A simple and sensitive saturation assay method for the measurement of adenosine 3′:5′-cyclic monophosphate

Hepatic action of vasopressin: Lack of a role for adenosine‐3′,5′‐cyclic monophosphate

“…Cyclic AMP was measured by a radioisotope saturation assay [6]. Samples of liver were quick-frozen in liquid Nz, and extracted in 10 vols (w/v) of 6% (w/v) TCA (trichloroacetic acid); 200-400 1.11 of extract was washed 4 times with water-saturated diethyl ether to remove the TCA, dried and taken up in buffer containing theophylline and mercapto-ethanol for assay [6].…”

“…Samples of liver were quick-frozen in liquid Nz, and extracted in 10 vols (w/v) of 6% (w/v) TCA (trichloroacetic acid); 200-400 1.11 of extract was washed 4 times with water-saturated diethyl ether to remove the TCA, dried and taken up in buffer containing theophylline and mercapto-ethanol for assay [6]. Perfusion medium was spun to remove erthrocytes, and mixed with an equal volume of buffer, heated at 100°C for 3 min and centrifuged to remove protein.…”

Catecholamine receptor sensitivity and the regulation of lipolysis in adult diabetes

“…At the end of 5 minutes incubation the accumulation of cyclic AMP was terminated by the addition of the sample to ice-cold 0.7 M HCI. Samples were homogenised in triethanolamine buffer and petroleum ether, and after neutralisation with 2.8 M KOH the extract was stored at -20 ~ C until assayed by a competitive proteinbinding assay [12].…”

Inhibition of thyrotropin‐stimulated cyclic AMP accumulation by human thyroglobulin in human cultured thyroid cells

“…Protein concentration was measured by Azso (el cm -'% -10.0). Intracellular accumulation of CAMP was measured in triplicate in the freezed-dried 2000 X g supernatant of the ethanoltreated homogenate, by saturation analysis using a cytosol protein purified from bovine adrenal cortices [7]. The DNA content was measured in the pellet by a fluorimetric method [8] after complete extraction of lipids.…”