IE irão promover um grande aumento no número de publicações científicas no futuro. Regulações e medidas mitigadoras para a ocorrência destes contaminantes ainda são bastante reduzida e precisam ser rapidamente implementadas para reduzir os futuros potenciais efeitos adversos nos serviços ecossistêmicos dos ecossistemas costeiros. Journal of Integrated Coastal Zone Management / Revista de Gestão Costeira Integrada, 16(3):299-326 (2016)

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A simple and sensitive UFLC-fluorescence method for endocrine disrupters determination in marine waters

Cited by 41 publications

References 60 publications

Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection

Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection

Determination of Gold(III) by Simplified Room-Temperature Ionic Liquid Extraction with Flame Atomic Absorption Spectrometry

Endocrine disruptors: strategies for determination and occurrence in marine environments

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